Molecular – genetic variance of RH blood group system within human population of Bosnia and Herzegovina

Lasić, Lejla; Lojo-Kadric, Naida; Silajdžić, Elma; Pojskić, Lejla; Hadziselimovic, Rifat; Pojskić, Naris

doi:10.17305/bjbms.2013.2403

Cited by 5 publications

(10 citation statements)

References 12 publications

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“…The five most important antigens from this blood group system are the D, C, c E and e since they are highly immunogenic. The C, c, E and e antigens are less immunogenic than the D antigen but can still cause immunogenic reactions [1]. The RH locus is bigenic consisting of the RHD and RHCE genes located on the short arm p34-36 of chromosome 1 with an order of 5'-RHD-3'-3'-RHCE-5'.…”

Section: Introductionmentioning

confidence: 99%

“…Serological typing is faster and is the first choice for routine testing but has its own limitations, such as testing is only done on red blood cells (RBC's), thus only the phenotype is known and reagents are sometimes costly [7,8]. In addition haemagglutination assays are not ideal for recently transfused patients, patients with autoantibodies and it cannot distinguish between different RHD variants [1,9]. In these cases serology techniques are difficult or impossible to carry out or else give inconclusive results, thus in Malta, the sample is referred abroad for genotyping.…”

Section: Introductionmentioning

confidence: 99%

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Determining the Genotype of the RH Blood Group System in Maltese Blood Donors

Powney,

Borg,

Debono

2023

IBRR

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Aims: The Rh blood group system is a complex protein based system having the D, C, c, E and e as the most immunogenic antigens. This blood group system has a vital role in transfusion medicine thus by DNA typing, important medical issues in transfusion practice can be resolved when serological techniques fail. The aim of the study was to determine the frequency of the RH genotype in the Maltese blood donors using molecular techniques. Methodology: 797 blood donor samples were enrolled in this study. An allele-specific polymerase chain reaction (AS-PCR) method was used to determine the presence of RHD, RHCE*E and RHCE*e, while multiplex PCR was used to test for RHCE*C/c. Results: Out of 797 samples studied, the most common allele was RHCE*e with a percentage of 98. The most common genotype in RhD positive samples was DCcee and in RhD negative samples, the most common was dccee. The most frequent haplotype was DCe. Conclusion: Like in previous studies, this research also concludes that the distribution of the RH genotype varies in different geographical areas. Further studies working with larger sample size and better techniques would share better light on the molecular function of these loci.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determining the Genotype of the RH Blood Group System in Maltese Blood Donors

Powney,

Borg,

Debono

2023

IBRR

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“…Blood erythrocyte group antigens are the genetically strictly deterministic feature. In spite of resistant specific characteristics, the relevant hereditary factors within the ranges of species at the level of populations and individuals are characterized with rather high polymorphism [10] [11] [12] [13]. The Rhesus system consists of 49 defined antigens.…”

