Molecular insight into the immune up-regulatory properties of the leaf extract of Ashwagandha and identification of Th1 immunostimulatory chemical entity

Khan, Sheema; Malik, Fayaz; Suri, K. A.; Singh, Jaswant

doi:10.1016/j.vaccine.2009.07.011

Cited by 49 publications

(37 citation statements)

References 19 publications

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“…Withania somnifera also known as Ashwagandha is an Ayurvedic medicine which has been used for many decades for its anti-inflammatory, anti-oxidant [20], anti-stress and neuroprotection [61], immune boosting and memory power enhancing ability [70]. Raut et al [99] studied on W. somnifera to evaluate dose related tolerance, safety and activity and suggested that the average tolerance dose concentration was 750-1250 mg/day.…”

Section: Medicinal Plants and Natural Compounds Commonly Used For Neumentioning

confidence: 99%

Role of medicinal plants in neurodegenerative diseases

et al. 2017

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Neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by progressive loss (and even death) of structure and function of neurons, and have created great burden to the individual and the society. The actual cause of various neurodegenerative diseases still remains a mystery in healthcare. Some of the commonly studied environmental factors causes for neurodegenerative diseases are protein degradation, oxidative stress, inflammation, environmental factor, mitochondrial defects, familial history, and abnormal protein accumulation in neuron. However ageing plays a very important role in neurodegenerative diseases. Medicinal plants and natural compounds, such as Withania somnifera (ashwagandha), Ginseng, curcumin, resveratrol, Baccopa monnieri, Ginkgo biloba, and Wolfberry have been applied to prevent or alleviate neurological diseases and relief of neurological symptoms reported in in vivo or in clinical trails. Natural compounds in nanosize range as a therapeutic agent possess the same activity as in native state. Nanodrug delivery helps to increase the bioavailability of the drug and thereby specifically target cells and tissues. Nanoparticles, polymeric nanomicelles, complex polymers nanocrystal, and nanofibers are used to carry the medicinal plants for drug delivery system in the treatment of neurodegenerative diseases. Especially, electrospinning and electrospraying as straightforward yet versatile techniques for the production of nanosized fibers and particles possess huge potential in encapsulation of natural compounds for the neurodegenerative diseases. This review is a study to understand the role of nanotechnology and natural compounds in neurodegenerative diseases associated with ageing.

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Section: Medicinal Plants and Natural Compounds Commonly Used For Neumentioning

confidence: 99%

Role of medicinal plants in neurodegenerative diseases

et al. 2017

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“…Similarly, FITClabelled CD14 was added with PE conjugated anti-CD80 (B7-1) in one set, while FITC-labelled CD14 was added with PE-conjugated anti-CD86 (B7-2) monoclonal (mAbs) in another set for antigen presenting cells (APCs) (macrophages) after erythrocyte lysis with fluorescenceactivated cell sorting (FACS) lysis solution. Cells were then kept in the dark for 30 min at 4C, washed twice with PBS after staining, and subjected to FACS analysis 20 .…”

Section: Flow Cytometric Study Of Co-stimulatory Moleculesmentioning

confidence: 99%

Upregulation of Th1 Polarization by <i>Taraxacum officinale</i> in Normal and Immune Suppressed Mice

et al. 2016

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The present study was undertaken to study the potential of Taraxacum officinale aqueous extract (aerial part; TO-10) to maintain immune homeostasis in normal and chronic restraint stress and cyclosporine-A-induced immune suppressed mice. Immune restorative effect of test drug was evaluated first in normal and then in immune-compromised mice using flow cytometer and Elisa techniques. TO-10 enhanced the expression of T-cell subsets and CD28, CD69 and CD80/CD86 co-stimulatory molecules in sheep red blood corpuscles (SRBCs)-immunized mice. Flow cytometric analysis revealed that TO-10 upregulated the expression of Th1 cytokines, IL-2, IFN-gamma and IL-12, and regularized the increased expression of IL-10 in chronically stressed animals. It also normalized the elevated corticosterone levels, and reversed the chronic stress-induced hypertrophy of adrenal glands and atrophy of spleen and thymus. The results show that TO-10 is also able to maintain immune homeostasis in normal and immune-compromised conditions. Chicoric acid, a major constituent of TO-10, seems to be responsible for skewing to Th1 immune polarization as shown by its stimulatory effect on the expression of IFN-gamma and IL-2 in phorbol 12-myristate 13-acetate + ionomycin stimulated peripheral blood mononuclear cells (PBMCs).

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“…To quantify the expression of co-stimulatory molecules, macrophages were stained with FITC-labeled anti-CD80 (B7-1) and anti-CD86 (B7-2) mAbs on ice for 30 min and washed with PBS. Ten thousand cells were collected for each sample, and the data were analyzed with FACScan flow cytometer [15].…”

Section: Phenotypic Analysis Of Co-stimulatory Molecules (Cd80 and Cd86)mentioning

confidence: 99%

Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice

Sheikh

Khajuria

Parvaiz

et al. 2012

International Immunopharmacology

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Molecular insight into the immune up-regulatory properties of the leaf extract of Ashwagandha and identification of Th1 immunostimulatory chemical entity

Cited by 49 publications

References 19 publications

Role of medicinal plants in neurodegenerative diseases

Role of medicinal plants in neurodegenerative diseases

Upregulation of Th1 Polarization by <i>Taraxacum officinale</i> in Normal and Immune Suppressed Mice

Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice

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