Molecular identification of Candida species from urinary infections in Honduras

Ortiz, Bryan; Pérez-Alemán, Erika; Galo, Carmen; Fontecha, Gustavo

doi:10.1016/j.riam.2017.07.003

Cited by 18 publications

(32 citation statements)

References 26 publications

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“…In urine, however, the most frequent species were C. albicans complex, C. tropicalis and C. glabrata complex. A previous study in search of the primary cause of genitourinary infection in a hospital in Honduras analyzed 73 yeasts with results consistent with the current study [21].…”

Section: Comparison Of Microscan ® Hardychrom ® and Pcr-rflpsupporting

confidence: 89%

“…analyzed Candida strains isolated from six types of clinical samples, and also reported an important predominance of C. albicans [29]. Other studies have analyzed a single type of clinical sample, such as blood [30][31][32][33], urine [21,24], vulvovaginal secretion [34], bronchoalveolar fluid [35], and gastroesophageal tract [36], however in all of them the predominant species was C. albicans.…”

Section: Comparison Of Microscan ® Hardychrom ® and Pcr-rflpmentioning

confidence: 99%

“…Molecular identification of Candida species was performed using a widely used method based on PCR-RFLP[21,23,24]. The amplification reaction was directed to the ribosomal region comprising the ITS1 and ITS2 spacers and the 5.8S gene.…”

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confidence: 99%

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Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

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Ortiz

Galindo

et al. 2019

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Candida species are one of the most important causes of human infections, especially in hospitals and among immunocompromised patients. The correct and rapid etiological identification of yeast infections is important to provide adequate therapy, reduce mortality and control outbreaks. In this study, Candida species were identified in patients with suspected fungal infection, and phenotypic and genotypic identification methods were compared. A total of 167 axenic fungal cultures and 46 clinical samples were analyzed by HardyCHROM®, MicroScan®, and PCR-RFLP. The species of the C. albicans complex were the most frequent, followed by C. tropicalis and C. glabrata. Less common but clinically relevant species of Candida were also isolated. The comparison between the three methods was concordant, especially for the most common Candida species. Fungal DNA amplification was successful in all clinical samples.

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Section: Comparison Of Microscan ® Hardychrom ® and Pcr-rflpsupporting

confidence: 89%

Section: Comparison Of Microscan ® Hardychrom ® and Pcr-rflpmentioning

confidence: 99%

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Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

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Ortiz

Galindo

et al. 2019

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“…DNA from the axenic cultures was extracted following a previously described protocol using a BeadBeater ® system (Bio Spec products Inc., Bartlesville, OK, USA) with glass beads, and phenolchloroform [33]. The four Candida complexes were identified by PCR-RFLP, amplifying the ITS ribosomal region and performing DNA digestion using the enzyme MspI [33,34]. According to this method, the species within the complexes C. albicans, C. parapsilosis, and C. haemulonii cannot be differentiated from each other, since they produce similar restriction patterns.…”

Section: Pcr-rflp Of the Its Region And Mspimentioning

confidence: 99%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

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Candida spp. are the most common cause of fungal infections worldwide. The taxonomy of Candida is controversial and has undergone recent changes due to novel genetically related species. Therefore, some complexes of cryptic species have been proposed. In clinical settings, the correct identification of Candida species is relevant since some species are associated with high resistance to antifungal drugs and increased virulence. This study aimed to identify the species of four Candida complexes (C. albicans, C. glabrata, C. parapsilosis, and C. haemulonii) by molecular methods. This is the first report of six cryptic Candida species in Honduras: C. dubliniensis, C. africana, C. duobushaemulonii, C. orthopsilosis, and C. metapsilosis, and it is also the first report of the allele hwp1-2 of C. albicans sensu stricto. It was not possible to demonstrate the existence of C. auris among the isolates of the C. haemulonii complex. We also propose a simple method based on PCR-RFLP for the discrimination of the multi-resistant pathogen C. auris within the C. haemulonii complex.

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“…As maiores taxas de isolamento de Candida podem ser encontradas em indivíduos com idade superior a 60 anos. Em relação ao gênero, é reportada maior ocorrência de candidúria em mulheres do que em homens (OZER;DURMAZ;YULA, 2016;ORTIZ et al, 2018). Para contribuir no diagnóstico microbiológico, podem ser citados os derivados de 2-fenilbenzoxazol para serem utilizados em ensaios fluorogênicos dada sua alta capacidade de emissão de fluorescência (HOLLER et al, 2002).…”

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Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

Souza

Corbellini

2019

RJP

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Neste estudo foram sintetizados três derivados de 2-fenilbenzoxazol (2FBO) funcionalizados com a função azo: (2’-hidroxi-5’-fenilazofenil)benzoxazol (HFAZBO), 2-(2’-hidroxi-5’-(4’’-carboxi)fenilazofenil)benzoxazol (HFAZBO-CO2H) e 2-(2’-hidroxi-5’-(4’’-nitro)fenilazofenil)benzoxazol (HFAZBO-NO2).Os compostos foram utilizados como substratos fluorogênicos para agentes de infecção urinária, representantes de Candida spp., para explorar suas características metabólicas. A caracterização estrutural dos compostos foi realizada por meio de Espectroscopia de Absorção Molecular no Infravermelho com transformada de Fourier (FT-IR) e por determinação da faixa de fusão. Os ensaios fluorogênicos foram realizados em espécies de Candida spp.: Candida albicans ATCC 10231, Candida famata RL38, Candida glabrata 06130L, Candida glabrata RL43, Candida glabrata 0030L, Candida guilliermondii TH07, Candida krusei ATCC 6258, Candida krusei 0037L, Candida parapsilosis (RL33), Candida parapsilosis (RL38), Candida stellatoidea (9875), Candida stellatoidea (3613), Candida tropicalis ATCC 750. Não foi observada alteração sob luz uv nas leveduras testadas. Do mesmo modo, o extrato obtido a partir das colônias não apresentou fluorescência sob luz uv. Dessa forma, faz-se necessário desenvolver novos estudos que avaliem a função metabólica de Candida spp. a partir da biotransformação levando a liberação de moléculas fluorescentes.

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Molecular identification of Candida species from urinary infections in Honduras

Cited by 18 publications

References 26 publications

Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Síntese e avaliação de azoderivado de 2-fenilbenzoxazol como substrato fluorogênico na diferenciação de Candida spp.

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