Molecular-Genetic Mechanisms of Plasmodium Falciparum Virulence and Tropical Malaria Pathogenesis

Усков, А. Н.; Соловьев, А. И.; Kravtsov, Viacheslav; Гудков, Р. В.; Коломоец, Е. В.; Левковский, А. Е.

doi:10.22625/2072-6732-2018-10-3-23-29

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…Подобные полиорганные поражения, связанные с продолжающейся гибелью эритроцитов, известны, однако требуют дополнительного изучения с использованием современных молекулярно-генетических методов исследования плазмодиев [10,11]. Данное направление является предметом собственных текущих исследований [12,13].…”

Section: данные объективного обследования общее состояние тяжелое оunclassified

Complexity of the therapy of P. falciparum - malaria in the Russian Federation

Kovalenko

Николаевич²,

Мусатов

et al. 2019

Terapevticheskii arkhiv

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The article presents a clinical case of malaria in a 29-year - old man without immunodeficiency, complicated by the development of infectious - toxic shock, anemia, kidney damage. The brief literature review discusses the complexity of etiotropic therapy, the problem of pathogen resistance to antimalarial drugs, pathogenetic mechanisms that contribute to the formation of shock, anemia and kidney damage in tropical malaria.

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Section: данные объективного обследования общее состояние тяжелое оunclassified

Complexity of the therapy of P. falciparum - malaria in the Russian Federation

Kovalenko

Николаевич²,

Мусатов

et al. 2019

Terapevticheskii arkhiv

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Development of a technique for analyzing the lengths of restriction fragments to assess the mefloquine resistance of plasmodium falciparum based on the identification of single-nucleotide polymorphisms (A958146T, A961013G, G961625T) of the PFMDR1 gene

Arukov

Соловьев

Krutikova

et al. 2023

Bulletin of the Russian Military Medical Academy

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ABSTRACT. A method for identifying single nucleotide polymorphisms of the PfMDR1 gene (A958146T, A961013G, G961625T) based on the analysis of the lengths of restriction fragments using polymerase chain reaction technology with specific primers is presented. To identify the polymorphism A958146T (amino acid substitution N86Y), it was proposed to amplify a fragment of parasitic deoxyribonucleic acid with a length of 417 bp, including the 86th codon of the PfMDR1 gene, followed by treatment of the amplicon with ApoI endonuclease, the restriction site of which includes the sequence of the analyzed mutation. With the Plasmodium falciparum genotype unchanged, the isolated deoxyribonucleic acid site is divided into two fragments of size 239 and 179 bp. In the case of an altered genotype containing a single nucleotide missense mutation A T, the original fragment 417 bp will be preserved. When designing the system to detect the amino acid substitution N1042D caused by the A961013G mutation, a pair of specific primers were selected that limit the 404 bp-long section of parasitic deoxyribonucleic acid. For restriction analysis, the most optimal was the use of AseI endonuclease, which in the case of an unchanged Plasmodium falciparum genotype divides the initial amplicon into 4 fragments (132, 116, 99 and 25 bp), and in the presence of A G mutation into 3 (248, 99, 25 bp). It was found that a fragment of the PfMDR1 gene, including the G961625T mutation leading to the amino acid substitution D1246Y, contains 1 site corresponding to the restriction site of endonuclease BglII. Therefore, in the case of the wild genotype of Plasmodium falciparum, the initial fragment of deoxyribonucleic acid is cut into 2 short sections (300 and 269 bp). With the D1246 mutation, the replacement of the nucleotide G T leads to the disappearance of the restriction site, so only one source fragment (509 bp) will be recorded on the electrophoregram. Based on the analysis of the data obtained, criteria for evaluating the drug resistance of Plasmodium falciparum have been developed. The decrease in sensitivity to mefloquine and its derivatives of pathogens of tropical malaria can be evidenced by positive results obtained using the developed methods for detecting haplotypes A T (N86Y (band 417 bp)), A G (N1042D (bands 248, 99 and 25 bp)), G T (D1246Y (509 bp)). The developed methods can be used to analyze the spread of drug-resistant tropical malaria.

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Clinical case of imported tropical malaria in a teenager

Бондаренко

2023

Det. infekc.

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В статье представлен случай завозной тропической малярии у девочки 15 лет, которая за 5 дней до начала болезни вернулась из Объединенной Республики Танзания. Заболевание началось с повышения температуры тела до 38-40°С, катаральных явлений. С 3-го дня болезни присоединились боли в животе, рвота, жидкий стул. Несмотря на ранее обращение за медицинской помощью (2-й день болезни) и своевременно собранный эпидемиологический анамнез, диагноз тропической малярии был установлен на 6-й день болезни, когда развились осложнения заболевания. Позднее начало адекватной терапии привело к летальному исходу от полиорганной недостаточности на 7-й день болезни.

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Molecular-Genetic Mechanisms of Plasmodium Falciparum Virulence and Tropical Malaria Pathogenesis

Cited by 4 publications

References 22 publications

Complexity of the therapy of P. falciparum - malaria in the Russian Federation

Complexity of the therapy of P. falciparum - malaria in the Russian Federation

Development of a technique for analyzing the lengths of restriction fragments to assess the mefloquine resistance of plasmodium falciparum based on the identification of single-nucleotide polymorphisms (A958146T, A961013G, G961625T) of the PFMDR1 gene

Clinical case of imported tropical malaria in a teenager

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