Molecular Identification of Fish Larvae from East Plawangan of Segara Anakan, Cilacap, Central Java, Indonesia

Nuryanto, Agus; Pramono, Hendro; Sastranegara, Moh. Husein

doi:10.15294/biosaintifika.v9i1.9191

Cited by 15 publications

(34 citation statements)

References 19 publications

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“…Similar result was also resulted when the samples were subjected to barcode of life data identification system (BOLD system) where the strains showed the sequence similarity of 98.79%-100% to the sequences of O. guramy available in BOLD system. Nuryanto et al (2017) also found a low intraspecific genetic divergence on fish larvae collected in East Plawangan, Segara Anakan, Cilacap. 0.394 ** 0,551 *** -0,009 ns -Note: * = 0.05 ≥ p ≥ 0.01, ** = 0.01 > p ≥ 0.001, *** = p < 0.001; ns = not significant 14.053 0.455 F ST : 0.508 *** Note: Va and F ST : p-value = 0.000 ± 0.000; * = 0.05 ≥ p ≥ 0.01, ** = 0.01 > p ≥ 0.001, *** = p < 0.001; NS = not significant The result of sequence divergence analysis supported the morphological data, which placed the giant gourami populations from Java and Sumatra into different strains, except for Tambago and Oranye strains.…”

Section: Genetic Divergences Among Strainsmentioning

confidence: 81%

Molecular characterization of four giant gourami strains from Java and Sumatra

et al. 2018

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Nuryanto A, Amalia G, Khairani D, Pramono H, Bhagawati D. 2018. Molecular characterization of four giant gourami strains from Java and Sumatra. Biodiversitas 19: 528-534. Giant gourami (Osprhonemus goramy) are widely distibuted across Indonesia, such Java, Sumatra, and Kalimatan which lead to the emerge of various gourami strains due to morphological differences. However, no scientific data about the relationship between morphological and genetic differences among strains. This research aimed to obtain information on molecular characteristics of four giant gourami strains from Java and Sumatra based on partial sequences of cytochrome c oxidase 1 gene. This information is vital to strengthen their taxonomic status. Caudal fin clips were sampled from each strain. Nucleotide sequencing was performed using bigdye terminator tehcnique. Pairwise Fst comparison was carried out using arlequin software, whereas sequences of divergence analysis was performed in DnaSP software. Homology of the sequences were checked with previous published data available in Boldsystem data base. Homology test resulted in 98.79 to 100% similarity to the previous published sequences. This means that all strains belonged to single species, i.e. Osphronemus goramy. This placement was supported by low-level of genetic divergences among strains. Although they have low-level genetic divergences, this value is suitable to separate each strain clearly as indicated by pairwise Fst comparison analysis and AMOVA, which showed differences among strains. However, phylogenetic tree shows that all stains formed a monopyletic group with boostratp value of 100. Phylogenetic analysis supports the placement of all strains into a single species that is O. guramy. Those morphological differences are also reflected in their genetic character, except for Tambago and Oranye strains.

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Section: Genetic Divergences Among Strainsmentioning

confidence: 81%

Molecular characterization of four giant gourami strains from Java and Sumatra

et al. 2018

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“…The power of this gene as a species barcode marker because it is changeable enough (Nuryanto et al, 2007;Nuryanto and Kochzius, 2009) due to its high mutation rate which is higher compared to other mitochondrial genome (Hebert et al, 2003). Clear separation of different species and above species category was also reported in fish larvae (Nuryanto et al, 2017), or even among strain within species (Nuryanto et al, 2018).…”

Section: Homology Test and Genetic Species Conceptmentioning

confidence: 94%

“…These observed high values of inter-species genetic divergences are common phenomena. Several studies also reported high genetic divergences among species in various animal groups, such as in fish (Peg et al, 2006;Pereira et al, 2013;Nuryanto et al, 2017;Nuryanto et al, 2018); and Crustacea (Barber et al, 2002).…”

Section: Homology Test and Genetic Species Conceptmentioning

confidence: 97%

“…The cytochrome c oxidase 1 (CO1) is widely used as barcode marker in such studies (Mabragaña et al, 2011;Radulovici et al, 2010) and it is believed as highly precise marker for species identification (deWaard et al, 2011). It has been reported that CO1 barcode could properly identify fish (von der Heyden et al, 2014;Hubert et al, 2012;Muricy, 2011;), even from fillet samples (Wong and Hanner, 2008); and fish larvae with limited morphological features (Pegg et al, 2006;Nuryanto et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago

et al. 2018

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Spermonde Archipelago in South Sulawesi is among groupers sources in Indonesia for International Marine Live Trade. It was not much known which species are mostly exploited in those areas during the study because only fin clip samples could be obtained. Here we performed DNA forensic analysis to groupers fin clips collected from Spermonde Archipelago using barcode marker to know what species were exploited and which ones was the most exploited species. A total of 110 fin clip samples were obtained during the field trips. Sequencing of the cytochrome c oxidase 1 gene was successful for only 36 samples. Species determination was based on sequences similarity to conspecific sequence available in barcoding of life database (BOLD). BOLD similarity test placed the 36 fin clip samples into four different species, namely Cromileptes altivelis, Epinephelus ongus, Plectropomus leopardus, and Plectropomus maculatus. The K-2P taxonomic tree showed clear separation among species which was supported by high K2P genetic distances among species. Most of fin clips were identified as Plectropomus leopardus indicates that this species was the most exploited in Spermonde Archipelago. This proved that molecular identification not only can be used to biodiversity study in certain area but also in forensic analysis of a threatened wildlife. This study has important contribution for conservation effort of those groupers species, especially P. leopradus and provides new approach for taxonomist and conservation managers to identify samples, especially when only incomplete specimens are available.

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“…However, there are many factors affecting the growth of gourami, such as body size and parasite infection. Nuryanto et al (2017) reported that there were genetic variations in the body size of gourami. reported that the prevalence of Trichodina sp.…”

Section: Introductionmentioning

confidence: 99%

Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java

2018

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Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body's morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 '-AAC CTG GTT GAT CCT GCC ATG-3') and Reverse primer (5 '-TGA TCC TTC TGC AGG TTC ACC TAC-3') which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java

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Molecular Identification of Fish Larvae from East Plawangan of Segara Anakan, Cilacap, Central Java, Indonesia

Cited by 15 publications

References 19 publications

Molecular characterization of four giant gourami strains from Java and Sumatra

Molecular characterization of four giant gourami strains from Java and Sumatra

Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago

Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java

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