Molecular identification and typing of Mycobacterium massiliense isolated from postsurgical infections in Brazil

Monego, Fernanda; Duarte, Rafael Silva; Nakatani, Sueli M.; Araújo, Wildo Navegantes de; Riediger, Irina Nastassja; Brockelt, Sonia Regina; Souza, Verena Maria Mendes de; Cataldo, Jamyra Iglesias; Dias, Rubens Clayton da Silva; Biondo, Alexander Welker

doi:10.1590/s1413-86702011000500004

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…However, at the moment, no study has identified how and why the same clone was introduced and able to spread rapidly in different locations of the country. 4,[7][8][9] The genetic analysis of 11 of our samples had 100% identity with the BRA100 clone, confirming that this outbreak was part of the countrywide outbreak of M. abscessus subsp. bolletii, associated with video-assisted surgery.…”

Section: Discussionsupporting

confidence: 59%

“…Further molecular analysis identified a predominant clone, called BRA100, as the causative agent of infections in different states since 2004. 4,[7][8][9] One of the peculiarities of this species is the resistance to 2% glutaraldehyde, even after 10 hours of exposure. 2 investigations, investigators hypothesized that flaws related to cleaning, disinfection, and sterilization of surgical equipment such as immersion of the surgical instruments into sterilizing solution (eg, glutaraldehyde) could be involved with the occurrence of RGNTM infections.…”

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confidence: 99%

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Risk Factors forMycobacterium abscessussubsp.bolletiiInfection After Laparoscopic Surgery During an Outbreak in Brazil

Villar

Freitas

Ramos

et al. 2015

Infect. Control Hosp. Epidemiol.

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OBJECTIVE To identify risk factors related to Mycobacterium abscessus subsp. bolletii infection during an outbreak, associated with laparoscopic surgery and to propose recommendations for preventing new cases. DESIGN A retrospective cohort study. SETTING A private hospital in Manaus, Brazil. PATIENTS A cohort of 222 patients who underwent laparoscopic surgery between July 2009 and August 2010 by a single surgical team. METHODS We collected information about the patients and the surgical procedure using a standard form. We included sex, age, and variables with P≤0.2 in the bivariate analysis in a logistic regression model. Additionally, we reviewed the procedures for reprocessing the laparoscopic surgery equipment, and the strains obtained with culture were identified by molecular methods. RESULTS We recorded 60 (27%) cases of infection. After multivariate analysis, the duration of surgery beyond 1 hour (odds ratio [OR] 2.4; 95% confidence interval [CI] 1.2-4.5), not to have been the first operated patient on a given day (OR, 2.7; 95% CI, 1.4-5.2), and the use of permanent trocar (OR, 2.2; 95% CI, 1.1-4.2) were associated with infection. We observed that the surgical team attempted to sterilize the equipment in glutaraldehyde solution when sanitary authorities had already prohibited it. Eleven strains presented 100% DNA identity with a single strain, known as BRA100 clone. CONCLUSIONS Because contaminated material can act as vehicle for infection, ensuring adequate sterilization processing of video-assisted surgery equipment was crucial to stopping this single clonal outbreak of nonturbeculous mycobacteria in Brazil.

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Section: Discussionsupporting

confidence: 59%

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confidence: 99%

Risk Factors forMycobacterium abscessussubsp.bolletiiInfection After Laparoscopic Surgery During an Outbreak in Brazil

Villar

Freitas

Ramos

et al. 2015

Infect. Control Hosp. Epidemiol.

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“…In regard to ATP levels, post-cleaning were comparable with pre-contamination results. After contamination, ATP levels reached a median of 8434 (range: 1385-727668) RLUs, whereas after cleaning levels decreased to 35 (range: 11-220) RLUs, comparable with the pre-contamination levels of 28 (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) RLUs. After cleaning, only one catheter displayed more than 100 RLUs (220 RLUs,…”

Section: Adenosine Triphosphate Testsupporting

confidence: 54%

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Neves

Silva

Ventura

et al. 2016

Gastrointestinal Endoscopy

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“…4 Rapid growing mycobacteria (RGM) can form biofilms drastically affecting immunocompromised hosts, and the infection progression are challenging to control due to multi-drug resistance profiles against different antibiotics, 5 such as clarithromycin, imipenem, 6 rifampicin, isoniazid, ethambutol, pyrazinamide, 7 cefoxitin, and doxycycline. 8 Mycobacterium fortuitum is mainly present in skin, soft tissue and catheter associated infections, 9 while Mycobacterium abscessus noticeably accounts for pulmonary infections 10 and Mycobacterium massiliense for post-surgical ones. 11 Considering the reduced introduction of novel antibiotics in the market and the increasing resistance to the commonly used in mycobacterial infections, the urge for new antimycobacterial agents is a reality.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Evaluation of New Eugenol-Derived 1,2,3-Triazoles as Antimycobacterial Agents

Santos

Coelho

Elias

et al. 2019

J. Braz. Chem. Soc.

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Eugenol has diverse biological properties including antimycobacterial activity, and the triazole ring is an important heterocycle in antimycobacterial compounds. Therefore, this research aimed to synthesize novel eugenol-derived 1,2,3-triazole as antimycobacterial agents with interesting cytotoxic profile and pharmacological assets. Sixteen compounds were obtained and characterized by nuclear magnetic resonance (NMR), infrared (IR), and high-resolution mass spectrometry (HRMS). Among them, the best growth inhibition properties from a microdilution assay were observed for three derivatives: a benzylic ether (minimum inhibitory concentration (MIC) = 48.89 µM) against Mycobacterium abscessus (ATCC 19977), an O-galactosyde (MIC = 31.76 µM) against Mycobacterium massiliense (ATCC 48898) and a sulfonate (MIC = 88.64 µM) against Mycobacterium fortuitum (ATCC 6841). They can form biofilms, and the infection progression is challenging to control due to multi-drug resistance profiles against diverse antibiotics. In conclusion, the above-mentioned compounds represent starting points in the search of bioactive molecules against mycobacteria with low cytotoxicity and better pharmacological profiles.

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Molecular identification and typing of Mycobacterium massiliense isolated from postsurgical infections in Brazil

Cited by 3 publications

References 22 publications

Risk Factors forMycobacterium abscessussubsp.bolletiiInfection After Laparoscopic Surgery During an Outbreak in Brazil

Risk Factors forMycobacterium abscessussubsp.bolletiiInfection After Laparoscopic Surgery During an Outbreak in Brazil

Effectiveness of current disinfection procedures against biofilm on contaminated GI endoscopes

Synthesis and Biological Evaluation of New Eugenol-Derived 1,2,3-Triazoles as Antimycobacterial Agents

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