Molecular interaction studies of hemostasis: fibrinogen ligand–human platelet receptor interactions

Force measurements with the atomic force microscope: Technique, interpretation and applications

“…Host-guest complexes have played a predominant role in the study of specific forces. Several experiments have been performed with different complementary molecules in the last years [368,[1118][1119][1120][1121][1122][1123][1124][1125][1126][1127][1128][1129]. It also has been shown, that force-displacement curves with functionalized tips can be employed to distinguish molecules of different chirality [1130,1131].…”

A mechanobiological investigation of platelets

“…With this technique, one can collect force-distance curves for local surface locations, ultimately generating a force map image of the surface (77). Recent platelet investigations using this method looked into the local interactions of surface receptor GP Ilb-IIIa which has an important role in platelet adhesion, activation, and aggregation (78,79). The high-resolution capabilities of this method enabled direct visualization of single ligand/receptor interactions on platelets in a dynamic and physiologically relevant environment, proving the significance of this technique in future identifications of deformation-induced pathological thrombosis at the local level (76, 79).…”

Kinetics of swelling/shrinking rearrangement of a self-aggregated nanohydrogel at solid/liquid interfaces

“…Therefore, in this work, the swelling and shrinking along the layer thickness at solid/liquid interfaces were studied in terms of the length of hydrophilic MPC branches (n ¼ 0, 6.2, 14.3 and 180) of MC n . We report, for the first time, the kinetics of the swelling or shrinking controlled by the length of hydrophilic branch, using the advantages of AFM, which could deal with the biological soft samples under the physiological conditions [12,13] to visualise the dynamic changes of interface-induced changes of the MC n nanohydrogels.…”