Section: Introductionmentioning

confidence: 99%

The Features of Antigen Prevalence of Rhesus System in Donor Population

Nagervadze

Akvlediani

Tsintsadze

et al. 2021

International Journal of Biology and Biomedical Engineering

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Research materials and methods. 852 voluntary Georgian blood donors have been typed on red blood cells group antigens. The research materials have taken from the diagnostic laboratory of Health Centre of Batumi (Georgia republic). The immunoserological methods with monoclonal anti –AB, -B, -A, A1, -A2 (H), -C, -c, - D, -E, -e (Bio-Rad, cypress diagnostics) antibodies was used for typing blood. The ID cards, such as ABO/D + Reverse Grouping (Bio-Rad) were also used for typing of erythrocyte antigens. Result. Prevalence of Rh system antigens in the studied group is looks like so: e antigens – 94,6%, c antigens -85%, C-68,03, E antigens - 38,07%. The majority (84%) of the studied donors are Rh-positive (n=719), 133 (16%) donors are Rh-negative. C antigen most common is present in the combination with D antigen. 65, 8 % case donors had CD+ combination (n=561). E antigen in most cases is presented with a combination of D antigen. 36, 9% of the studied donors (n=306) had ED+ combination. A miserable number of studied donors had CD - (2,23%; n=19) and ED - (1,17%; n=9) combinations. We have studied the Rh phenotypes prevalence in blood donors. According to RHD, RHC, and RHE gene loci, there are 18 theoretically possible phenotypical groups. Among them half (nine) are Rh-positive and the rest of them are Rh-negative. The Rh-positive phenotypes are: CDE; CDEe; CDe; CcDE; CcDEe; CcDe; ccDE; cDEe and cDe. Rh-negative phenotypes are CdE; CdEe; Cde; CcdE; CcdEe; Ccde; cdE; cdEe; cde. We allocated 17 Rh phenotypes among studied donors. Only one phenotype CdE, which belongs to Rh negative group, was not present in studied donors. Other 17 phenotypes showed different frequencies. Some of them were only in a single case, for example, cdEe, cdE, CdEe phenotypes had only one donor. The majority of the phenotype in he studied donors (27,8±1,53%) was CcDe (n=237). CcDEe -19,3±1,35% (n=165); 125 donors have CDe phenotype (14,6±1,2); The frequency of cde was 13,1±1,5%, which means that 112 studied donors belonged to this phenotype group; 87 studied donors had cDEe phenotype characteristics (10,2%); The frequency of cDe was 4,9% (n=42); 19 donors had CDEe phenotype. Other phenotypes (CDE, Cde, CcdEe, Ccde) frequency was very low. Conclusion. Our studied donors are characterized by rather high polymorphism. The Georgian donor’s population is heterogenic, especially high heterogeneity are shown in Rh positive phenotypes. The obtained data is vital importance for the preparation of whole blood or certain blood components for the purpose of their rational usage in blood transfusion.

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“…Bioanthropological research in Bosnia and Herzegovina began during the Austro-Hungarian Empire in the late 19th century, and the history of such research is presented in Lasic et al 2016. A number of bioanthropological and genetic studies have been performed in Bosnia and Herzegovina to assess the heterogeneity of local populations as well as the general B&H population (Berberovic 1969;Dogan et al 2016;Hadziselimovic 1981;Kapur-Pojskic et al 2014;Kovacevic et al 2013Kovacevic et al , 2014Lasic et al 2013Lasic et al , 2016Lasic et al , 2020Marjanovic et al 2005Marjanovic et al , 2005aMarjanovic et al , 2006Marjanovic et al , 2008Pojskic et al 2013). However, most of these works are based on molecular genetic markers, and a smaller part on phenotypic markers.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic association of seven observed traits of hand complex

Kalajdžić

Pojskić

Čorbo

et al. 2021

J. bioanthropol. (Online)

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This paper provides an overview of the seven possible hand-related traits, with an idea to estimate the statistical phenotypic association between them. The traits observed in this study were: midphalangeal hair, Hitchhiker’s thumb, extensibility of proximal joint of thumb, digital index, nail shape, crooked fifth finger and hand clasping. The mentioned characteristics were observed in 7431 unrelated subjects, with approximately equal gender distribution. For each case, three groups were formed: total, male and female. The subjects were evenly distributed throughout Bosnia and Herzegovina’s various geographical regions, and they properly represent the country’s national and ethnic composition. The Chi-squared test was used to determine the statistical significance of the association between these traits, while Fisher’s exact test was utilized as an extra test to analyze the association between each pair of observed features. Also, Chi-squared test was applied for observing differences in the frequencies of the phenotypic characteristics of the hand between the genders. Following traits were shown to have a statistically significant association: midphalangeal hair-Hitchhiker’s thumb, midphalangeal hair – digital index, midphalangeal hair – nail shape, midphalangeal hair – crooked fifth finger, Hitchhiker’s thumb- extensibility of proximal joint of thumb, digital index – nail shape, digital index -crooked fifth finger, midphalangeal hair – hand clasping, crooked fifth finger – hand clasping. Analysis of differences in the frequencies of the observed phenotypic traits of the hand according to the genders showed statistical significance for D, Dht, Ref and Lf.

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Molecular – genetic variance of RH blood group system within human population of Bosnia and Herzegovina

Cited by 5 publications

References 12 publications

Determining the Genotype of the RH Blood Group System in Maltese Blood Donors

Determining the Genotype of the RH Blood Group System in Maltese Blood Donors

The Features of Antigen Prevalence of Rhesus System in Donor Population

Phenotypic association of seven observed traits of hand complex

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