Molecular identification of GHS-R and GPR38 in Suncus murinus

Suzuki, Airi; Ishida, Yuko; Aizawa, Sayaka; Sakata, Ichiro; Tsutsui, Chihiro; Mondal, Anupom; Koike, Kenichi; Sakai, Takafumi

doi:10.1016/j.peptides.2012.04.019

Cited by 36 publications

(33 citation statements)

References 55 publications

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“…The musk shrew has a distinct advantage, similar to mice, as a research model; they are a small animal (40 to 80 g) and can be efficiently used in large numbers. There is also a developing database on the molecular genetics of the GI tract in this species, notably ghrelin and motilin (Ishida et al 2009; Suzuki et al 2012; Sugino et al 2014). Here, I have shown in vivo and in vitro preparations in this species to perform single-unit electrophysiological recordings of GI vagal afferent fibers.…”

Section: Discussionmentioning

confidence: 99%

Measuring the nausea-to-emesis continuum in non-human animals: refocusing on gastrointestinal vagal signaling

Horn

2014

Exp Brain Res

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Nausea and vomiting are ubiquitous as drug side effects and symptoms of disease; however, the systems that determine these responses are arguably designed for protection against food poisoning occurring at the level of the gastrointestinal (GI) tract. This basic biological pathway using GI vagal afferent communication to the brain is not well understood. Part of this lack of insight appears to be related to current experimental approaches, such as the use of experimental drugs, including systemic chemotherapy and brain penetrant agents, which activate parts of the nausea and vomiting system in potentially unnatural ways. Directly related to this issue is our ability to understand the link between nausea and vomiting, which are sometimes argued to be completely separate processes, with nausea as an unmeasurable response in animal models. An argument is made that nausea and emesis are the efferent limbs of a unified sensory input from the GI tract that is likely to be impossible to understand without more specific animal electrophysiological experimentation of vagal afferent signaling. The current paper provides a review on the use of animal models and approaches to define the biological systems for nausea and emesis and presents a potentially testable theory on how these systems work in combination.

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Section: Discussionmentioning

confidence: 99%

Measuring the nausea-to-emesis continuum in non-human animals: refocusing on gastrointestinal vagal signaling

Horn

2014

Exp Brain Res

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“…We previously identified the S. murinus motilin receptor (GPR38) gene and the fact that its expression was high in the stomach (Suzuki et al 2012). In the present study, we analyzed this in more detail and showed that the distribution of GPR38 mRNA expression differs in different parts of the stomach.…”

Section: Motilin(-logm)mentioning

confidence: 99%

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

et al. 2016

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The migrating motor complex (MMC) is responsible for emptying the stomach during the interdigestive period, in preparation for the next meal. It is known that gastric phase III of MMC starts from the proximal stomach and propagates the contraction downwards. We hypothesized that a certain region of the stomach must be more responsive to motilin than others, and that motilin-induced strong gastric contractions propagate from that site. Stomachs of the Suncus or Asian house shrew, a small insectivorous mammal, were dissected and the fundus, proximal corpus, distal corpus, and antrum were examined to study the effect of motilin using an organ bath experiment. Motilin-induced contractions differed in different parts of the stomach. Only the proximal corpus induced gastric contraction even at motilin 10(-10) M, and strong contraction was induced by motilin 10(-9) M in all parts of the stomach. The GPR38 mRNA expression was also higher in the proximal corpus than in the other sections, and the lowest expression was observed in the antrum. GPR38 mRNA expression varied with low expression in the mucosal layer and high expression in the muscle layer. Additionally, motilin-induced contractions in each dissected part of the stomach were inhibited by tetrodotoxin and atropine pretreatment. These results suggest that motilin reactivity is not consistent throughout the stomach, and an area of the proximal corpus including the cardia is the most sensitive to motilin.

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“…Mammalian GHS-R gene is composed of two exons, and two types of GHS-R mRNAs, GHS-R1a and 1b, are generated by alternative transcription process of the gene (Howard et al, 1996). So far, GHS-Rs have been identified in mammals (Howard et al, 1996;Katayama et al, 2000;Kitazawa et al, 2011;Suzuki et al, 2012), avians (Geelissen et al, 2003;Tanaka et al, 2003) and several fish species (Palyha et al, 2000;Chan and Cheng, 2004;Olsson et al, 2008;Kaiya et al, 2009a,b;Small et al, 2009;Eom et al, 2014). In fish, GHS-R1a was first identified by Palyha et al (2000) in pufferfish Takifugu rubripes.…”

Section: Introductionmentioning

confidence: 99%

Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus

Cai

Yuan

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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Molecular identification of GHS-R and GPR38 in Suncus murinus

Cited by 36 publications

References 55 publications

Measuring the nausea-to-emesis continuum in non-human animals: refocusing on gastrointestinal vagal signaling

Measuring the nausea-to-emesis continuum in non-human animals: refocusing on gastrointestinal vagal signaling

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus

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