Molecular identification of Tribolium castaneum and T. confusum (Coleoptera: Tenebrionidae) using PCR-RFLP analysis

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“…The restriction analysis of PCR-amplified fragments using restriction endonucleases (RFLP-PCR) is a practical alternative to gene sequencing for determining differences in gene sequences, as it is a low cost, easy, and fast method (Ming et al, 2014). The mitochondrial COI gene is used most frequently for DNA-based species identification (Soroka, 2008).…”

“…Besides protein-based methods, more reliable DNAbased methods are also being used (Agerberg, 1990;Fevolden et al, 1994). Single nucleotide polymorphism (SNP) (Akhan et al, 2014), amplified fragment length polymorphism (AFLP) (Chiesa et al, 2011), restricted fragment length polymorphism (RFLP) Grandjean et al, 1997;Gouin et al, 2003) and single sequence repeat (SSR) Yue et al, 2010;Gouin et al, 2011;Iorgu et al, 2011, Vorburger et al, 2014Ming et al, 2014) are some of the very useful methods that have been used for analyzing both population genetics and systematic classification of crayfish. These DNA-based methods provide rapid and accurate results that facilitate species identification and distinction between closely related species and subspecies of fish, crayfish and other organisms.…”

“…These DNA-based methods provide rapid and accurate results that facilitate species identification and distinction between closely related species and subspecies of fish, crayfish and other organisms. The PCR-RFLP method is based on the restriction digestion of DNA amplified from highly conserved regions, such as the mitochondrial genome (Ming et al, 2014). This method is easier, more practical and cheaper than other molecular tools used for species identification, such as microsatellites, sequence analysis and molecular barcoding and it has been successfully used for the identification of crayfish species (Soroka, 2008), fish species (Teletchea, 2009;Sumathi et al, 2015), shrimp species (Khamnamtong et al, 2005;Pascoal et al, 2011), and other aquatic organisms (Chow et al, 2006;Guerao et al, 2011;Jagadeesan et al, 2009).…”

Discriminación multivariante entre machos de Tribolium confusum Duval y Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) usando morfometría antenal

“…Para el control de las poblaciones de estas especies de insectos plaga en los sitios de almacenamiento, la aplicación de insecticidas de origen químico constituye en la actualidad la herramienta mayormente implementada. Ante el uso inadecuado e indiscriminado de estos agentes químicos, las poblaciones de T. confusum y T. castaneum han desarrollado resistencia hacia varias clases de plaguicidas que se usan contra ellas; sin embargo, dicha resistencia es diferencial tanto interespecífica como intraespecífica (estadios inmaduros y adultos) (Zettler 1991;Zhu et al 2010;Ming et al 2014). Además, se debe tener en consideración la toxicidad potencial de estos productos químicos tanto para el ser humano como para los demás seres vivos (Cassereau et al 2017).…”

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“…T. confusum and T. castaneum were sibling species, as they have almost same morphological characters and close genetic distance (Ming et al, 2014). It was strange that no Wolbachia infection T. castaneum strain was found in the wild, neither in the published scientific reports until now (Goodacre et al, 2015;Kageyama et al, 2010;Li et al, 2015).…”