Molecular level toxicological evaluation of a dental composite implanted in albino rats

Abstract

Dental composite (DC) resins are synthetic resins which are used as restorative material or adhesives in dentistry. Monomers and other components released into the oral environment from the DC material even after polymerization might affect the surrounding tissues and even the whole body gradually. Biocompatibility of DC has to be stringently evaluated since the fillings are in close contact with mucosa, tooth, and pulp. Common difficulties with various current dental materials include allergies, chemical leakage from the material, and pulpal irritation which are due to the byproducts of chemical reactions during different stages of material hardening. Dental Products Laboratory of Sree Chitra Tirunal Institute for Medical Sciences and Technology developed a new BIS-GMA-based DC. In this study the oxidative stress induced by this DC material in vivo was tested for a period of 1, 4, 8, 26, or 52 weeks in albino rats. Dental materials were implanted intramuscularly into the gluteus muscle and oxidative biomarkers such as lipid peroxidation, 8-oxo-deoxyguanosine (8-OHdG) concentration, and total glutathione content analyzed. Data showed no significant changes in antioxidant defense system and 8-OHdG levels in nuclear DNA of the tissue. Serum biochemical results confirmed that animals were in normal functioning physiological state.

Bioactive bacterial cellulose sulfate electrospun nanofibers for tissue engineering applications

“…It is reported to be involved in a crucial role of detoxification of xenobiotics, removal of hydroperoxides, and maintaining the oxidation state of protein sulfhydryls (Akerboom & Sies, ). When a cell is under oxidative stress, the level of GSH disulfide goes up due to the oxidation of GSH that may indicate pathological changes in the cells (Arun, Silja, Sheeja, Geetha, & Mohanan, ). Figure b,e shows the levels of GSH in L929 cells and liver homogenate after treating with different concentrations of BCS.…”