Molecular mapping of a novel male-sterile gene msNJ in soybean [Glycine max (L.) Merr.]

Nie, Zhixing; Zhao, Tuanjie; Liu, Meifeng; Dai, Junjie; He, Tingting; Lyu, Duo; Zhao, Jinming; Yang, Shouping; Gai, Junyi

doi:10.1007/s00497-019-00377-6

Cited by 17 publications

(18 citation statements)

References 24 publications

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“…Plant male sterile mutants are important materials for studying the anther development mechanisms and crucial tools for crop hybrid breeding. So far, 13 genetic loci in soybean have been reported to condition male sterile phenotype independently when they are mutated, including ms1-ms9, msp, msMOS, mst-M, and ms NJ (Yang et al 2014;Zhao et al 2019;Nie et al 2019;Thu et al 2019), but only ms4 has been molecularly identi ed, which encodes a PHD-nger protein and is involved in the meiosis process of microsporocyte (Thu et al, 2019). Amongst these mutants, two ms6 mutants identi ed decades ago exhibit no-pollen phenotypes (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, it has not been applied in soybean as lack of cloned GMS gene. So far, 13 non-allelic genic loci distributed on 7 different chromosomes have been reported to condition anther development in soybean, including ms1-ms9, msp, msMOS, mst-M, and ms NJ (Yang et al 2014;Zhao et al 2019;Nie et al 2019;Thu et al 2019). Mutations at these loci all confer recessive sporophytic male-sterile phenotype.…”

Section: Introductionmentioning

confidence: 99%

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A Single Nucleotide Polymorphism in an R2R3 MYB Transcription Factor Triggers ms6 (Ames1) Male Sterility in Soybean

Yu¹,

Zhao

Li³

et al. 2021

Preprint

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Soybean [Glycine max (L.) Merr.] is an important crop providing vegetable oils and proteins. Increasing demand on soy products heightens the urgency of soybean yield improvement. Hybrid breeding with male sterility system is an effective method to improve crop production. Cloning of genic male sterile (GMS) gene combined with biotechnology method can contribute to constructing GMS-based hybrid Seed Production Technology (SPT) to promote soybean performance and yield. In this research, we identified a soybean GMS locus, GmMS6, by combining bulked segregant analysis (BSA)-sequencing and map-based cloning technology. GmMS6 encodes an R2R3 MYB transcription factor, whose mutant allele in ms6 (Ames1) harbors a single nucleotide polymorphism (SNP) substitution, leading to the 76th Leucine to Histidine change in the DNA binding domain. Phylogenetic analysis demonstrates GmMS6 is a homolog of Tapetal Development and Function 1 (TDF1)/MYB35 that is an anther development key factor co-evolved with angiosperm. It has a recently duplicated homolog GmMS6LIKE (GmMS6L), both of which can rescue the male fertility of Arabidopsis homologous mutant attdf1 while GmMS6L76H cannot, denoting that both proteins are functional and L76 is a critical residue for TDF1’s function. However, compared to anther specific expressed GmMS6, GmMS6L is constitutively expressed at a very low level, explaining deficiency of GmMS6 alone causes pollen abortion. Moreover, the expression levels of major regulatory and structural genes for anther development are significantly decreased in ms6, unveiling that GmMS6 is a core transcription factor regulating soybean anther development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Single Nucleotide Polymorphism in an R2R3 MYB Transcription Factor Triggers ms6 (Ames1) Male Sterility in Soybean

Yu¹,

Zhao

Li³

et al. 2021

Preprint

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“…It is a tool initially developed for allogamous crops; however, application in autogamous plants, such as wheat, oats, rice, and common bean have increased in recent years. In the case of soybean, there are reports of the use of recurrent selection outside of Brazil for grain protein content (Patil et al 2017, Rialch andSharma 2019) and grain yield (Sumarno and Fehr 1982, Posadas et al 2014, Vello and Nazato 2017, Nie et al 2019, Hegstad et al 2019. However, it should be highlighted that for the purpose of expanding the genetic variability of the base population of the recurrent selection program, the authors used exotic germplasm, i.e., germplasm with low adaptability and, likewise, lower frequency of favorable alleles.…”

