Molecular identification of vectors of Leishmania in Colombia: Mitochondrial introgression in the Lutzomyia townsendi series

Testa, J. M.; Lerma, James Montoya; Cadena, Horacio; Oviedo, Milagros; Ready, P. D.

doi:10.1016/s0001-706x(02)00187-0

Cited by 55 publications

(54 citation statements)

References 17 publications

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“…Therefore, the method established in this study would be useful for epidemiological surveillance of leishmaniasis and its vector, Lutzomyia species. Alternatively, additional analyses targeting other genes, such as 12S and 28S rRNA genes, the mitochondrial cytochrome b gene, and internal transcribed spacer 2 (ITS2) regions, promise reliable information [3,6,7,10,[18][19][20]22].…”

Section: Discussionmentioning

confidence: 99%

Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

Terayama

Kato

Gómez³

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Surveillance of the distribution of sand fly species is important for prediction of the risk and expansion of Leishmania infection in endemic and surrounding areas. In the present study, a simple and reliable method of typing New World Lutzomyia species circulating in endemic areas in Ecuador was established by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique. PCR-RFLP of 18S ribosomal RNA (rRNA) genes with the restriction enzyme AfaI and subsequently HinfI successfully identified seven sand fly species in nine endemic areas in Ecuador. Although intraspecific genetic-diversity affecting the RFLPpatterns was detected in a species, the patterns were species specific. The method promises to be a powerful tool for the classification of New World Lutzomyia species.

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Section: Discussionmentioning

confidence: 99%

Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

Terayama

Kato

Gómez³

et al. 2008

The Journal of Veterinary Medical Science

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“…Sandflies were identified based on external and internal morphological characters of the head and genitalia (Léger et al, 1983;Gil Collado et al, 1989;Benabdennbi et al, 1999;Pesson et al, 2004), which were slide-mounted in Berlese fluid or Marc André solution following dissection with sterilized forceps and microneedles. Dissections were carried out in a room distant from the molecular biology laboratory, following the protocols of Testa et al (2002) to reduce the risk of Polymerase Chain Reaction (PCR) carryover. The dissected thorax and the anterior abdomen were used, separately or combined, to extract proteins or total DNA.…”

Section: Collection and Identification Of Specimensmentioning

confidence: 99%

Postglacial dispersal ofPhlebotomus perniciosusinto France

et al. 2005

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Summary :Phlebotomus perniciosus was identified morphologically in samples from France and northeast Spain, and individuals were then characterized at three polymorphic isoenzyme loci (by isoelectrofocusing) and at the mitochondrial DNA locus (by comparative DNA sequence analysis of a fragment of the Cytochrome b gene). The four polymorphic loci gave conflicting patterns of population relationships, which can be explained by hypothesizing different amounts of gene introgression at each locus when two distinctive lineages met in southern France or northeast Spain after isolation in southern Italy and Spain during the Pleistocene Ice Ages. P. perniciosus is an important vector of Leishmania infantum and so these population differentiation studies are relevant for predicting the emergence and spread of leishmaniasis in relation to environmental changes, including climate. Résumé

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“…Éste se compone de una región control, 13 genes codificadores de proteínas, dos genes que codifican para las subunidades ribosomales y 22 genes para ARN de transferencia (ARNt) (10). En estudios taxonómicos, tanto interespecíficos como intraespecíficos del género Lutzomyia França, 1924, se han usado los genes citocromo b, citocromo oxidasa I, NAD deshidrogenasa 1 y 4 y la subunidad pequeña ribosomal (11)(12)(13)(14)(15)(16)(17), que en conjunto representan sólo el 13% de la diversidad de genes de la molécula mitocondrial.…”

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Análisis de la estructura primaria y secundaria del ARN de transferencia mitocondrial para serina en siete especies de Lutzomyia

2007

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Introducción. Los insectos del género Lutzomyia son los responsables de la transmisión del parásito Leishmania spp. en América. La taxonomía de estos vectores se fundamenta en los caracteres morfológicos que exhiben los adultos, principalmente, en las estructuras pareadas de la cabeza y los genitales. Aunque estos caracteres permiten distinguir la mayoría de los taxones, la similitud en algunos subgéneros y grupos de especies pone límites a la identificación por criterios morfológicos. Objetivo. Evaluar la utilidad del ARN de transferencia mitocondrial para serina ARNt Ser en la determinación taxonómica de Lutzomyia. Materiales y métodos. Se analizaron siete especies flebotomíneas, L. trinidadensis, L. panamensis, L. cayennensis cayennensis, L. dubitans, L. gomezi, L. rangeliana y L. evansi. A partir de cada individuo, se extrajo, amplificó y obtuvo la secuencia del gen mitocondrial que codifica para el ARNt Ser , delimitado por los genes citocromo b y NAD deshidrogenasa uno. La estructura secundaria del ARNt Ser se infirió teniendo como base las estructuras homólogas descritas en otros insectos del orden Diptera. Resultados. La longitud del gen ARNt Ser osciló entre 66 pb en L. gomezi y 69 pb en L. trinidadensis. En el alineamiento nucleotídico de 70 posiciones, se detectaron 14 sitios polimórficos, incluyendo cuatro eventos indel. La mayoría de las sustituciones correspondieron a las lupas dihidrouridina, ribotimidina-pseudouridina-citosina y variable, así como al extremo basal del brazo anticodón. Conclusión. Los cambios en la secuencia primaria de nucleótidos y los rearreglos en la estructura secundaria del ARNt Ser son potencialmente útiles para la discriminación taxonómica de las especies flebotomíneas estudiadas.Palabras clave: Psychodidae/clasificación, ARN de transferencia/genética, mitocondria, ADN, leishmaniasis.Analysis of the primary and secondary structure of the mitochondrial serine transfer RNA in seven species of Lutzomyia Introduction. Lutzomyia sand flies are involved in the transmission of the parasite Leishmania spp. in America. The taxonomy of these vectors is traditionally based on morphological features of the adult stage, particularly the paired structures of the head and genitalia. Although these characters are useful to distinguish most species of Lutzomyia, morphological identification may be complicated by the similarities within subgenera and species group. Objective. To evaluate the utility of mitochondrial serine transfer RNA tRNA Ser for taxonomic identification of Lutzomyia. Materials and methods. Seven sand fly species, each representing one of the 27 taxonomic subdivisions in genus Lutzomyia, were analyzed including L. trinidadensis (Oswaldoi group), L. (Psychodopygus) panamensis, L.(Micropygomyia) cayennensis cayennensis, L. dubitans (Migonei group), L. (Lutzomyia) gomezi, L. rangeliana (ungrouped) and L. evansi (Verrucarum group). The mitochondrial tRNA Ser gene, flanked by the cytochrome b and NAD dehydrogenase subunit one genes, was extracted, amplified and sequenced from ...

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Molecular identification of vectors of Leishmania in Colombia: Mitochondrial introgression in the Lutzomyia townsendi series

Cited by 55 publications

References 17 publications

Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

Postglacial dispersal ofPhlebotomus perniciosusinto France

Análisis de la estructura primaria y secundaria del ARN de transferencia mitocondrial para serina en siete especies de Lutzomyia

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