Molecular mapping of black rot resistance locusXca1boon chromosome 3 inIndian cauliflower (Brassica oleraceavar.botrytisL.)

Abstract

Black rot is the most devastating disease of cauliflower worldwide causing severe damage to crop. The identification of markers linked to loci that control resistance can facilitate selection of plants for breeding programmes. In the present investigation, F 2 population derived from a cross between 'Pusa Himjyoti', a susceptible genotype, and 'BR-161', a resistant genotype, was phenotyped by artificial inoculation using Xcc race 1. Segregation analysis of F 2 progeny indicated that a single dominant locus governed resistance to Xcc race 1 in 'BR-161'. Bulk segregant analysis in resistant and susceptible bulks of F 2 progeny revealed seven differentiating polymorphic markers (three RAPD, two ISSR and two SSR) of 102 markers screened. Subsequently, these markers were used to genotype the entire F 2 population, and a genetic linkage map covering 74.7 cM distance was developed. The major locus Xca1bo was mapped in 1.6-cM interval flanked by the markers RAPD 04 833 and ISSR 11 635 . The Xca1bo locus was located on chromosome 3. The linked markers will be useful for marker-assisted resistance breeding in cauliflower.

Screening of Cabbage (Brassica oleracea L.) Germplasm for Resistance to Black Rot

“…However, the strong resistance source for Xcc race 4 was reported to be originated from A genome while resistance to both races 1 and 4 from B genome, and B. juncea (AB genome) was the most resistant species to races 1 and 4 . To date, several QTLs for black rot resistance have been described in B. oleracea and other B. species (Camargo et al 1995;Cheng et al 2009;Kifuji et al 2013;Izzah et al 2014;Saha et al 2014;Lee et al 2015;Sharma et al 2016). In this study, five reported SSR markers showed polymorphism in banding pattern for R and S lines of the tested cabbage lines.…”

“…Kifuji et al (2013) reported a QTL for black rot resistance using 161 EST-SNP markers and found that QTL-1 located on LG C02 is the major QTL in cabbage. A major black rot resistance locus called Xca1bo has been mapped on Chromosome 03 in Indian cauliflower (Saha et al 2014). Lee et al (2015) reported a genetic linkage map where they identified four QTLs such as BRQTL-C1_1 and BRQTL-C1_2 on LG C01, BRQTL-C3 on LG C03 and BRQTL-C6 on LG 06.…”

Computational Prediction of Effector Proteins in Fungi: Opportunities and Challenges

“…In the latter case, fungal pathogens are known to quickly develop resistance to most chemicals and the use of fungicides is generally perceived as negative for human health and the environment ( Van de Wouw et al, 2014 ; Balint-Kurti and Holland, 2015 ). For this reason, genetic approaches are considered safer and more durable, and considerable efforts are deployed toward the identification and introgression of resistance genes into plant material ( Channamallikarjuna et al, 2010 ; Raman et al, 2012 ; Singh et al, 2012 ; Saha et al, 2014 ). However, the use of a single source of resistance also brings tremendous selection pressure on the pathogen, and the resistance often breaks down quite rapidly ( Kutcher et al, 2013 ; Van de Wouw et al, 2014 ).…”

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

“…Tuno et al 42 analyzed BR resistance QTLs and the major QTL XccBo(Reiho)2 was detected on C8. Saha et al 43 mapped the Xcc race 1 resistance gene Xca1bo in the cauliflower line BR-161 within a 1.6 cM interval. Sharma et al 44 first developed a B. carinata F 2 mapping population and mapped the BR race 1 resistance locus Xca1bc to a 6.6 cM interval.…”