Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil

Santos, Jucélia Stadinicki dos; Alfieri, Alice Fernandes; Leite, José Paulo Gagliardi; Skraba, Irene

doi:10.1590/s1516-89132008000200008

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…[28][29][30] The electropherotypes of these strains of RV-A showed a slight predominance of short pattern in comparison to the long-pattern strains, albeit, unusual, this has been reported in India. 31 In general, the predominance of long e-types has been reported throughout the world, [32][33][34][35][36] however, variation has been shown according to some genotypes. As far as VP7 genotyping is concerned, our study revealed greater incidence of G2 secondly by G1.…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of human rotavirus in hospitalized patients in the cities of Ponta Grossa, Londrina and Assai - PR, Brazil

Nozawa¹,

Kerntopf²,

Czernisz³

et al. 2010

Braz J Infect Dis

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Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under fi ve years of age are the target population. Objective: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai -Paraná. Methods: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. Result: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of them being RV group A (RV-A). Overall, most of the RV positive stool samples came from children under thirteen years of age. However, 12 positive cases occurred in patients aged 13 years or above, including an 81-year old patient. Conclusion: The data showed similar electropherotypes and genotypes G, P and NSP4 of the inland wild circulating strains of RV.

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