Untitled

Abstract

Background: Increasingly interests in the potential use of insect symbionts Wolbachia to control populations of pest species were focused on many vector pest species and agricultural insects. However, few pieces of researches were reported in stored product insects. Methods: We surveyed the prevalence of Wolbachia using a PCR detection method in 10 Tenebrionidae stored product insects. Subsequently, Wolbachia infection density and spatiotemporal dynamics in Tribolium confusum were investigated in detail by TaqMan probe real-time quantitative PCR, and Wolbachia elimination patterns by antibiotic treatment and host reproductive fitness parameters were compared.