Molecular investigation of tick-borne pathogens in ticks from grazing cattle in Korea

Kang, Seung Won; Doan, Huong Thi Thanh; Choe, Se Eun; Noh, Jin Hyeong; Yoo, Mi Sun; Reddy, Kondreddy Eswar; Kim, Young Ha; Kweon, Chang-Hee; Jung, Suk-Chan; Chang, Ki Yoon

doi:10.1016/j.parint.2013.02.002

Cited by 44 publications

(41 citation statements)

References 38 publications

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“…world wide. In addition, Haemaphysalis longicornis has been documented to be infected by E. canis in Korea (Kang et al 2013). In this study, R. sanguineus s.l.…”

Section: Discussionmentioning

confidence: 63%

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Malik

Qamar

Ain

et al. 2018

Veterinary Medicine & Sci

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Canine monocytic ehrlichiosis is a tick-borne disease caused by an intracellular alpha-proteobacterium, Ehrlichia canis, which replicates within mononuclear cells in the host. This study was designed to use a polymerase chain reaction (PCR) protocol for the molecular detection of E. canis by the amplification of a portion of its 16S rRNA gene, as well as the effects of this alpha-proteobacterium on the haematological parameters of the sampled dogs and the risk factors associated with E. canis infection. A total of 151 blood samples were collected from dogs of various breeds at three sampling sites (Lahore, Rawalpindi/Islamabad and Multan) in Punjab, Pakistan. Data regarding the epidemiological factors (including age, gender, breed, body temperature, deworming, vaccination, mucous membrane status, hydration status, the presence of haematuria and tick infestation) were collected through a questionnaire at the time of sample collection. A 400 bp DNA fragment of the 16S rRNA gene of E. canis was amplified from 42 dog blood samples (28% of the total), [Lahore (N = 24), Rawalpindi/Islamabad (N = 13) and Multan (N = 05)] through PCR. Data analysis revealed that the character of the animals (age, sex and breed) had no significant association (P > 0.05) with the presence of E. canis. Various haematological parameters were also compared, and the results revealed that all of the parameters remained unaffected, except significantly lower white blood cell counts (P = 0.004) in E. canis-positive blood samples, as compared with the control group. We concluded that this is the first molecular confirmation of canine infection by E. canis using PCR. Moreover, no specific epidemiological parameter was found associated with the prevalence of E. canis in dogs.

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“…world wide. In addition, Haemaphysalis longicornis has been documented to be infected by E. canis in Korea (Kang et al 2013). In this study, R. sanguineus s.l.…”

Section: Discussionmentioning

confidence: 63%

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Malik

Qamar

Ain

et al. 2018

Veterinary Medicine & Sci

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“…muris-like agent (EMLA)' that has been used to describe this bacterium since its initial characterization. While this member of the genus Ehrlichia has only been detected in the upper midwestern USA, E. muris is considered to be an Old World pathogen, found in Eastern Europe and Japan [12][13][14][15][16][17]. Recently, genotypic, phenotypic and epidemiological comparisons of EMLA Wisconsin T , E. muris and other species of the genus Ehrlichia prompted us to re-examine our prior conclusion that Wisconsin T represented a novel species of the genus Ehrlichia.…”

Section: Wisconsinmentioning

confidence: 99%

“…E. muris has been found in Japan [17], Russia [14], Slovakia [12] and Korea [16], while EMLA strains, including Wisconsin T , have thus far been found only in Minnesota and Wisconsin in the Western Hemisphere [8][9][10]30]. Ixodes persulcatus and Haemaphysalis flava ticks serve as vectors of E. muris [12,31], whereas EMLA Wisconsin T is transmitted by I. scapularis [28,29,32].…”

Section: Emla Wisconsinmentioning

confidence: 99%

Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans

Pritt

Allerdice

Sloan

et al. 2017

International Journal of Systematic and Evolutionary Microbiology

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“…Several Korean studies have reported the presence of A. phagocytophilum in a variety of mammals, including rodents, dogs, and cattle (Chae et al 2008b;Jung et al 2012;Kang et al 2013), and a case of HGA has recently been documented in Korea (Kim et al 2014). In recent decades, there has been a gradual increase in temperatures worldwide due to global warming, and this climate change is expected to influence the abundance and distribution of ticks (Leger et al 2013).…”

mentioning

confidence: 99%

Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

Lee¹,

Kim²,

Yun³

et al. 2015

Vet. Med. - Czech

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ABSTRACT:The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.

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Molecular investigation of tick-borne pathogens in ticks from grazing cattle in Korea

Cited by 44 publications

References 38 publications

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans

Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

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