“…( Helotiales incertae sedis ) was able to invade the velamen layer of D. nobile roots, penetrate through passage cells and form fungal pelotons in cortex cells (Hou & Guo 2009). Furthermore, Helotiales members have been repeatedly detected in the rhizosphere and roots of several orchid genera, including Anacamptis (Pecoraro et al 2018), Cattleya (Oliveira et al 2014), Cephalanthera (Julou et al 2005; Abadie et al 2006), Chloraea (Herrera et al 2017), Cymbidium (Shefferson et al 2005; Hong et al 2015), Cypripedium (Oja et al 2015), Dactylorhiza (Schiebold et al 2018), Epipactis (Bidartondo et al 2004; Jacquemyn et al 2016; Xing et al 2020), Goodyera (Shefferson et al 2010), Gymnadenia (Stark et al 2009; Xing et al 2020), Himantoglossum (Pecoraro et al 2013), Malaxis (Schiebold et al 2018), Liparis (Schiebold et al 2018), Neottia (Bidartondo et al 2004; Oja et al 2015; Těšitelová et al 2015), Orchis (Oja et al 2015), Platanthera (Esposito et al 2016), Pleione (Qin et al 2019), Pseudorchis (Kohout et al 2013) and Spiranthes (Tondello et al 2012). However, more morphological and physiological evidence is needed to confirm the mycorrhizal status of Helotiales fungi in orchids.…”