Molecular genetic characteristics of mcr-9-harbouring Salmonella enterica serotype Typhimurium isolated from raw milk

Wang, Xiaoming; Ling, Zhuoren; Sun, Naiyan; Liu, Yongshi; Huang, Jinhu; Wang, Liping

doi:10.1016/j.ijantimicag.2021.106332

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…Although the majority of previously reported mcr-9 -carrying isolates, including eight Citrobacter spp. isolates in this study, were not resistant to colistin ( 18 ), it has been proved that the expression of mcr-9 could be inducible by subinhibitory concentrations of colistin in the presence of qseB and qseC genes, the MIC levels were therefore increased ( 19 ). This suggests that the clinical use of colistin may induce resistance to colistin in mcr - 9 -positive isolates and accelerate the dissemination of mcr - 9 among potential pathogens.…”

Section: Discussionmentioning

confidence: 82%

Epidemiology and Molecular Characteristics of mcr-9 in Citrobacter spp. from Healthy Individuals and Patients in China

Wang

Yang

et al. 2022

Microbiol Spectr

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The emergence of mcr homologues posed a serious threat to the therapeutic efficiency of polymyxin antibiotics. Citrobacter freundii is generally regarded as an opportunistic pathogen associated with a variety of nosocomial infections. In this study, we investigated the prevalence of mcr-9 in Citrobacter spp. isolates from healthy individuals and infected patients and highlighted the importance of the rational use of antibiotics.

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Section: Discussionmentioning

confidence: 82%

Epidemiology and Molecular Characteristics of mcr-9 in Citrobacter spp. from Healthy Individuals and Patients in China

Wang

Yang

et al. 2022

Microbiol Spectr

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“…The two plasmids that we characterized in our study, pS15 and pS639, also belonged to the IncHI2 type, both of them having IncHI2 and IncHI2A replicons, which indicates that they are hybrid plasmids. This type of plasmid is conjugative which can result in extensive spread of the mcr-9 gene in recipient hosts ( Ai et al, 2021 ; Elbediwi et al, 2021a ; Khodor et al, 2021 ; Wang et al, 2021 ). Testing whether the plasmids we discovered can be transmitted to other strains, we found that pS15 was indeed conjugative to both, Salmonella and E. coli .…”

Section: Discussionmentioning

confidence: 99%

“…Compared to the low level colistin resistance mediated by mcr-1 , most of the mcr-9 -carrying strains do not present resistance to colistin ( Luo et al, 2017 ; Wang et al, 2021 ). We also observed this to be the case with the strains S15 and S639, which we described in this study.…”

Section: Discussionmentioning

confidence: 99%

Molecular Genetic Characteristics of Plasmid-Borne mcr-9 in Salmonella enterica Serotype Typhimurium and Thompson in Zhejiang, China

et al. 2022

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Salmonella enterica is a zoonotic food-borne pathogen threatening public health around the world. As is the case with many other pathogens, the spread of mobilized colistin resistance (mcr) alleles is of grave concern. In this study, totally 689 clinical Salmonella isolates were collected from a local hospital in Hangzhou, Zhejiang Province, China between 2009 and 2018. Resistance genes were screen by PCR. Two mcr-9-positive Salmonella strains S15 and S639 were identified which belong to serotype Typhimurium and Thompson, respectively. We observed that both mcr-9 genes were located on conjugative IncHI2 plasmids which encoded numerous resistance genes, likely facilitating the dissemination of mcr-9 by co-resistance mechanisms. The mcr-9 cassettes encoded on the two plasmids were not identical: downstream of the mcr-9 genes, we found IS1 on one plasmid (pS15), while the other had a WbuC-IS26 (pS639). Despite the presence of mcr-9 cassettes, the strains were not rendered colistin resistant. Yet, it is of epidemiological importance to implement surveillance to be able to observe and possibly control the spread of mcr-9 due to its potential to mediate resistance to the last-resort antibiotic colistin.

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“…[70,77] It is usually founded in the Enterobacteriaceae family, which includes the majority of Salmonella species as well as a few E. coli and other members. [21,[78][79][80] It has been globally distributed in 21 countries across six continents including Asia (n = 27; 19.6%), Europe (n = 72; 52.2%), Africa (n = 1; 0.7%), South-America (n = 1; 0.7%), North-America (n = 27; 19.6%), and Oceania (n = 10; 7.2%). [78] IncHI2-type plasmid is significantly associated with mcr-9, according to comparative genomics.…”

Section: Global Dissemination Of Plasmid Mediated Colistin Resistance...mentioning

confidence: 99%

“…It is reported that the presence of mcr‐9 is not associated with resistance to colistin or polymyxin B but it is possible that any changes in mcr‐9 gene sequence could alter the effects of mcr ‐9 on colistin susceptibility in future. [ 79–81 ] Further researchers reported that mcr‐9 was inducible in the presence of colistin when it was positioned upstream of the TCS qseBC. For example, colistin sensitivity was found in 30 gram‐negative bacteria that had mcr‐9 but lacked the qseBC‐TCS.…”

Section: Global Dissemination Of Plasmid Mediated Colistin Resistance...mentioning

confidence: 99%

Colistin Resistance in Aquaculture Environment: An Emerging Challenge

Dwivedi

Awasthi

2023

Macromolecular Symposia

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The emergence of colistin resistance is one of the most serious future issues since it is a last‐resort antibiotic used to treat infections caused by multidrug‐resistant (MDR) gram‐negative bacteria, particularly those resistant to carbapenems. The aquatic environment acts as a reservoir for the emergence and spread of colistin resistant pathogens. The emergence and transmission of a plasmid mediated mcr gene is one of the most important mechanism of colistin resistance in gram negative bacteria. Several mcr variants have been identified in zoonotic pathogens of aquatic origin that may easily transmit this gene to human pathogens. Currently, very few studies have been conducted to test colistin susceptibility in aquaculture associated bacteria. This may be attributed to a limited technical resource for screening colistin‐resistant microorganisms. As a result, to reduce the emergence and spread of colistin resistance in human and animal health, as well as in the environment, the One Health strategy and laboratory methodologies must be expanded.

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Molecular genetic characteristics of mcr-9-harbouring Salmonella enterica serotype Typhimurium isolated from raw milk

Cited by 12 publications

References 18 publications

Epidemiology and Molecular Characteristics of mcr-9 in Citrobacter spp. from Healthy Individuals and Patients in China

Epidemiology and Molecular Characteristics of mcr-9 in Citrobacter spp. from Healthy Individuals and Patients in China

Molecular Genetic Characteristics of Plasmid-Borne mcr-9 in Salmonella enterica Serotype Typhimurium and Thompson in Zhejiang, China

Colistin Resistance in Aquaculture Environment: An Emerging Challenge

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