Molecular Epidemiology of the Integron-Located VEB-1 Extended-Spectrum β-Lactamase in Nosocomial Enterobacterial Isolates in Bangkok, Thailand

Girlich, Delphine; Poirel, Laurent; Leelaporn, Amornrut; Karim, A.; Tribuddharat, Chanwitt; Fennewald, Michael; Nordmann, Patrice

doi:10.1128/jcm.39.1.175-182.2001

Cited by 126 publications

(130 citation statements)

References 46 publications

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“…The M. smegmatis mc 2 155 arr gene product exhibits more than 50% identity to ADP ribosyltransferases from Streptomyces coelicolor, Pseudomonas aeruginosa (26), Klebsiella pneumoniae (1), E. coli, and other enterobacteria (6,7,15). In the enterobacteria, rifampin ribosylation, mediated by arr-2 gene cassettes, is a clinically important mechanism of drug resistance.…”

Section: Discussionmentioning

confidence: 99%

A Rifampin-Hypersensitive Mutant Reveals Differences between Strains of Mycobacterium smegmatis and Presence of a Novel Transposon, IS 1623

Alexander

Jones

Liu

2003

Antimicrob Agents Chemother

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Rifampin is a front-line antibiotic for the treatment of tuberculosis. Infections caused by rifampin-and multidrug-resistant Mycobacterium tuberculosis strains are difficult to treat and contribute to a poor clinical outcome. Rifampin resistance most often results from mutations in rpoB. However, some drug-resistant strains have rpoB alleles that encode the phenotype for susceptibility. Similarly, non-M. tuberculosis mycobacteria exhibit higher levels of baseline resistance to rifampin, despite the presence of rpoB alleles that encode the phenotype for susceptibility. To identify other genes involved in rifampin resistance, we generated a library of Mycobacterium smegmatis mc 2 155 transposon insertion mutants. Upon screening this library, we identified one mutant that was hypersensitive to rifampin. The transposon insertion was localized to the arr gene, which encodes rifampin ADP ribosyltransferase, an enzyme able to inactivate rifampin. Sequence analysis revealed differences in the arr alleles of M. smegmatis strain mc 2 155 and previously described strain DSM 43756. The arr region of strain mc 2 155 contains a second, partial copy of the arr gene plus a novel insertion sequence, IS1623.Mycobacterial infections, including tuberculosis (TB) and leprosy, are bacterial diseases of global importance. The World Health Organization estimates that the worldwide incidences of TB increased 0.4% in 2001, to 8.5 million new cases (28). Control of TB is complicated by its ease of transmission, difficulties in administering the long-course chemotherapy regimens, and the appearance of strains that are multidrug resistant (MDR), which is defined as resistance to the two front-line anti-TB drugs, isoniazid and rifampin. Rifampin is a broad-spectrum antibiotic that inhibits bacterial DNA-dependent RNA polymerase activity. Resistance to rifampin is most often caused by mutations in rpoB, which encodes the ␤ subunit of RNA polymerase (20,25). In rifampin-resistant clinical isolates of Mycobacterium tuberculosis, an estimated 96% of the rpoB mutations map to an 81-bp region (codons 507 to 533) near the middle of the gene (20). Mutations at codons 531, 526, and 516 are the most common (13). A variety of assays that detect these sequence polymorphisms have been developed (3, 23) and allow the rapid determination of the drug susceptibilities of clinical M. tuberculosis isolates. In addition, an estimated 90% of rifampin-resistant clinical isolates are also isoniazid resistant, such that rifampin resistance is a positive indicator of MDR TB. However, about 4% of rifampin-resistant clinical isolates of M. tuberculosis have no mutations in the 81-bp core region or elsewhere in the rpoB gene (3,8,14,20). In addition, some mycobacteria, particularly atypical mycobacteria, such as Mycobacterium smegmatis, Mycobacterium avium, and Mycobacterium intracellulare, are resistant to rifampin, even though they possess sensitive RNA polymerase (9,14,20). These findings indicate that other genes can contribute to rifampin resistance. To identify s...

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Section: Discussionmentioning

confidence: 99%

A Rifampin-Hypersensitive Mutant Reveals Differences between Strains of Mycobacterium smegmatis and Presence of a Novel Transposon, IS 1623

Alexander

Jones

Liu

2003

Antimicrob Agents Chemother

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“…Vol. 57 (2011) lates each collected from a single patient as a clinical specimen: urine (446), blood (45), pus (40), body fluid (30), sputum (16), and others (20) 25) All intermediate susceptible strains were considered to be resistant strains. The presence of the ESBL phenotype was initially screened using four antimicrobial disks containing CTX (30 µg), CRO (30 µg), ceftazidime (30 µg) and cefpodoxime (10 µg).…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12][13][14][15][16][17][18][19] However, there have been only a few reports on ESBL-producing bacteria isolated in Thailand. 5,[20][21][22][23] Our objective for this study was to determine the prevalence of resistance factors, including the production of ESBL and the presence of class 1 integrons, in E. coli isolated from clinical specimens obtained at the Songklanagarind Hospital the major university hospital in southern Thailand. Vol.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance, Class 1 Integrons and Extended-Spectrum .BETA.-Lactamases in Escherichia coli Clinical Isolates from Patients in South Thailand

