Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice

Sheikh, Abid Ali; Khajuria, Anamika; Parvaiz, Qazi; Sidiq, Tabasum; Bhatia, Aruna; Singh, Surjeet; Ahmad, Shabir; Randhawa, Mansimran Kaur; Satti, Naresh K.; Dutt, Prabhu

doi:10.1016/j.intimp.2012.02.001

Cited by 26 publications

(11 citation statements)

References 30 publications

(33 reference statements)

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“…The recent and previous studies showed that both CD4+ and CD8+ T-cells were able to control immune response by the regulation of cytokines. According to a recent research, the CD4+ T-cells differentiate into one of the two effector phenotypes where Th1 cells drive the immune response toward a cell-mediated immune response, while Th2 cells stimulate a humoral or allergic response 37,38. The augmentation of B- and T-cells with TCE might be due to the Th1/Th2 cytokine-mediated mechanism.…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Ahmad

Jantan

Kumolosasi

et al. 2015

DDDT

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Tinospora crispa (TC) has been used in folkloric medicine for the treatment of various diseases and has been reported for several pharmacological activities. However, the effects of TC extract on the immune system are largely unknown. Therefore, the present study was aimed to investigate the immunomodulatory effects of a standardized 80% ethanol extract of the stem of TC on innate immune responses. Male Wistar Kyoto rats were treated daily at 100 mg/kg, 200 mg/kg, and 400 mg/kg doses of the extract for 21 days by oral gavage. The immunomodulatory potential of TC was evaluated by determining its effect on chemotaxis and phagocytic activity of neutrophils isolated from the blood of rats. To further elucidate the mechanism of action, its effects on the proliferation of T- and B-lymphocytes and T-lymphocytes subsets (CD4+ and CD8+) and on the secretion of Th1 and Th2 cytokines were also monitored. The main components of the extracts, syringin and magnoflorine, were identified and quantitatively analyzed in the extracts by using a validated reversed-phase high-performance liquid chromatography method. It was observed that the chemotactic activity of neutrophils obtained from extract-treated rats increased as compared to controls. A dose-dependent increase in the number of migrated cells and phagocytosis activity of neutrophils was observed. Dose-dependent increase was also observed in the T- and B-lymphocytes proliferation stimulated with concanavalin A (5 μg/mL) and lipopolysaccharide (10 μg/mL), and was statistically significant at 400 mg/kg (P>0.01). Apart from cell-mediated immune response, the concentrations of Th1 (TNF-α, IL-2, and IFN-γ) and Th2 (IL-4) cytokines were significantly increased in sera of rats treated with different doses as compared with the control group. From these findings, it can be concluded that TC possesses immunostimulatory activity and has therapeutic potential for the prevention of immune diseases.

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Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Ahmad

Jantan

Kumolosasi

et al. 2015

DDDT

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“…An increase in natural killer cytotoxicity has also been noted in this latter study. In animal studies, mice fed a bioactive ethyl acetate fraction derived from Prunus cerasus (sour cherry) had increased proliferative responses of both B- and T-lymphocytes, and increased IgM and IgG production from B-lymphocytes ( 59 ) .…”

Section: Discussionmentioning

confidence: 99%

Dietary strawberries increase the proliferative response of CD3/CD28-activated CD8⁺ T cells and the production of TNF-α in lipopolysaccharide-stimulated monocytes from obese human subjects

et al. 2013

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Obesity increases the risk of developing bacterial and viral infections compared with normal weight. In a 7-week double-blind, randomised, cross-over trial, twenty obese volunteers (BMI between 30 and 40 kg/m 2 ) were fed freeze-dried strawberry powder or strawberryflavoured placebo preparations to determine the effects of dietary strawberries on immune function. Blood was collected at six time points during the study and peripheral blood mononuclear cells (PBMC) were isolated at each time point and activated with CD3 plus CD28 antibodies (T-lymphocyte activation) or lipopolysaccharide (LPS, monocyte activation). Interferon-g, TNF-a, IL-4 and IL-10 were measured in supernatants from the activated T cells. Supernatants from the activated monocytes were analysed for the production of TNF-a, IL-1b, IL-6 and IL-8. PBMC were pre-stained with PKH (Paul Karl Horan) dye and activated with CD3 plus CD28 antibodies to determine the proliferative responses of CD4 þ and CD8 þ T-lymphocytes by flow cytometry. To detect global changes in gene expression, microarray analysis was performed on LPS-and vehicle-treated PBMC from two subjects before and after the strawberry intervention. No difference was observed for the production of T-cell cytokines between the intervention groups. The production of TNF-a was increased in the supernatants from LPS-activated PBMC in the group consuming strawberries compared with the placebo. A modest increase in the proliferation of the CD8 þ T-lymphocyte population was observed at 24 h post-activation. These data suggest that dietary strawberries may increase the immunological response of T-lymphocytes and monocytes in obese people who are at greater risk for developing infections.

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“…In this study, immune activities of Mao-tofu and the two extracts were explored via assessing their effects on serum contents of three immunoglobulins and two complements. Food components with immune activities can stimulate the production of immunoglobulins; for example, the bioactive fraction from Prunus cerasus can increase both serum IgG and IgM contents in the BALB/c mice (Abid et al, 2012). Tryptic hydrolysates from Labeo rohita roe are able to increase serum IgA in the model mice (Chalamaiah et al, 2014).…”

Section: Serum Immunoglobulins and Complements Of The Mice Responsiblmentioning

confidence: 99%

Immune potentials of theMucor-fermented Mao-tofu and especially its soluble extracts for the normal mice

Liu

Zhao

2017

Food and Agricultural Immunology

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Mao-tofu was fermented for 6 days with a Mucor strain and extracted with pH 6.5 water and 40% ethanol to generate respective two extracts. Immune potentials of Mao-tofu and two extracts were assessed, via assessing the changes of several immune indices related to immune organs, cells, and molecules in normal mice. Compared with the unfermented tofu, Mao-tofu had somewhat higher immune activities towards the mice in all cases, indicating improved immune potential by the Mucor-mediated fermentation. More important, two extracts (especially water extract) had much higher immune activities towards the mice than Mao-tofu (P < .05). Two extracts were in dose-dependent manner to improve immune status of the mice, via increasing spleen and thymus indices, percentages of T-helper (CD4+) and T-cytotoxic (CD8+) lymphocytes in plasma, serum contents of two complements (C3 and C4) and Th1 cytokines (IL-2 and IFN-γ), but decreasing serum contents of Th2 cytokines (IL-4, IL-5, and IL-10).

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Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice

Cited by 26 publications

References 30 publications

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Dietary strawberries increase the proliferative response of CD3/CD28-activated CD8⁺ T cells and the production of TNF-α in lipopolysaccharide-stimulated monocytes from obese human subjects

Immune potentials of theMucor-fermented Mao-tofu and especially its soluble extracts for the normal mice

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Immunomodulatory studies of a bioactive fraction from the fruit of Prunus cerasus in BALB/c mice

Cited by 26 publications

References 30 publications

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats

Dietary strawberries increase the proliferative response of CD3/CD28-activated CD8+ T cells and the production of TNF-α in lipopolysaccharide-stimulated monocytes from obese human subjects

Immune potentials of theMucor-fermented Mao-tofu and especially its soluble extracts for the normal mice

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Dietary strawberries increase the proliferative response of CD3/CD28-activated CD8⁺ T cells and the production of TNF-α in lipopolysaccharide-stimulated monocytes from obese human subjects