Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus

Cai, Wenjing; Yuan, Xiaochen; Yuan, Yongchao; Xie, Shouqi; Gong, Yuanyuan; Su, Hang; Qiao, Yingjie

doi:10.1016/j.cbpa.2014.09.009

Cited by 13 publications

(7 citation statements)

References 53 publications

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“…In support of such direct actions of ghrelin on liver cells, our results show for the first time in vertebrates a wide distribution of the ghrelin receptor ghs-r1a in hepatic cells. This is in agreement with the previous identification by PCR of the ghrelin receptor in the liver of some teleosts, including goldfish (Cai et al, 2015;Kaiya et al, 2010).…”

Section: Discussionsupporting

confidence: 93%

Ghrelin induces clock gene expression in the liver of goldfish in vitro via protein kinase C and protein kinase A pathways

Sánchez-Bretaño

Blanco

Alonso-Gómez

et al. 2017

Journal of Experimental Biology

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The liver is the most important link between the circadian system and metabolism. As a food-entrainable oscillator, the hepatic clock needs to be entrained by food-related signals. The objective of the present study was to investigate the possible role of ghrelin (an orexigenic peptide mainly synthesized in the gastrointestinal tract) as an endogenous synchronizer of the liver oscillator in teleosts. To achieve this aim, we first examined the presence of ghrelin receptors in the liver of goldfish. Then, the ghrelin regulation of clock gene expression in the goldfish liver was studied. Finally, the possible involvement of the phospholipase C/protein kinase C (PLC/ PKC) and adenylate cyclase/protein kinase A (AC/PKA) intracellular signalling pathways was investigated. Ghrelin receptor transcripts, ghs-r1a, are present in the majority of goldfish hepatic cells. Ghrelin induced the mRNA expression of the positive (gbmal1a, gclock1a) and negative (gper genes) elements of the main loop of the molecular clock machinery, as well as grev-erbα (auxiliary loop) in cultured liver. These effects were blocked, at least in part, by a ghrelin antagonist. Incubation of liver with a PLC inhibitor (U73122), a PKC activator ( phorbol 12-myristate 13-acetate) and a PKC inhibitor (chelerythrine chloride) demonstrated that the PLC/PKC pathway mediates such ghrelin actions. Experiments with an AC activator (forskolin) and a PKA inhibitor (H89) showed that grev-erbα regulation could be due to activation of PKA. Taken together, the present results show for the first time in vertebrates a direct action of ghrelin on hepatic clock genes and support a role for this hormone as a temporal messenger in the entrainment of liver circadian functions.

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Section: Discussionsupporting

confidence: 93%

Ghrelin induces clock gene expression in the liver of goldfish in vitro via protein kinase C and protein kinase A pathways

Sánchez-Bretaño

Blanco

Alonso-Gómez

et al. 2017

Journal of Experimental Biology

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“…The present study also yielded the rst cDNA sequence of ghsr for bighead carp, which has already been reported for other sh species [27][28][29]. The TM1 to TM7 structures in GHSR were relatively conserved in Cypriniformes [30][31][32]. Additionally, GHSR may function in the cytoplasm because it is a hydrophobic protein without a signal peptide.…”

Section: Discussionsupporting

confidence: 68%

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Wang

Luo

et al. 2023

Preprint

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Backgroud Ghrelin (ghrl) and its receptor gene, ghsr (growth hormone secretagogue receptor), regulate numerous physiological functions, including growth hormone secretion and food intake. In the present study, we investigated the sequences and expression characteristics of the two genes in bighead carp (Hypophthalmichthys nobilis). Methods Cloning and expression characteristics of the ghrl and ghsr gene were got and analyzed by using the RACE technique, qRT-PCR, and in situ hybridization. The sizes of the experimental fish were 1.21 ± 0.20 kg in winter (6–7 months old) and 2.34 ± 0.20 kg in summer (18–19 months old). Results The ghrl and ghsr genes, encoded 106 and 346 amino acids, respectively, and were 1208 bp and 2239 bp in length. Early blastocysts had the highest levels of ghrl and ghsr expression throughout early development (P < 0.01). The gut exhibited the highest levels of expression of ghrl, whereas significantly higher levels of ghsr (P < 0.01) were detected in the brain. Significantly higher levels of expression of ghrl and ghsr were detected in the intestine and muscle in summer, when compared with the levels in winter (P < 0.05). Based on in situ hybridization, intestine ghrl expression signals were more pronounced than intestinal ghsr expression signals, whereas ghsr expression signals were higher than those of ghrl in brain tissues. The co-expression patterns of related genes in the growth hormone synthesis, secretion and action pathway (ko04935), and cAMP signaling pathway (ko04024) also indicated that ghrl and ghsr are involved in the feeding regulation of bighead fish. Conclusion Our findings suggest that ghrl and ghsr potentially play roles in bighead carp feeding and associated energy metabolism activities, in addition to growth performance.

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“…Ghrelin receptors in fish pituitary have been poorly investigated, but low basal expression levels have been found in tilapia ( 56 ), goldfish ( 59 , 60 ) or yellow catfish ( 61 ). In the case of grass carp, 14, 21, and 28 days of fasting resulted in increased pituitary gene expression of ghsr1a that correlated with increased plasma Gh and preproghrelin pituitary gene expression ( 62 ). Moreover, these authors found that Ghrelin administration provoked an increase in pituitary ghsr1a expression.…”

Section: Discussionmentioning

confidence: 99%

Ghrelin and Its Receptors in Gilthead Sea Bream: Nutritional Regulation

et al. 2018

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Ghrelin is involved in the regulation of growth in vertebrates through controlling different functions, such as feed intake, metabolism, intestinal activity or growth hormone (Gh) secretion. The aim of this work was to identify the sequences of preproghrelin and Ghrelin receptors (ghsrs), and to study their responses to different nutritional conditions in gilthead sea bream (Sparus aurata) juveniles. The structure and phylogeny of S. aurata preproghrelin was analyzed, and a tissue screening was performed. The effects of 21 days of fasting and 2, 5, 24 h, and 7 days of refeeding on plasma levels of Ghrelin, Gh and Igf-1, and the gene expression of preproghrelin, ghsrs and members of the Gh/Igf-1 system were determined in key tissues. preproghrelin and the receptors are well conserved, being expressed mainly in stomach, and in the pituitary and brain, respectively. Twenty-one days of fasting resulted in a decrease in growth while Ghrelin plasma levels were elevated to decrease at 5 h post-prandial when pituitary ghsrs expression was minimum. Gh in plasma increased during fasting and slowly felt upon refeeding, while plasma Igf-1 showed an inverse profile. Pituitary gh expression augmented during fasting reaching maximum levels at 1 day post-feeding while liver igf-1 expression and that of its splice variants decreased to lowest levels. Liver Gh receptors expression was down-regulated during fasting and recovered after refeeding. This study demonstrates the important role of Ghrelin during fasting, its acute down-regulation in the post-prandial stage and its interaction with pituitary Ghsrs and Gh/Igf-1 axis.

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Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus

Cited by 13 publications

References 53 publications

Ghrelin induces clock gene expression in the liver of goldfish in vitro via protein kinase C and protein kinase A pathways

Ghrelin induces clock gene expression in the liver of goldfish in vitro via protein kinase C and protein kinase A pathways

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Ghrelin and Its Receptors in Gilthead Sea Bream: Nutritional Regulation

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