Molecular identification of fungi associated with mulberry root rot disease in Eastern and North Eastern India

Pappachan, Anil; Rahul, K.; Irene, L.; Sivaprasad, V.

doi:10.22271/09746315.2020.v16.i1.1291

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…Also from her study, she could not establish a clear correlation between genetic diversity and geographical distribution. Many researchers in other crops, identified different species of Lasiodiplodia, however, from our studies, we could only identify L. theobromae causing BRR of mulberry in India (Pappachan et al, 2020;Radhakrishnan et al, 1995;Sowmya et al, 2018;Sukumar and Padma 1999) and this is the first detailed phylogenetic analysis of L. theobromae causing BRR of mulberry in India using two genes.…”

Section: Discussionmentioning

confidence: 78%

“…4), the bootstrap values were low (53%) and the sequence homology of these four isolates was 98% as compared to the other isolates which had a high similarity of 100% with the type strain with high bootstrap values (83%). Likewise, Pappachan et al (2020), isolated L. theobromae isolate from the infected roots of mulberry collected from Mizoram, Northeast India has 99.03% homologous to L. theobromae Bl16 (MK813947) with low bootstrap value. Sowmya et al (2018) based on cultural, morphological, pathogenicity, SSR and RAPD markers revealed significant variations among the L. theobromae isolates obtained from the infected gardens of Karnataka, Andhra Pradesh and Tamil Nadu.…”

Section: Discussionmentioning

confidence: 99%

“…Lasiodiplodia species are harmful fungal pathogens and have been associated with nearly 500 plant hosts causing various degrees of crop losses. It causes root rot disease in mulberry that affects all parts of the plant and it spreads rapidly affecting a large number of plants in a short period leading to the abandonment of mulberry gardens (Pappachan et al, 2020). The fungus prefers 25-30°C for good growth and survives on all types of substrates.…”

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confidence: 99%

“…There are limited data on BRR of mulberry in India, and the previous characterization of L. theobromae was based on morphology. Sowmya et al (2018) used random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSRs) to study the genetic variability, among the ten isolates of L. theobromae causing BRR of mulberry, similarly, Pappachan et al (2020) characterized only one isolate of L. theobromae. However, these studies could not reveal the molecular phylogeny of isolates and did not attempt to identify resistant sources to L. theobromae.…”

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confidence: 99%

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Characterization and Pathogenicity of Lasiodiplodia theobromae Causing Black Root Rot and Identification of Novel Sources of Resistance in Mulberry Collections

et al. 2022

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Black root rot (BRR) caused by Lasiodiplodia theobromae is an alarming disease of mulberry that causes tremendous economic losses to sericulture farmers in India and China. Successful control of this disease can be attained by screening germplasm and identifying resistant sources. Seventy four diseased root samples were collected from farmer’s fields belonging to four major mulberry growing states of South India. Based on morpho-cultural and scanning electron microscopy studies, 57 fungal isolates were characterized and identified as L. theobromae. Phylogenetic analysis of concatenated internal transcribed spacer and β-tubulin sequences revealed variation of the representative 20 isolates of L. theobromae. Following the root dip method of inoculation, pathogenicity studies on susceptible mulberry genotypes (Victory-1 and Thailand male) recognized the virulent isolate MRR-142. Accordingly, MRR-142 isolate was used to evaluate resistance on a set of 45 diverse mulberry accessions. In the repeated experiments, the mulberry accession ME-0168 which is an Indonesian origin belonging to Morus latifolia was found to be highly resistant consistently against BRR. Eight accessions (G2, ME-0006, ME-0011, ME-0093, MI-0006, MI-0291, MI-0489, and MI-0501) were found to be resistant. These promising resistant resources may be exploited in mulberry breeding for developing BRR resistant varieties and to develop mapping populations which successively helps in the identification of molecular markers associated with BRR.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization and Pathogenicity of Lasiodiplodia theobromae Causing Black Root Rot and Identification of Novel Sources of Resistance in Mulberry Collections

et al. 2022

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“…Pathogens were isolated from the infected fruits collected during the survey by tissue segment method. The diseased portion of the fruits were surface sterilized with 1% NaOCl for 1-2 min and rinsed thrice with repeated changes of sterile distilled water (Pappachan et al, 2020). The surface sterilized tissues were inoculated onto sterile PDA medium supplemented with streptomycin sulphate (0.03 g/l) and incubated for 5 days at 28±2C.…”

Section: Isolation Of Fruit Rot Pathogensmentioning

confidence: 99%

Understanding the Etiology of Chilli Fruit Rot Disease in Tamil Nadu

Renuka¹,

Prabakar²,

Pugalendhi³

et al. 2022

ASD

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Background: Fruit rot is an age old destructive disease of chilli caused by complex fungal pathogens resulting in severe yield losses both at pre and post-harvest stages. Hence, this study aims to investigate the occurrence of fruit rot disease in Tamil Nadu and to identify the fungal pathogens associated with fruit rot symptoms based on morpho-molecular characters. Methods: Roving survey was conducted in various districts of Tamil Nadu to determine the prevalence of fruit rot disease and to ascertain its causative agents. The pathogens were identified based on the conidial morphology, cultural characteristics and further confirmed by Polymerase chain reaction (PCR) using ITS 1 and ITS 4 primers. Result: Maximum disease severity was documented in Dharmapuri followed by Namakkal and lowest in Nagapattinam district. Ten isolates representing two genera viz., Colletotrichum and Fusarium were recovered from fruit rot infected chilli fruits and validated by pathogenicity test. Based on the morpho-cultural characters, 2 isolates were identified as Fusarium sp., with oval/ellipsoidal microconidia and straight/curved macroconidia, 6 isolates as Colletotrichum scovillei with fusiform conidia and 2 isolates as Colletotrichum truncatum with falcate conidia. The virulent isolates were further confirmed as C. scovillei based on PCR amplification of ITS region of genomic DNA.

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