Molecular Interaction between COP1 and HY5 Defines a Regulatory Switch for Light Control of Arabidopsis Development

Ang, Lay‐Hong; Chattopadhyay, Sudip; Wei, Ning; Oyama, Tokitaka; Okada, Kiyotaka; Batschauer, Alfred; Deng, Xing‐Wang

doi:10.1016/s1097-2765(00)80022-2

Cited by 605 publications

(648 citation statements)

References 47 publications

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“…The observation that the most dramatic morphological defects in the hy5 mutant are found in the hypocotyls, stems, and roots supports this conjecture (Ang and Deng, 1994;Oyama et al, 1997;Ang et al, 1998). Recently, LjBzf, a gene highly homologous to STF1, was isolated from L. japonicus (Nishimura et al, 2002).…”

Section: Discussionmentioning

confidence: 80%

“…One of the best characterized bZIP factors thought to play a role in photomorphogenic seedling development and hormone signaling in Arabidopsis (Arabidopsis thaliana) is LONG HYPOCOTYL5 (HY5) (Oyama et al, 1997;Ang et al, 1998;Holm et al, 2002;Cluis et al, 2004). The function of HY5 in photomorphogenesis is well illustrated in hy5 mutant seedlings, which have defects in light inhibition of hypocotyl elongation, in light-induced chlorophyll, and in anthocyanin accumulation (Oyama et al, 1997;Sibout et al, 2006;Shin et al, 2007).…”

mentioning

confidence: 99%

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DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins

et al. 2008

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LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was reported in soybean. To investigate the role of STF1, the phenotypes of transgenic Arabidopsis plants overexpressing STF1 and HY5 were compared. In addition, the DNA-binding properties of STF1 and HY5 were extensively studied using random binding site selection and electrophoretic mobility shift assay. Overexpression of STF1 in the hy5 mutant of Arabidopsis restored wild-type photomorphogenic and root development phenotypes of short hypocotyl, accumulation of chlorophyll, and root gravitropism with partial restoration of anthocyanin accumulation. This supports that STF1 is a homolog of HY5 with a role in light and hormone signaling. The DNA-binding properties of STF1 and HY5 are shown to be similar to each other in recognizing many ACGT-containing elements with a consensus sequence motif of 5#-( G / A )( G / A ) TGACGT( C /G/ A )( A /T/ G )-3#. The motif represents a characteristically strong preference for flanking sequence to TGACGT and a larger sequence than the sequences recognized by the G-box binding factor and TGA protein families. The finding of C-box, hybrid C/G-, and C/A-boxes as high-affinity binding sites over the G-box and parameters associated with HY5 recognition define the criteria of HY5/STF1 protein-DNA interaction in the promoter regions. This study helps to predict the precise in vivo binding sites of the HY5 protein from the vast number of putative HY5 genomic binding sites analyzed by chromatin immunoprecipitation on chip.

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Section: Discussionmentioning

confidence: 80%

mentioning

confidence: 99%

DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins

et al. 2008

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“…In this sense, the expression pattern and binding properties of STF1 observed in this report are consistent with the proposed function of STF1 in hypocotyl elongation. Recently the HY5 protein was reported to interact with the gene product of COP1, a constitutive photomorphogenic mutant that has short hypocotyls and expanded cotyledons (Ang et al, 1998). COP1 is a RING finger protein that has a zinc binding motif at its N-terminus, a putative coiled-coil-region, and several WD40 repeats with significant amino acid sequence similarity to a Drosophila TFIID subunit, TAFII80 (Deng et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

STF1 is a novel TGACG‐binding factor with a zinc‐finger motif and a bZIP domain which heterodimerizes with GBF proteins

et al. 1998

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SummaryTwo separate nuclear binding activities (B1 and B2) in the soybean apical hypocotyl have been identified that interact with a palindromic C-box sequence (TGACGTCA) and which are developmentally regulated in an inverse manner. The bZIP factors responsible for these two binding activities, B1 and B2, were isolated from a cDNA library and designated STGA1 and STFs (STF1 and STF2), respectively. Sequence analysis shows that the STFs contain both a zinc-finger domain and a bZIP domain. The two zinc finger sequences of Cys 4 -Cys 4 are most related to the RING zincfinger motif carrying a Cys 3 -His-Cys 4 . In addition the bZIP domain of STFs is highly homologous to the HY5 protein of Arabidopsis. DNA binding studies revealed that STF1 binding to the TGACGT sequence requires distinct flanking sequences. Furthermore, STF1 binds to the Hex sequence as a heterodimer with G-box binding factors (GBFs), a feature not observed with STGA1. Since STF1 expression is most prevalent in apical and elongating hypocotyls, it is proposed that STF1 may be a transcription factor involved in the process of hypocotyl elongation.

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“…7C). This is not the case if a control fusion protein between the Arabidopsis transcription factor HY5, which lacks transactivation potential (Ang et al 1998), and lexA is expressed instead. The transactivation potential is largely reduced in a truncated BRX protein comprising the 100 N-terminal amino acids.…”

Section: The Brx Protein Is Nuclear Localized and Can Activate Transcmentioning

confidence: 99%

Natural genetic variation in Arabidopsis identifies BREVIS RADIX, a novel regulator of cell proliferation and elongation in the root

Mouchel¹,

Briggs²,

Hardtke³

2004

Genes Dev.

226

237

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Mutant analysis has been tremendously successful in deciphering the genetics of plant development. However, less is known about the molecular basis of morphological variation within species, which is caused by naturally occurring alleles. In this study, we succeeded in isolating a novel regulator of root growth by exploiting natural genetic variation in the model plant Arabidopsis. Quantitative trait locus analysis of a cross between isogenized accessions revealed that a single locus is responsible for ∼80% of the variance of the observed difference in root length. This gene, named BREVIS RADIX (BRX), controls the extent of cell proliferation and elongation in the growth zone of the root tip. We isolated BRX by positional cloning. BRX is a member of a small group of highly conserved genes, the BRX gene family, which is only found in multicellular plants. Analyses of Arabidopsis single and double mutants suggest that BRX is the only gene of this family with a role in root development. The BRX protein is nuclear localized and activates transcription in a heterologous yeast system, indicating that BRX family proteins represent a novel class of transcription factors. Thus, we have identified a novel regulatory factor controlling quantitative aspects of root growth.

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Molecular Interaction between COP1 and HY5 Defines a Regulatory Switch for Light Control of Arabidopsis Development

Cited by 605 publications

References 47 publications

DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins

DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins

STF1 is a novel TGACG‐binding factor with a zinc‐finger motif and a bZIP domain which heterodimerizes with GBF proteins

Natural genetic variation in Arabidopsis identifies BREVIS RADIX, a novel regulator of cell proliferation and elongation in the root

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