Molecular genetic identification of arbuscular mycorrhizal fungi

Yurkov, Andrey P.; Павлович, Юрков Андрей; Kryukov, Alexey A.; Анатольевич, Крюков Алексей; Gorbunova, Anastasia; Олеговна, Горбунова Анастасия; Kojemyakov, A. P.; Петрович, Кожемяков Андрей; Степанова, Галина; Васильевна, Степанова Галина; Мачс, Э. М.; Модрисович, Мачс Эдуард; Rodionov, Alexandr V.; Викентьевич, Родионов Александр; Шишова, М. Ф.; Федоровна, Шишова Мария

doi:10.17816/ecogen16211-23

Cited by 12 publications

(11 citation statements)

References 76 publications

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“…For example, some works mentioned that different sets of taxa can be detected in the analysis of fungal ITS1 and ITS2 [7][8]. Results of some studies, including those we conducted earlier [1,5,9], showed the detected AMF sequences very often have significant differences from sequences presented in the databases. For example, the NCBI database contains <50% of AMF species [4][5].…”

Section: Introductionmentioning

confidence: 95%

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Identification of arbuscular mycorrhizal fungi in soils of the North Caucasus based on Illumina MiSeq data for ITS1 and ITS2 regions

et al. 2020

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The objective of our research was to analyze the efficiency of identification of arbuscular mycorrhizal fungi (AMF) for 2 regions: ITS1 and ITS2 regions of AMF DNA isolated from the soils of the North Caucasus (Karachay-Cherkessia). For the first time the necessity of different AMF species identification using both ITS regions was revealed, but not one region. The research demonstrated: 1) the set of taxa is different using ITS1- and ITS2-based identification; 2) analysis of the ITS1 region reveals a greater number of operational taxonomic units; 3) ITS2 allows identification of AMF at the species level more often. Sample preparation for Illumina MiSeq analysis was optimized. Obligatory stages in the sample preparation were the purification of DNA in the agarose gel in Silica after isolation, as well as separate amplification of ITS1 and ITS2 followed by combining and joint sequencing for each sample. The results showed the highest AMF biodiversity for the 176Te sample from the ecosystem of the subalpine meadow of the southeastern slope of Malaya Hatipara mountain (43°25′48.0″N 41°42′31.0″E; 2401 m above sea level), in which 8 species of AMF were identified (Archaeospora spainiae, Claroideoglomus claroideum, Diversispora versiformis, Entrophpora infrequens, Funneliformis mosseae, Glomus indicum, Paraglomus laccatum, Rhizophagus irregularis).

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Section: Introductionmentioning

confidence: 95%

“…Morphological identification often does not allow a clear separation of closely related species [1]. At the same time, views of various authors on the systematics of Glomeromycotina radically differ [2].…”

Section: Introductionmentioning

confidence: 99%

Identification of arbuscular mycorrhizal fungi in soils of the North Caucasus based on Illumina MiSeq data for ITS1 and ITS2 regions

et al. 2020

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“…Certain successes have been achieved in studying the role of endomycorrhiza and its most common form -arbuscular mycorrhiza (AM), which is typical for most modern phylogenetic groups of plants and is introduced in all biomes of the globe. It is formed by fungi belonging to the Glomeromycotina subdivision of the Mucoromycota division (Yurkov et al, 2018). However, the use of arbuscular mycorrhizal fungi (AMF) in crop production is limited, which is a consequence of their obligate symbiotrophy (Ijdo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

