Molecular identification of unilocular hydatid cysts from domestic ungulates in Ethiopia: Implications for human infections

Hailemariam, Zerihun; Nakao, Minoru; Menkir, Sissay; Lavikainen, Antti; Yanagida, Tetsuya; Okamoto, Masanori; Itō, Akira

doi:10.1016/j.parint.2012.01.007

Cited by 39 publications

(27 citation statements)

References 18 publications

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“…These sequence showed 100% identity to published sequences of G6 derived from different hosts such as AB208063 from camels in Kazakhstan [9], AB458677 from goats in Peru [30], and AB688142 from humans in Russia (direct submission). Only one sequence (35420) had a single nucleotide substitution of C to T at position of 327 of the generated sequences, but was identical to sequence AB650535 generated from camels in Ethiopia [31]. The sequence of G5 genotype showed 99% identity with a single nucleotide substitution of C to T comparing to reference sequences (FJ744757, AB235846 and JX854035) at position 52.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly in Libya, G1 is the exclusive genotype in humans with some occurrence in cattle, whereas G6 dominates in cattle and camels [16]. G1 is also the common genotype in different hosts in Ethiopia [31], Tunisia [39], Palestine [41], Iran [42], India [43], China [44] and Mongolia [45]. Echinococcus granulosus s.s. (G1- G3 complex) is also the major genotype in humans, cattle, sheep and goats in many European and Latin American countries [27,46,47].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

et al. 2015

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Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

et al. 2015

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“…Sudanese sheep have been found to be infected by E. canadensis G6-G7 genotypes only, strains that are typically more adapted to pigs, camels and cervids. As expected, E. granulosus s.s. is better adapted to sheep than E. canadensis, with the former species inducing cyst fertility rates ranging from 37% to 67% (Hailemariam et al, 2012;M'Rad et al, 2005) compared to those of 19-39% achieved by the later species (Ibrahim et al, 2011;Omer et al, 2010). Molecular genotyping studies in goats are restricted so far to Kenya and Sudan.…”

Section: Molecular Epidemiology Of Cystic Echinococcosis In Productiomentioning

confidence: 99%

“…Although preliminary studies have suggested that most of the hydatid cysts in cattle are sterile (Thompson et al, 1984), this seems to be not always the case. Thus, cyst fertility rates ranging from 0% to 70% have been reported in cattle infected by E. granulosus s.s. (including the G1 variant) and E. canadensis (Addy et al, in press;Hailemariam et al, 2012;Ibrahim et al, 2011;Omer et al, 2010;M'Rad et al, 2005), suggesting that African cattle can act as suitable hosts for both species in some areas. Unfortunately cyst fertility data are not currently available for E. ortleppi infections in Africa.…”

Section: Molecular Epidemiology Of Cystic Echinococcosis In Productiomentioning

confidence: 99%

A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals

Cardona

Carmena

2013

Veterinary Parasitology

193

143

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“…Previous investigations show that cystic echinococcosis is one of the major causes of organ condemnation in the country (Kumsa 1994;Kebebe et al 2010, Kumsa andMohammedzein 2012). Cystic echinococcosis in cattle has been reported from some parts of the country (Kumsa 1994;Hailemariam et al 2012). However, there is lack of information on cystic echinococcosis of cattle in Gondar and its Surroundings.…”

Section: Introductionmentioning

confidence: 99%

Cystic echinococcosis in cattle slaughtered at Gondar Elfora export Abattoir, northwest Ethiopia

2013

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A cross sectional and retrospective studies were conducted from November 2010 to April 2011 to determine the prevalence and characteristics of hydatid cysts in cattle slaughtered at Gondar Elfora Abattoir in northern Ethiopia. Out of the 308 cattle examined for the presence of hydatid cysts, 63 (20.5 %) of them were found harboring hydatid cysts in one or more of their internal organs. Results of the study showed higher (P \ 0.05) prevalence of cystic echinococcosis in adult (22.4 %; 49/219) than in young (15.7 %; 14/89) cattle. Lowest prevalence of hydatid cysts was observed in cattle from Debark (7.7 %; 2/26) whereas highest prevalence of cystic echinococcosis was recorded in cattle from Fogera (28.2 %; 11/39). The lungs (69.3 %; 61/88) were the most affected organs followed by the livers (28.4 %; 25/88). A total of 230 hydatid cysts from different internal organs of 63 infected cattle were differentiated into 60.4 % calcified, 30 % fertile and 9.6 % sterile cysts. Likewise, a total of 13 % (30/230) small, 15.6 % (36/230) medium, and 10.9 % (25/230) large hydatid cysts were recorded. Lung harbored the highest overall count (76.9 %; 177/230) and greatest proportion of large size cysts (13.5 %; 103/177) than all the other organs. Liver harbored the highest proportion of calcified cysts (68 %; 34/50). An overall proportion of 30 % (69/230) fertile cysts were recorded. The greatest proportion of fertile cysts (33.3 %; 59/177) was recorded in lungs followed by the livers (20 %; 10/50). A retrospective data of 5 years (2004/5-2009/10) revealed an overall prevalence of 30.4 % (9,106/29,951) and highest overall prevalence of 65.5 % hydatid cysts in the lungs followed by livers (33.5 %) and least in spleen (0.04 %). In conclusion the findings reported herein show that cystic echinococcosis is widespread in cattle slaughtered in Gondar export Abattoir and suggests that the lung is the most important source of hydatid cysts for definitive hosts in the area.

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Molecular identification of unilocular hydatid cysts from domestic ungulates in Ethiopia: Implications for human infections

Cited by 39 publications

References 18 publications

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals

Cystic echinococcosis in cattle slaughtered at Gondar Elfora export Abattoir, northwest Ethiopia

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