Molecular mapping and identification of QTL's associated to oat crown rust partial resistance

Barbosa, Marta Martins; Federizzi, Luiz Carlos; Milach, Sandra Cristina Kothe; Martinelli, José Antônio; Thomé, Gladis Cleci Hermes

doi:10.1007/s10681-006-9117-4

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…Rosewarne et al (2006) identified and mapped new AFLP markers associated with the Lr46/Yr29 complex, an important gene to APR in wheat. In oat, which has the largest cereal genome, Barbosa et al (2006) tagged partial resistance to oat crown rust by using the AFLP technique. In our present study, we have tagged 2 new APR genes to leaf rust in wheat by using AFLP.…”

Section: Discussionmentioning

confidence: 99%

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Silva¹,

Brammer²,

Guerra³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian wheat cultivar Toropi. The Toropi/IAC 13 F 2 and F 7 recombinant inbred lines (RILs) were developed in previous studies. Phenotypic analysis of the F 2 and F 7 RILs showed that 2 recessive genes that were temporarily named trp-1 and trp-2 conferred APR in Toropi. In the present study, we used monosomic families and amplified fragment length polymorphism (AFLP), sequence-tagged site, and simple sequence repeat (SSR) markers to map trp-1 and trp-2 on wheat chromosomes. Analysis of the F 2 monosomic RIL showed that trp-1 and trp-2 were located on chromosomes 1A and 4D, respectively. AFLP analysis of the F 7 RIL identified 2 independent AFLP markers, XPacgMcac3 and XPacgMcac6, which were associated with Toropi APR. These markers explained 71.5% of the variation in the phenotypic data in a multiple linear regression model. The AFLP markers XPacg/ Mcac3 and XPacg/Mcac6 were anchored by SSR markers previously mapped on the short arms of chromosomes 1A (1AS) and 4D (4DS), respectively. The trp-2 gene is the first leaf rust resistance gene mapped on wheat chromosome 4DS. The mapping of trp-1 and trp-2 provides novel and valuable information that could be used in future studies involving the fine mapping of these genes, as well as in the identification of molecular markers that are closely related to these genes for markerassisted selection of this important trait in wheat.

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Section: Discussionmentioning

confidence: 99%

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Silva¹,

Brammer²,

Guerra³

et al. 2012

Genet. Mol. Res.

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“…The Canadian A. sterilis accession 1387 is a case in which a single partially dominant gene, Pc69, controls APR (Harder et al, 1984). Currently, six genes have been designated to be APR-conditioning genes in oat, namely Pc27, Pc28, Pc69, Pc72, Pc73 and Pc74 (Table S1) (USDA-ARS CDL, 2016), and more than 25 quantitative trait loci (QTLs) associated with APR have been mapped in oat (Acevedo et al, 2010;Babiker et al, 2015;Barbosa et al, 2006;Jackson et al, 2007;Lin et al, 2014;Portyanko et al, 2005;Zhu and Kaeppler, 2003).…”

Section: Genetic Resistance To Oat Crown Rustmentioning

confidence: 99%

“…As exemplified by QTL identification in the oat lines MN841801 and TAM O-301, the detection of genes for quantitative resistance is expedited by new marker technologies that are proving advantageous in the alleviation of mapping complexities. Newly developed high-throughput genetic marker assay systems, such as SNP, KASP and diversity arrays technology (DArT), are of benefit in oat breeding, with the construction of dense genetic maps enabling the discovery of additional resistance QTLs against crown rust in different oat crosses and RILs (Babiker et al, 2015;Barbosa et al, 2006;Chaffin et al, 2016;Jackson et al, 2007;Zhu and Kaeppler, 2003). In parallel, such systems are also used for genome-wide association study (GWAS) as an alternative route to locate crown rust resistance QTLs (Klos et al, 2017;Montilla-Basc on et al, 2015).…”

Section: Genetic Resistance To Oat Crown Rustmentioning

confidence: 99%

Puccinia coronata f. sp. avenae: a threat to global oat production

Nazareno

Smith

et al. 2017

Molecular Plant Pathology

101

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Most crown rust management strategies involve the use of rust-resistant crop varieties and the application of fungicides. The attainment of the durability of resistance against Pca is difficult as it is a highly variable pathogen with a great propensity to overcome the genetic resistance of varieties. Thus, adult plant resistance is often exploited in oat breeding programmes to develop new crown rust-resistant varieties. Useful website: https://www.ars.usda.gov/midwest-area/st-paul-mn/cereal-disease-lab/docs/cereal-rusts/race-surveys/.

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“…Therefore, it has the potential for efficient gene pyramiding, i.e., combining several important genes in one cultivar. A wide group of molecular markers, including RFLP (Pal et al 2002), RAPD (Penner et al 1993), AFLP (Barbosa et al 2006), SSR (Li et al 2000;Becher 2007), SNP (Chen et al 2006) and silicoDArT (Okoń et al 2018a) have been successfully applied in the selection of valuable oat individuals.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and chromosomal localization of new powdery mildew resistance gene Pm11 in oat

et al. 2019

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The appropriate selection of various traits in valuable plants is very important for modern plant breeding. Effective resistance to fungal diseases, such as powdery mildew, is an example of such a trait in oats. Marker-assisted selection is an important tool that reduces the time and cost of selection. The aims of the present study were the identification of dominant DArTseq markers associated with a new resistance gene, annotated as Pm11 and derived from Avena sterilis genotype CN113536, and the subsequent conversion of these markers into a PCR-based assay. Among the obtained 30,620 silicoDArT markers, 202 markers were highly associated with resistance in the analysed population. Of these, 71 were selected for potential conversion: 42 specific to resistant and 29 to susceptible individuals. Finally, 40 silicoDArT markers were suitable for primer design. From this pool, five markers, 3 for resistant and 2 for susceptible plants, were selected for product amplification in the expected groups. The developed method, based on 2 selection markers, provides certain identification of resistant and susceptible homozygotes. Also, the use of these markers allowed the determination of heterozygotes in the analysed population. Selected silicoDArT markers were also used for chromosomal localization of new resistance genes. Five out of 71 segregating silicoDArT markers for the Pm11 gene were found on the available consensus genetic map of oat. Five markers were placed on linkage groups corresponding to Mrg12 on the Avena sativa consensus map.

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Molecular mapping and identification of QTL's associated to oat crown rust partial resistance

Cited by 15 publications

References 31 publications

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Puccinia coronata f. sp. avenae: a threat to global oat production

Molecular identification and chromosomal localization of new powdery mildew resistance gene Pm11 in oat

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