Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: polymerase chain reaction

Abstract

Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)—polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that Termitomyces aurantiacus (3), Tricholoma matsutake (8), Tricholoma robustum (2), P. ostreatus (4), Schizophyllum commune (1) and Pleurotus pulmonarius (1) were fully represented. Only Tricholoma matsutake (KT273371), Pleurotus pulmonarius (KY962469) and Tricholoma matsutake (AF438605) had 100% similarity with reference strain. However, the phylogenetic analysis of the isolates showed low genetic relatedness with reference strains. This study revealed the novelty of the mushroom strains and thus advocating the need for strict conservation measures and further investigations on their potential benefits to mankind.

Molecular characterization of most cultivated Pleurotus species in sub-western region Nigeria with development of cost effective cultivation protocol on palm oil waste

“…It is clearly shown that there are overlapping in characterizing Pleurotus species using morphological features, which needed to be corrected by using molecular features. The current work corroborates Adeniyi et al. (2018) who ascertained the identity of P. ostreatus and P. pulmonarius from Ilesa, Southwest region Nigeria with 93 and 100% similarity.…”

“…The result from the Phylogenetic analysis clearly shown that six of the collected samples falls under the species P. ostreatus while only two are P. pulmonarius species. Phylogenetic analysis of the samples showed high genetic relatedness with reference organisms, this is indication that mushroom samples are similar from the reference sequences from the Genbank ( Adeniyi et al., 2018 ). Non cluster or low genetic relatedness of the samples with the reference taxa from the GenBank database revealed distinct nucleotide signature different from existing organisms in the database ( Togashi et al., 2001 ; Aremu and Babalola 2015 ).…”

“…(2013) reported the isolation and characterization of P. pulmonarius (LAU09 - JF736658) and P. ostreatus (LAU 10- JF736659) from a wild basidiocarp growing on dead palm oil tree inside a Nigerian cocoa plantation in Ogbomoso area (347 m altitude) which also located in the Southwest Nigeria. P. pulmonarius (MF037415) and P. ostreatus (MF037419) were isolated on Dead Mangifera indica log and Dead Elaeis guineensis log respectively in Environmental Pollution Science and Technology (ENPOST) farm, Ilesa, Southwest Nigeria which located between (Longitude 4°42′30″E to 4°42′45″E and Latitude 7°36′55″N to 7°37′10″N) ( Adeniyi et al., 2018 ). The characterization of P. ostreatus and P. pulmonarius in this study will resolve the problem of inaccuracy in identification in both species among mushroom farmers in Southwest Nigeria.…”

Cultivation and Nutritional Value of Prominent Pleurotus spp.: An Overview

“…In recent decades, various biochemical and molecular techniques have been employed to investigate the phylogenetic relationships and the taxonomical hierarchy [34]. The DNA based molecular identification techniques such as internal transcribed spacer region identification, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP) has been widely used to validate Pleurotus species [35][36][37][38]. Through these genetic diversity studies, the ambiguous taxonomy of Pleurotus species may be employed in clarifying the misidentified species and strains in the literature.…”

Molecular identification and optimization of cultural conditions for mycelial biomass production of wild strain of Chlorophyllum molybdites (G.Mey) Massee from the Philippines

“…In addition, Reyes et al [8] reported the molecular identity of two newly recorded Termitomyces species in the Philippines, the T. bulborhizus and T. clypeatus with similarities of 92% and 87%, respectively. Moreover, Adeniyi et al [22] confirmed the identity of Termitomyces aurantiacus, Tricholoma matsutake, Tricholoma robustum, P. ostreatus, Schizophyllum commune, and Pleurotus pulmonarius with similarities between 77% and 100. Nevertheless, in the phylogenetic study of Vellinga et al [23], Chlorophyllum was found to belong within Agaricaceae.…”