Genetic Analysis of Photoperiod/Thermosensitive Male Sterility in Rice under US Environments

Abstract

Two‐line hybrid rice (Oryza sativa L.) breeding in the United States and China uses photoperiod/thermosensitive genetic male sterility (PTGMS) to produce lines with fertile or sterile pollen, depending on temperature and/or daylength. Although studied in China for >30 yr, genetic analysis of PTGMS in US environments is lacking. We therefore conducted genetic studies of male sterility over 3 yr in five F2 and BC1F2 populations derived from PTGMS line 2008S in Louisiana and Arkansas. Chi‐squared analyses in all populations indicated that sterility was controlled at both locations by two or three recessive genes. Inheritance and expression of PTGMS was found to be influenced by the particular combination of population and year (environment). Sequence comparisons of two regions in PTGMS loci LOC_Os07g12130 and LOC_Os12g36030 from six fertile and sterile lines revealed a diagnostic single‐nucleotide polymorphism (SNP) at each locus. Two‐way ANOVA in two F2 populations inferred that interaction of the two SNP‐containing regions contributed up to 65% variation for sterility in one Louisiana environment. Selective genotyping of the 2008S/CL131 F2 population detected new quantitative trait loci with R2 values ranging from 5 to 27% using single‐marker and composite‐interval mapping. Results from this study indicate that 2008S can be used as parental material for development of two‐line hybrids in Louisiana and Arkansas, but extensive testing of populations across years and locations will be required.

Genetics and Breeding System for Cytoplasmic and Genetic Male Sterility in Rice

“…This greatly reduce the time and resources in making hybrid combination and parental seed production. Moreover, it broadens the available choices of restorers that can generate more combinations which in turn increases the probability of finding the best hybrids [4].…”

“…Studies conducted under US conditions on indica male sterile line 2008S originated from China showed that male sterility is controlled by two or three recessive genes depending on the location and year of planting (Figure 4). When planted in [4].…”

“…However, the gene is yet to be cloned and further study needs to be conducted. De Guzman [4] sequenced both locus in male sterile line 2008S and found both SNPs present. When QTLs were analyzed in two segregating populations using both single marker analysis and interval mapping, each locus and their interaction gives significant effects [25].…”

“…The use of this system is increasing due to the ease in breeding, finding more heterotic combinations, and in seed multiplication of parental male sterile lines. However, hybrid seed production may be catastrophic if there are severe changes in environmental conditions [4]. Both systems proved effective in hybrid rice production which increased yields by up to 20% therefore, increased farm profitability and has contributed significantly in addressing global food security.…”