Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt

Aaty, Heba E. Abdel; Abdel-Hameed, Dina M; Alam-Eldin, Yosra Hussein; el-Shennawy, S F; Aminou, Heba; Makled, Sameh; Darweesh, Samar K

doi:10.1016/j.actatropica.2011.10.014

Cited by 46 publications

(32 citation statements)

References 21 publications

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“…All sheep cysts were the cysticercus of Taenia hydatigena which is known as cysticercus tenuicollis as reported by Azab et al (2004) and Aaty et al (2012). However, Abd El Baki et al (2009Tawfeek et al (2009);Taha (2012) reported the presence of hydatid cysts in sheep.…”

Section: Fig 1 Map Showing Distribution Of Ce Patients In Egyptian mentioning

confidence: 67%

“…Few molecular studies have identified the current status of E. granulosus genotypes in Egypt. Moreover, Egyptian reports concerning the predominant E. granulosus genotype in human and animal isolates are controversial (Azab et al 2004;Abd El baki et al 2009;Tawfeek et al 2009;Aaty et al 2012). Therefore, the present study aimed to ascertain the genotype(s) of E. granulosus responsible for human hydatidosis in different Egyptian governorates (regions).…”

Section: Introductionmentioning

confidence: 91%

“…Comparison of the Egyptian G7 genotype with the Slovakian G7 of Dinkel et al (2004) showed 100% identity. The obtained G6 genotype nucleotide sequence is identical to the Egyptian G6 deposited by Aaty et al (2012).…”

Section: G5/6/7 Pcr Assay and Dna Sequencing For Human And Animal Isomentioning

confidence: 98%

“…To date, 7 genotypes have been identified in Middle East and North Africa: G1, G2, G3, G4, G5, G6 and G7 (Sadjjadi 2006;Omer et al 2010;Ahmed et al 2013;Adwan et al 2013;Cardona and Carmena 2013;Alvarez Rojas et al 2014). Only G1, G4 and G6 have been characterized in Egypt (Abd El Baki et al 2009;Aaty et al 2012;Aboelhadid et al 2013). Few molecular studies have identified the current status of E. granulosus genotypes in Egypt.…”

Section: Introductionmentioning

confidence: 94%

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Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Alam-Eldin

Aaty

Ahmed

2015

Acta Parasitologica

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Few molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis.The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.

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Section: Fig 1 Map Showing Distribution Of Ce Patients In Egyptian mentioning

confidence: 67%

Section: Introductionmentioning

confidence: 91%

Section: G5/6/7 Pcr Assay and Dna Sequencing For Human And Animal Isomentioning

confidence: 98%

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Alam-Eldin

Aaty

Ahmed

2015

Acta Parasitologica

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“…Therefore, to determine transmission cycle, the study of G3 genotype reservoirs as intermediate hosts in this region is essential. In a study in Egypt (Abdel Aaty et al 2012), 42 sheep hydatid cyst samples were genotyped that all samples were reported as G6 genotype. Also in Pakistan (Latif et al 2010), one- …”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran

et al. 2014

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Echinococcus granulosus as an etiologic agent of hydatid cyst is one of the most important zoonotic helminthes in the world that causing enormous economic and health losses. The aim of this study was to evaluate genotype of E. granulosus isolated from sheep using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and sequencing method in East Azerbaijan province, northwest of Iran. Nineteen sheep hydatid cyst samples were collected. Genomic DNA was extracted from protoscoleces using commercial DNA extraction kit. Mitochondrial cox1 region was amplified by polymerase chain reaction (PCR) and all isolates were sequenced. Afterward, sequences were analyzed for determination of genotypes by related software. G1 (94.73 %) and G3 (5.27 %) genotypes were identified from the isolates which out of 19 hydatid cysts, 17 samples were G1B, 1 sample G1D and the other one had G3 genotype. Results of this study indicate that common sheep strain (G1); especially G1B is the dominant subtype of E. granulosus in East Azerbaijan province.

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Genetic variations among Echinococcus granulosus isolates in Egypt using RAPD-PCR

Taha

2012

Parasitol Res

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Cystic echinococcosis (CE), caused by hydatid cysts, is a widespread and hazardous disease in humans and animals worldwide. The aim of the current study was to investigate the genetic variations among Echinococcus granulosus cyst strains isolated from sheep, camel, pig, and donkey using RAPD-PCR analysis. Seven primers of arbitrary sequences were used in the PCR reactions. The screened primers gave total patterns ranging from 27 to 39 reproducible bands for each isolate. Each population isolate gave its specific pattern. Although distinct polymorphic patterns were obtained among the four isolates, there were several shared bands among them in each primer used. A comparison of the different RAPD-PCR patterns showed that primers P1, P3, and OPH 04 yielded band patterns that revealed a high degree of divergence among the four isolates of E. granulosus that allowed easy distinction between them. The remaining primers (P2, P4, P5, and OPH14) amplified DNA fragments that were common to two or more isolates but diversified in the other two or three isolates. The study revealed that the most closely related isolates were of donkey and camel where the similarity coefficent between them ranging from 53 % to 78 %, followed by isolates of pig and sheep (sc = 40 % to 68 %), while the similarity coefficent between isolates of camel and sheep was 33-45 %, between camel and pig was 36 to 57 %, between donkey and pig was 37 to 52 %, and between donkey and sheep was 35 to 54 % which means that they more or distant from each other. In conclusion, hydatid cysts isolated from camel may have the genotypic characters of donkey strain.

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Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt

Cited by 46 publications

References 21 publications

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran

Genetic variations among Echinococcus granulosus isolates in Egypt using RAPD-PCR

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