Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.)

Abstract

Plant growth promoting rhizobacteria (PGPR) are a group of useful bacteria that colonize the plant roots and significantly enhances the plant growth promotion. Keeping this in mind, an investigation was performed for the screening of potent PGPR strains for enhancing seed germination and vigour index of Hordeum vulgare (commonly called barley). Rhizobacterial strains were isolated and screened for various plant growth promoting traits, their effect on seed germination and vigour index of barley plant through pot trial, and resistant ability under various temperature and pH range. Based upon 16S rRNA sequencing data, Pseudomonas punonensis LMT03 (R1), Pseudomonas plecoglossicida R4, Pseudomonas aeruginosa DSM50071 (R2) and Alcaligenes faecalis (DBHU5) isolates were selected and showed positive result for IAA production, Phosphate solubilization, ammonia production, catalase activity, siderophore production and MR-VP test. Barley plants treated with P. punonensis and P. plecoglossicida both showed 94.44% of highest seed germination %, while P. aeruginosa and A. faecalis showed 83.11% and 77.33% respectively in comparison to the control plant shows which 49.99% seed germination only. These respective isolates also showed 2.57, 2.37, 2.0 and 1.69 fold of increase in vigour index as compare to the control plants. The above increase in fold in vigour index and seed germination is much higher as compared to earlier reports. Collectively, the data of current study underpin that addition of these PGPRs to barley rhizosphere appears a promising strategy to enhance root and shoot biomass of this important agriculture crop. To the best of our knowledge this is the first report demonstrating the effect of P. punonensis and P. plecoglossicda on barley crop.

The Screening of Plant Growth-promoting Rhizobacteria based on the Ability to Promote the Growth and Nodulation of Soybean (Glycine max L.) Seedlings

“…5,6 They can support plant growth directly through Nitrogen fixing from the atmosphere, mineral dissolution, such as phosphate, siderophores production, and growth hormone syntheses, such as Indole Acetic Acid (IAA), gibberellin acid, cytokines, and ethylene, 7 besides that the PGPR application is useful in accelerating seed germination and seedling growth. 8 Rhizobium bacteria is one of the bacteria that can live in the soil or inside a root nodule formed from the symbiotic mutualism with the host plant. 9 One Rhizobium species is incapable of spinning in every legume plant.…”