Phongpaichit

Tunyapanit

Pruekprasert

2011

JOURNAL OF HEALTH SCIENCE

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Five hundred and ninety seven Escherichia coli (E. coli) isolates were obtained from clinical specimens at the Songklanagarind Hospital in Songkhla Province, Thailand during [2003][2004][2005]. Antimicrobial susceptibilities to ten antimicrobial agents were tested by a standard disk diffusion method. The presence of class 1 integrons was based on the detection of the integrase gene (intI1) by PCR. Extended-spectrum β-lactamases (ESBLs) were detected by a combination disk method. The highest percentage of resistance was found to ciprofloxacin (40.5%), norfloxacin (39.0%), and cefuroxime (33.2%). The IntI1 was detected in 59.5% of the tested isolates. Resistance to gentamicin, cefazolin, cefuroxime, cefotaxime, ceftriaxone, norfloxacin, and ciprofloxacin was significantly higher in class 1 integron-positive isolates (p < 0.05). The most prominent resistance pattern was for norfloxacinciprofloxacin (17.7%). ESBLs were detected in 75 out of 597 (12.6%) isolates; 56/302 (18.5%) and 19/295 (6.4%) were from hospitalized and non-hospitalized patients, respectively. Seventy-five percent of ESBL-positive strains were integron-positive isolates. Imipenem and meropenem were still able to inhibit all ESBL-producing strains. The results indicated that class 1 integrons are widely prevalent among clinical isolates of resistant E. coli especially in ESBL-producers and are probably a reservoir for producing multidrug resistance and nosocomial infections in hospitals.

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“…9,10 This suggests the interspecies horizontal gene transfer and international dissemination of this mobile genetic element among international clones of Enterobacteriaceae and minor variation brought about by selective antibiotic pressure in different geographical areas. 16 …”

Section: Discussionmentioning

confidence: 99%

Class 1 integron element in Thai Acinetobacter baumannii reveals a linkage to the European clone I

Thapa¹,

Tribuddharat

Srifuengfung

et al. 2012

Int J Infection & Microbiol

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BACKGROUND: Class 1 integron element is innate to most of the multidrug resistant Acinetobacter baumannii and its spread is common among international clones worldwide. The aim of this study was to document the presence of blaVEB-1 harboring class 1 integron element and its gene cassettes in Thai A. baumannii in relation to A. baumannii European clone I, AYE strain. MATERIALS AND METHODS: Thirty seven carbapenem resistant A. baumannii isolates identified in routine microbiology laboratory of Siriraj Hospital, Bangkok were studied. The dot blot hybridization was performed to detect class 1 integron element integrase gene. PCR was used to amplify blaVEB-1, arr2, cmlA, blaOXA-10 resistance cassettes, and variable region of class 1 integron element. blaVEB-1 gene was localized by southern blot hybridization. RESULTS: The prevalence of class 1 integron element was 86.48% in the isolates studied. The blaVEB-1 was present in 7 isolates however the location of blaVEB-1 gene was different in different isolate. Four isolates (Ab03-168, Ab04-28, Ab08-20, and Ab08-22) harbored calss 1 integron element variable region sized 5.5 kb as described in strain AYE. However, blaVEB-1 was only amplified from Ab03-168. The cassette organization in this isolate was 5’CS-aadB-blaVEB-1-arr2-cmlA-blaOXA- 10-aadA1-3’CS. The class 1 integron element similar to the element identified in genomic resistance island, AbaRI of European clone I, AYE was identified in Thai A. baumannii. CONCLUSIONS: blaVEB-1 harboring class 1 integron element with minor cassette variation was identified in Thai A. baumanni isolate which might suggest the spread of this resistant cassette or the spread of the European clone I in Thailand. Monitoring of the global spread of multi-resistant A. baumannii is mandatory to control the spread of resistant genes and this multi-resistant pathogen. DOI: http://dx.doi.org/10.3126/ijim.v1i1.6715Int J Infect Microbiol 2012;1(1):24-28

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Molecular Epidemiology of the Integron-Located VEB-1 Extended-Spectrum β-Lactamase in Nosocomial Enterobacterial Isolates in Bangkok, Thailand

Cited by 126 publications

References 46 publications

A Rifampin-Hypersensitive Mutant Reveals Differences between Strains of Mycobacterium smegmatis and Presence of a Novel Transposon, IS 1623

A Rifampin-Hypersensitive Mutant Reveals Differences between Strains of Mycobacterium smegmatis and Presence of a Novel Transposon, IS 1623

Antimicrobial Resistance, Class 1 Integrons and Extended-Spectrum .BETA.-Lactamases in Escherichia coli Clinical Isolates from Patients in South Thailand

Class 1 integron element in Thai Acinetobacter baumannii reveals a linkage to the European clone I

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