Molecular identification and characterization of the dog motilin receptor

Ohshiro, Hiroyuki; Nonaka, Miki; Ichikawa, Keita

doi:10.1016/j.regpep.2007.08.012

Cited by 32 publications

(22 citation statements)

References 28 publications

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“…From in vivo and in vitro experiments using S. murinus, we demonstrated that motilin-induced gastric contractions are mediated through the myenteric plexus (Mondal et al 2011;Sakahara et al 2010). Similar results have been reported in other species (De Smet et al 2009;Kitazawa et al 1995;Mizumoto et al 1993;Ohshiro et al 2008;Van Assche et al 1997).…”

Section: Introductionsupporting

confidence: 86%

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

et al. 2016

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The migrating motor complex (MMC) is responsible for emptying the stomach during the interdigestive period, in preparation for the next meal. It is known that gastric phase III of MMC starts from the proximal stomach and propagates the contraction downwards. We hypothesized that a certain region of the stomach must be more responsive to motilin than others, and that motilin-induced strong gastric contractions propagate from that site. Stomachs of the Suncus or Asian house shrew, a small insectivorous mammal, were dissected and the fundus, proximal corpus, distal corpus, and antrum were examined to study the effect of motilin using an organ bath experiment. Motilin-induced contractions differed in different parts of the stomach. Only the proximal corpus induced gastric contraction even at motilin 10(-10) M, and strong contraction was induced by motilin 10(-9) M in all parts of the stomach. The GPR38 mRNA expression was also higher in the proximal corpus than in the other sections, and the lowest expression was observed in the antrum. GPR38 mRNA expression varied with low expression in the mucosal layer and high expression in the muscle layer. Additionally, motilin-induced contractions in each dissected part of the stomach were inhibited by tetrodotoxin and atropine pretreatment. These results suggest that motilin reactivity is not consistent throughout the stomach, and an area of the proximal corpus including the cardia is the most sensitive to motilin.

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Section: Introductionsupporting

confidence: 86%

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

et al. 2016

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“…Accordingly, alternative laboratory animals are needed to translate in vitro studies on motilin into pharmacodynamic and physiological functions in vivo . For this purpose, motilin receptors have been characterized in the rabbit 17 and dog 13 …”

Section: Discussionmentioning

confidence: 99%

“…This was necessary because of marked species differences in motilin receptor pharmacology. Thus, a functional motilin system is absent in rodents (mice, rats, guinea‐pigs), 11,12 and although expressed in dogs, agonists at the dog receptor may be less potent than at the human motilin receptor 13 …”

Section: Introductionmentioning

confidence: 99%

GSK962040: a small molecule motilin receptor agonist which increases gastrointestinal motility in conscious dogs

Leming

Broad

Cozens

et al. 2011

Neurogastroenterology Motil

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“…Motilin has been identified in many mammals (Bond et al, 1988; Banfield et al, 1992; Xu et al, 2001; Strausberg et al, 2002; Xu e al., 2003; Ohshiro et al, 2008) and chickens (DeClercq et al, 1996). Motilin binding sites are present in the gastrointestinal tract and central nervous system, along with a G-protein-coupled receptor, GPR38, which has been identified as MLNR (Feighner et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Association between <italic>Motilin Receptor</italic> Gene Haplotypes and Growth Traits in Japanese Hinai-dori Crossbred Chickens

Takahashi

Rikimaru

Komatsu

et al. 2014

Asian Australas. J. Anim. Sci

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We previously identified quantitative trait loci (QTL) for body weight and average daily gain in a common region between ADL0198 (chr 1: 171.7 Mb) and ABR0287 (chr 1: 173.4 Mb) on chicken chromosome 1 in an F2 resource population produced by crossing low- and high-growth lines of the Hinai-dori breed. Motilin receptor (MLNR) is a candidate gene affecting growth traits in the region. In this study, we genotyped polymorphisms of the MLNR gene and investigated its association with growth traits in a Hinai-dori F2 intercross population. All the exons of the MLNR gene in the parental population were subjected to PCR amplification, nucleotide sequenced and haplotypes identified. To distinguish resultant diplotype individuals in the F2 population, a mismatch amplification mutation assay was performed. Three haplotypes (Haplotypes 1–3) were accordingly identified. Six genotypes produced by the combination of three haplotypes (Haplotype 1, 2, and 3) were examined in order to identify associations between MLNR haplotypes and growth traits. The data showed that Haplotype 1 was superior to Haplotype 2 and 3 in body weight at 10 and 14 weeks of age, average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age in female in F2 females. It was concluded that MLNR is a useful marker of growth traits and could be used to develop strategies for improving growth traits in the Hinai-dori breed.

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Molecular identification and characterization of the dog motilin receptor

Cited by 32 publications

References 28 publications

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

GSK962040: a small molecule motilin receptor agonist which increases gastrointestinal motility in conscious dogs

Association between <italic>Motilin Receptor</italic> Gene Haplotypes and Growth Traits in Japanese Hinai-dori Crossbred Chickens

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Molecular identification and characterization of the dog motilin receptor

Cited by 32 publications

References 28 publications

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

The proximal gastric corpus is the most responsive site of motilin-induced contractions in the stomach of the Asian house shrew

GSK962040: a small molecule motilin receptor agonist which increases gastrointestinal motility in conscious dogs

Association between &lt;italic&gt;Motilin Receptor&lt;/italic&gt; Gene Haplotypes and Growth Traits in Japanese Hinai-dori Crossbred Chickens

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Association between <italic>Motilin Receptor</italic> Gene Haplotypes and Growth Traits in Japanese Hinai-dori Crossbred Chickens