Bukharina

Islamova

2021

Agraria

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The effect of inoculation with endotrophic micromycete Cylindrocarpon magnusianum on the formation of adaptive physiological and biochemical reactions in test tomato plants under the action of heavy metal salts has been studied. The experimental design included plant inoculation with a fungus culture (control population) and these fungus populations were preliminarily grown on a pentose-dextrose agar medium with the addition of heavy metal salts in different concentrations. The inoculated plants were grown on substrates without the addition of heavy metal salts (control) and with the addition of zinc, copper, lead, and chromium salts in different concentrations similar to additions in agar when growing fungus populations. In experimental plants, we determined the content of photosynthetic pigments and ascorbic acid in the leaves (spectrophotometry), nitrates in the leaves (ionometric analysis), the aerial part and root system biomass by the gravimetric method, and the degree of fungal infection development of plants via the light microscopy method of macerated and colored fragments of the plant root system. No stimulatory effect, increasing the resistance of plants to the action of heavy metal salts when plants were inoculated with a control population of the C. magnusianum fungus, was revealed. A positive effect was revealed during the inoculation of plants with fungal populations, previously adapted to the action of salts of chemical elements, especially chromium and lead salts, and during further plants' cultivation on substrates with the introduction of these non-biogenic chemical elements hazardous to the life of plants. The effect is associated with the stability of the pigment system, the redistribution of plant biomass, and the synthesis of the antioxidant-ascorbic acid in the leaves. Intensive development of fungal infection in plant roots was also noted. The study results indicate the most effective partnership between the C. magnusianum fungus and the plant root system under stressful conditions for plant life.

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“…Different sets of fungal taxa can be discovered by using ITS1 and ITS2 as barcodes [8][9]. Many detected in experimental studies sequences of AM fungi are absent in genetic databases [2,6,10]. The greatest database GenBank NCBI contains less than 50% of found species of AM fungi [5][6].…”

Section: Introductionmentioning

confidence: 99%

Illumina-based identification of arbuscular mycorrhizal fungi from Karachay-Cherkessia soils for development of bio-fertilizers

Yurkov

Kryukov

Mikhaylova³

et al. 2021

E3S Web Conf.

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The aim of the study was to investigate the species diversity of AM fungi in different parts of the North Caucasus, biodiversity hotspot, the center of the world’s biological diversity. Samples were taken from 5 locations (stationary trial plots, STPs) in different ecosystems and at various altitudes. Identification was performed using sequencing for ITS1 and ITS2 regions, amplified with universal primers, Illumina MiSeq was employed. 19 genera of AM fungi were found on all STPs. The work did not reveal a correlation between the altitude and the species composition of AM fungi. At the same time, it should be assumed that a correlation could be found between the biodiversity of AM fungi and the type of ecosystem, which should be done in the future. The study shows it is necessary to use an analysis for both ITS regions, since the data obtained for each ITS region differ and complement each other. Analysis for the ITS2 region revealed 1.3 times more virtual taxa than for the ITS1, while the number of OTUs identified per species was similar for both regions. The highest biodiversity of AM fungi was found in STP #3 (with meadow flora). Only 4 species (Rhizophagus irregularis, R. intraradices, Paraglomus laccatum, and Claroideoglomus claroideum) were found on all five analyzed STPs. We found unexpectedly that with such a high biodiversity among the identified fungi, no different species were found in the Paraglomus genus, all the sequences of Paraglomus belonged to Paraglomus laccatum, whereas at least 9 species are distinguished in the genus by morphology. Further research will allow us to identify new strains of AM fungi, the efficiency of which may be higher than already studied ones. In the future this will make it possible to create more effective microbial biofertilizers for agriculture.

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Molecular genetic identification of arbuscular mycorrhizal fungi

Cited by 12 publications

References 76 publications

Identification of arbuscular mycorrhizal fungi in soils of the North Caucasus based on Illumina MiSeq data for ITS1 and ITS2 regions

Identification of arbuscular mycorrhizal fungi in soils of the North Caucasus based on Illumina MiSeq data for ITS1 and ITS2 regions

The effect of inoculation of the root system with Cylindrocarpon magnusianum on plant performance exposed to heavy metal salts

Illumina-based identification of arbuscular mycorrhizal fungi from Karachay-Cherkessia soils for development of bio-fertilizers

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