Section: Discussionmentioning

confidence: 99%

“…With the aim of maximizing resources, as well as efficient use of the time necessary for identification of superior genotypes for recombination, recurrent selection is a useful strategy. Although this strategy has been promoted for breeding of allogamous plants, its use is reported in the literature in autogamous IO Soares et al plants like common bean (Pereira et al 2017a, Anjos et al 2018, Melo et al 2019) and soybean (Posadas et al 2014, Vello and Nazato 2017, Nie et al 2019. The use of this technique for the purpose of increasing grain yield in Brazil has not been reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Genetic and phenotypic parameters associated with soybean progenies in a recurrent selection program

Soares

Bianchi

Bruzi

et al. 2020

Crop Breed. Appl. Biotechnol.

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“…The morphology of anthers from opened flowers of soybean and Arabidopsis was observed under an Olympus CX31 microscope (Japan). Pollen viability of soybean and Arabidopsis was analyzed by I 2 -KI staining (Nie et al, 2019) and Alexander's staining (Ding et al, 2020), respectively. The stamen and pistil length of Arabidopsis was measured with the cellSens software (Olympus, Japan).…”

Section: Gus Staining and Plant Trait Investigationmentioning

confidence: 99%

Comparative Transcriptomics Analysis and Functional Study Reveal Important Role of High-Temperature Stress Response Gene GmHSFA2 During Flower Bud Development of CMS-Based F1 in Soybean

Ding

Guo

et al. 2020

Front. Plant Sci.

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High-temperature (HT) is one of the most important environmental factors that negatively impact the yield of some soybean cytoplasmic male sterility (CMS)-based hybrid (F1) combinations. The response of soybean to HT, especially at the male organ development stage, is poorly understood. To investigate the molecular mechanisms of the response from soybean CMS-based F1 male organ to HT, a detailed transcriptomics analysis was performed during flower bud development of soybean HT-tolerant and HT-sensitive CMS-based F1 combinations (NF1 and YF1) under normal-temperature and HT conditions. Obvious HT damage was observed by subjecting YF1 with HT, such as indehiscent anthers and decreased pollen fertility, whereas the male fertility of NF1 was normal. In total, 8,784 differentially expressed genes (DEGs) were found to respond to HT stress, which were mainly associated with anther/pollen wall development, carbohydrate metabolism and sugar transport, and auxin signaling. The quantitative real-time PCR (qRT-PCR) analysis and substance content detection also revealed that HT caused male fertility defects in YF1 by altering pectin metabolism, auxin, and sugar signaling pathways. Most importantly, the sugar signaling-PIF-auxin signaling pathway may underlie the instability of male fertility in YF1 under HT. Furthermore, HT induced the expression of heat shock factor (HSF) and heat shock protein (HSP) gene families. Overexpression of GmHSFA2 in Arabidopsis can promote the expression of HT protective genes (such as HSP20) by binding to the HSE motifs in their promoters, so as to improve the HT tolerance during flowering. Our results indicated that GmHSFA2 acted as a positive regulator, conferring HT tolerance improvement in soybean CMS-based F1. GmHSFA2 may be directly involved in the activation of male fertility protection mechanism in the soybean CMS-based F1 under HT stress.

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Molecular mapping of a novel male-sterile gene msNJ in soybean [Glycine max (L.) Merr.]

Cited by 17 publications

References 24 publications

A Single Nucleotide Polymorphism in an R2R3 MYB Transcription Factor Triggers ms6 (Ames1) Male Sterility in Soybean

A Single Nucleotide Polymorphism in an R2R3 MYB Transcription Factor Triggers ms6 (Ames1) Male Sterility in Soybean

Genetic and phenotypic parameters associated with soybean progenies in a recurrent selection program

Comparative Transcriptomics Analysis and Functional Study Reveal Important Role of High-Temperature Stress Response Gene GmHSFA2 During Flower Bud Development of CMS-Based F1 in Soybean

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