Molecular identification of two species of the carnivorous sea slug Philine, invaders of the US west coast

Krug, Patrick J.; Asif, Jamal H.; Baeza, Isabel; Morley, Margaret S.; Blom, Wilma M.; Gosliner, T. M.

doi:10.1007/s10530-012-0242-9

Cited by 10 publications

(9 citation statements)

References 55 publications

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“…The determination of the identity of non‐indigenous invasive species is fundamental to assess their origin, and hence to understand the characteristics that have facilitated their colonization and establishment success, as well as to allow their potential control or eradication (Stepien & Tumeo, ; Dubey & Shine, ; Krug et al ., ). However, the correct identification of alien species is not always evident, particularly when it is based on morphological characteristics.…”

Section: Introductionmentioning

confidence: 97%

Multiple origins of invasive and ‘native’ water frogs (Pelophylaxspp.) in Switzerland

Dubey

Leuenberger

Perrin

2014

Biol J Linn Soc Lond

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The marsh frog (Pelophylax ridibundus) has been introduced in many areas in Central and Western Europe as a result of commercial trade with Eastern Europe, and is rapidly replacing the native pool frog (P. lessonae). A large number of Pelophylax species are distributed in Eastern Europe and the strong phenotypic similarity between these species is rendering their identification hazardous. Consequently, alien populations of Pelophylax might not strictly be composed of P. ridibundus as previously suspected. In the present study, we analysed the cytochrome-b and NADH dehydrogenase subunit 3 genes of introduced and native Pelophylax species from Switzerland (299 individuals) in order to properly identify the source populations of the invaders and the genetic status of the native species. Our study highlighted the occurrence of several genetic lineages of invasive frogs in western Switzerland. Unexpectedly, we also showed that several populations of the native pool frog (P. lessonae) cluster with the Italian pool frog P. bergeri from central Italy (considered by some authors as a subspecies of P. lessonae). Hence, these populations are probably also the result of introductions, meaning that the number of native P. lessonae populations is fewer than expected in Switzerland. These findings have important implications concerning the conservation of the endemic pool frog populations, as the presence of multiple alien species could strongly affect their long-term subsistence.

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Section: Introductionmentioning

confidence: 97%

Multiple origins of invasive and ‘native’ water frogs (Pelophylaxspp.) in Switzerland

Dubey

Leuenberger

Perrin

2014

Biol J Linn Soc Lond

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“… Philine argentata was maintained as a distinct species by Chaban (2014) owing to differences in gizzard plate morphology, and subsequently by Chaban et al (2019) . The Chaban et al (2019) sequence of P. scalpta A. Adams, 1862 ( MN326894 ) was unexpectedly included with robust bootstrap support in a clade of sequences supposed to be from P. orientalis that were collected by Krug et al (2012) .…”

Section: Resultsmentioning

confidence: 99%

“…We determined sequences of 16S ribosomal RNA ( 16S rRNA ), histone H3 ( H3 ), and the D1 expansion region of 28S ribosomal RNA (D1 28S rRNA ). We then performed phylogenetic analyses of the sequences, including sequences from previous DNA studies of the Philinidae ( Krug et al 2012 ; Ohnheiser and Malaquias 2013 ; Gonzales and Gosliner 2014 ; Oskars et al 2015 ; Chaban et al 2019 ). We were unable to sequence the cytochrome c oxidase subunit I bar-coding region, a difficulty also found by Krug et al (2012) , possibly explaining why there are relatively few GenBank accessions for this gene from Philine .…”

Section: Introductionmentioning

confidence: 99%

Rare specimen identification in an un-integrated taxonomy: implications of DNA sequences from a Taiwanese Philine (Mollusca, Philinidae)

Colgan¹,

Ahyong²,

Mardon³

et al. 2021

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Many species of the gastropod genus Philine have been named from northeastern Asia but scanty descriptions based predominantly on shells make it difficult to determine which are valid. This, plus the sporadic anatomical and genetic information available for many of these species has led to what may be described as an un-integrated taxonomy. In this situation, it is generally preferable to postpone dissection of rare and unusual specimens until relevant diagnostic characters can be established in broader studies. Micro-CT scanning and DNA sequencing were used to examine such a specimen collected recently from deep waters off northeastern Taiwan. Micro-CT examination of the morphology of the internal shell and gizzard plates suggested that, among named species, the sequenced specimen is most similar to P. otukai. It cannot, however, be definitively referred to P. otukai as that species lacks adequate anatomical description or known DNA sequences. Phylogenetic analyses of newly collected DNA sequences show the specimen to be most closely related to, but distinct from the northern Atlantic Ocean and Mediterranean species, Philine quadripartita. The sequences also confirm genetically that five or more species of Philine occur in northeast Asia, including at least three subject to considerable taxonomic uncertainty.

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“…Contrary to the H3 results, all specimens of D. parguerensis from two geographically distant locations (Puerto Rico and Panama) have unique substitutions in the 16S gene (genetic synapomorphies) that suggest reproductive isolation from D. occidentalis . The 16S gene is variable enough to distinguish among species of opisthobranchs of diverse clades (Turner & Wilson, 2007; Anthes et al , 2008; Johnson & Gosliner, 2012, Krug et al , 2012a) and is even informative in population genetics studies (Krug et al , 2012b). Moreover, the F ST analysis of 16S confirmed that the two species ( D. parguerensis and D. occidentalis ) are genetically distinct.…”

Section: Discussionmentioning

confidence: 99%

Molecular divergence between two sympatric species ofDondice(Mollusca: Nudibranchia) with distinct feeding specializations

2013

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Analysis of mitochondrial (16S) and nuclear (H3) gene data using phylogenetic and population genetic approaches has revealed some genetic differences between two putative species of western Atlantic Dondice opisthobranchs that feed differentially on hydroids or on up-side-down jellies of the genus Cassiopeia. These results partially support the validity of the species Dondice parguerensis, which was described for the jelly-eating Dondice. However, phylogenetic analyses revealed that the hydroid-feeding species Dondice occidentalis and D. parguerensis are not reciprocally monophyletic and they are identical for the nuclear H3 gene. Although there are morphological and developmental differences between these two nominal species, the molecular data are inconclusive. A possible explanation is that the two putative species are in the process of speciation due to different feeding habits, resulting in the presence of genetic synapomorphies in D. parguerensis, but only in the more variable 16S gene. Because the ranges D. occidentalis and D. parguerensis overlap and there are no obvious barriers to gene flow between the two putative species, this may constitute a possible example of incipient sympatric speciation in benthic marine organisms.

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Molecular identification of two species of the carnivorous sea slug Philine, invaders of the US west coast

Cited by 10 publications

References 55 publications

Multiple origins of invasive and ‘native’ water frogs (Pelophylaxspp.) in Switzerland

Multiple origins of invasive and ‘native’ water frogs (Pelophylaxspp.) in Switzerland

Rare specimen identification in an un-integrated taxonomy: implications of DNA sequences from a Taiwanese Philine (Mollusca, Philinidae)

Molecular divergence between two sympatric species ofDondice(Mollusca: Nudibranchia) with distinct feeding specializations

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