Molecular identification and phylogenetic analyses of multiple nucleopolyhedrovirus isolated from Lymantria obfuscata (Lepidoptera: Lymantriidae) in India

Gani, Mudasir; Gupta, Rakesh Kumar; Zargar, Sajad Majeed; Kour, Gurpreet; Monobrullah,; Kandasamy, Thamilarasi; Mohanasundaram, A.

doi:10.1007/s13355-017-0490-3

Cited by 7 publications

(1 citation statement)

References 37 publications

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“…On the other hand, sequencing of specific baculovirus genes have been widely used to establish phylogenetic relationships between different species and strains, using highly conserved genes (Kaur et al 2014;Gani et al 2017). Sequences of lef-8, lef-9, and polh/granulin genes have been recommended to be used as a minimum information to detect phylogenetic relationships between strains (Jehle et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Virulence and genetic characterization of six baculovirus strains isolated from different populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Zanella-Saenz

Herniou²,

Ibarra³

et al. 2022

Arch Microbiol

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Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPV-Arg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An2, SfNPV-Sin and SfGV-RV.To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9, and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGV-VG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

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Section: Discussionmentioning

confidence: 99%