Molecular identification of Theileria equi, Babesia caballi, and Rickettsia in adult ticks from North of Xinjiang, China

Yang, Zhangping; Wen, Xiuxiu; Xiao, Peipei; Fan, Xinli; Li, Min; Chahan, Bayin

doi:10.1002/vms3.613

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…Several genera of ticks, such as Haemaphysalis, Hyalomma, and Dermacentor, can carry and transmit diverse piroplasm pathogens by biting their hosts (Gong et al, 2005 ; Rothschild et al, 2013 ). These ticks are the predominant species in Xinjiang, China (Sheng et al, 2019 ), which has been reported to transmit T. equi and B. caballi (Zhang et al, 2021 ). The sampling sites as a major pastoral area of China were rich with vegetation cover and a diverse geographical environment, which are suitable for the growth and reproduction of ticks (Friedhoff et al., 1990 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Cui,

Cao,

et al. 2024

Veterinary Medicine & Sci

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BackgroundPiroplasmosis is a common and prevalent tick‐borne disease that affects equids.ObjectivesTo determine the infection and molecular characteristics of the piroplasms in donkeys from Xinjiang, northwestern China, we undertook a cross sectional study by collecting representative samples across several counties within the region.MethodsA total of 344 blood samples were collected from adult domestic donkeys from 13 counties in Xinjiang. PCR was conducted to test for T. equi and B. caballi in the blood samples based on the equine merozoite antigen‐1 (Ema‐1) gene and the 48 kDa rhoptry protein (BC48) gene, respectively.ResultsSixteen blood samples tested positive for piroplasms and the overall infection rate was 4.7% (16/344). Seven of the 13 counties were positive for piroplasms. Among the 16 piroplasm‐positive samples, 15 were singly infected with T. equi with an infection rate of 4.4% (15/344), and coinfection with T. equi and B. caballi was detected in one sample (0.3%, 1/344) from Wushi. Four T. equi sequence genotypes were identified and grouped into different branches of the evolutionary trees.ConclusionThese findings suggest that the infection rate of piroplasms is low in domestic donkeys in southern Xinjiang and that T. equi genotypes have a regional distribution.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Cui,

Cao,

et al. 2024

Veterinary Medicine & Sci

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“…[ 18 ]. In China, some molecular and serological investigations targeting B. caballi have been carried out, reporting its presence in multiple provinces including Xinjiang, Jilin, and Gansu [ 19 – 21 ]. Although reports on B. caballi is common, further analysis such as genotyping has been rare.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Zhang,

Gao

et al. 2024

BMC Vet Res

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Babesia spp. and Theileria spp. are tick-borne protozoan parasites with veterinary importance. In China, epidemiological and genetic investigations on many Babesia and Theileria species were still absent in many areas and many tick species. From Aug 2021 to May 2023, 645 ticks were collected from the body surface of domestic animals (camels, goats, sheep, and cattle) using tweezers in seven counties in three provinces including Xinjiang (Qitai, Mulei, Hutubi, and Shihezi counties), Chongqing (Youyang and Yunyang counties), and Qinghai (Huangzhong county). Three tick species were morphologically and molecularly identified (334 Hyalomma asiaticum from Xinjiang, 245 Rhipicephalus microplus from Chongqing, and 66 Haemaphysalis qinghaiensis from Qinghai). A total of three Babesia species and two Theileria species were detected targeting the 18S gene. The COI and cytb sequences were also recovered from Babesia strains for further identification. In R. microplus from Chongqing, Babesia bigemina, the agent of bovine babesiosis, was detected. Notably, in H. asiaticum ticks from Xinjiang, a putative novel genotype of Babesia caballi was identified (0.90%, 3/334), whose COI and cytb genes have as low as 85.82% and 90.64–90.91% nucleotide identities to currently available sequences. It is noteworthy whether the sequence differences of its cytb contribute to the drug resistance of this variant due to the involvement of cytb in the drug resistance of Babesia. In addition, Theileria orientalis and Theileria annulata were detected in R. microplus from Chongqing (12.20%, 31/245) and H. asiaticum from Xinjiang (1.50%, 5/334), respectively. These results suggest that these protozoan parasites may be circulating in domestic animals in these areas. The pathogenicity of the novel genotype of B. caballi also warrants further investigation.

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Molecular identification of Theileria equi, Babesia caballi, and Rickettsia in adult ticks from North of Xinjiang, China

Yang

Wen

Xiao

et al. 2021

Veterinary Medicine & Sci

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Background: Ticks in Xinjiang distribute widely and account for one third of China.Ticks can carry and transmit bacteria, virus, and parasite. However, the research of tickborne pathogens in Xinjiang is rather little.Objective: To understand the situation of hard tick carry Theileria equi, Babesia caballi and Rickettsia spp. of Zhaosu and Altay in Xinjiang. Methods:In this study, 119 tick samples were obtained from horses in Xinjiang, China, Ticks were identified morphologically to determine species and PCR was used to investigate the situation of pathogens by hard ticks.Results: One hundred and seven belong to Dermacentor marginatus, five belong to D. niveus, and seven belong to D. silvarum. Theileria equi and Babesia caballi were detected in one tick and 18 ticks, respectively. However, the carrying rate of Rickettsia spp. was 51.26% (61/119). Among these, the mixed carriage rate of T. equi and Rickettsia spp. was 0.8% (1/119). The mixed carriage rate of B. caballi and Rickettsia spp. was 10.1% (12/119). Conclusion:Our results revealed that hard tick can carry not only haeimoparasite but also many important zoonotic pathogens in Xinjiang, and this situation was worth heeding.

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Molecular identification of Theileria equi, Babesia caballi, and Rickettsia in adult ticks from North of Xinjiang, China

Cited by 4 publications

References 21 publications

Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Molecular detection of piroplasms in domestic donkeys in Xinjiang, China

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Molecular identification of Theileria equi, Babesia caballi, and Rickettsia in adult ticks from North of Xinjiang, China

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