Molecular Identification of Phenol-Degrading and Biofi lm-Forming Bacteria from Wastewater and Peat Soil

Abstract

Phenol is hazardous aromatic pollutant which needs to be treated to reduce its hazardous effects. Bioremediation using bacteria which can form biofi lm offer an alternative wastewater treatment that is cheaper and environmentally safe. Eighteen strains of phenol-degrading and biofi lm-forming bacteria were isolated from peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates were performed using Folin-ciocalteau reagent, while for biofi lm formation ability were performed using microtiter plate and crystal violet dye. Based on the ability to degrade phenol and to form biofi lm, four isolates (HP3, DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as biofi lm-forming bacteria. Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strain DSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested that DL120 and ATA6 could be classifi ed to the genus of Micrococcus and Bacillus respectively.

Identification of hydrocarbon-degrading bacterial consortium isolated from the oil-contaminated muddy soil in Hanoi, Vietnam

“…Use of petroleum products also cause many harms, especially many types of untreated petroleum are discharged polluting on both soil and water environment (Nandy et al, 2020). Due to its hazardous effects, it is necessary to treat oily appropriately (Khusnuryani et al, 2016).…”

Biofilm formation analysis and molecular identification of copper-resistant bacteria isolated from PT Freeport Indonesia’s tailings

“…The ability of bacteria to form biofilm and grow in it as a microbial consortium is considered to be an important requirement for their survival under harsh environmental conditions. Microbes growing in biofilm community can perform some complex metabolic processes Biofilm-associated bacteria embedded in a matrix of Extracellular Polymeric Substance (EPS) (Annous et al 2009), and the metabolic interaction among bacteria will enhance the degradation process (Cerca et al 2005;Andersson et al 2008;Annous et al 2009;Khusnuryani et al 2014). In this research, we examined biofilm forming ability and identified molecular characteristics of copper-resistant bacteria isolated from PT Freeport Indonesia's tailings.…”