Molecular Gender Determination of Ancient Human from Malay Peninsular

Abstract

Problem statement: DNA samples from fourteen modern human bloods (seven males and seven females) and two ancient skeletal samples excavated from Kalumpang Island and Dungun, Peninsular Malaysia were subjected for molecular genders determination using specific primers of human AMELX and AMELY. Approach: A standard multiple PCR mixture with forward primer and either a human X-specific reverse primer for AMELX or a human Y-specific reverse primer for AMELY amplifications were used to assess the presence of these genes in male and female samples. Results: PCR amplification of a modern male sample yielded 329 and 235 bp bands, whereas a modern female sample only 329 bp band. The Kalumpang Island sample produced two positive bands of AMELY and AMELX. After reamplification of the Dungun sample, only an AMELY band was visible. All amplified bands were cloned into TA plasmid and sequenced. BLAST analysis showed that the 329 bp band is AMELX, while the 235 bp product is AMELY. Conclusion/Recommendations: The skeletal remains of both Kalumpang Island and Dungun samples from west and east of Peninsular Malaysia respectively, are males.

Diş Örneklerinden ve İnsan İskelet Parçalarından DNA"nın PCR ile Çoğaltılmasında Etkili Bir Yöntem

“…Under certain circumstances, such as rapid desiccation, low temperatures or high salt concentrations, nucleases can themselves become destroyed or inactivated before all nucleic acids are reduced to mononucleotides. If this is the case, slower but still relentless processes start affecting the DNA 17 . Furthermore, deamination, depurination and other hydrolytic processes will lead to destabilization and breaks in DNA molecules.…”

Penentuan Umur Manusia menggunakan Rongga Pulpa Gigi Pramolar Pertama melalui Pendekatan Cameriere

“…Gigi menjadi pilihan utama dalam menganggar umur individu kerana gigi didapati lebih tahan lama terhadap proses degradasi serta tidak mudah musnah akibat faktor persekitaran (Cunha et al 2009). Selain itu, pendekatan genetik molekul juga boleh dilakukan untuk penentuan jantina melibatkan kromosom seks (Shahrul Hisham et al 2009) dan filogenetik yang melibatkan DNA mitokondria (Sahidan et al 2014). Penentuan kaum etnik Kadazan Dusun dari kepulauan Borneo boleh ditentukan melalui pendekatan Cephalometric dan Malocclusion yang melibatkan bahagian tengkorak manusia (Rohaya et al 2013).…”