Molecular epidemiology of methicillin resistant staphylococcus aureus colonizing the anterior Nares of school children of Udupi Taluk

Govindan, Sreejith; Maroli, Amith S.; Ciraj, AM; Bairy, Indira

doi:10.4103/0255-0857.150919

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Another very similar project to ours was conducted in Argentina, where 316 healthy children were screened in a given city, and here again all 14 MRSA strains carried the SCCmec type IV cassette [26]. On the other hand, in India, where 1,503 school-age children were screened, only 7 out of the 17 MRSAs belonged to type IV and none were identified as type V either [27]. The MLST typing revealed that all MRSA isolates in this study belonged to ST45, a clone which has been very frequently reported from numerous countries from all over the world, based on the MLST database [9].…”

Section: Discussionsupporting

confidence: 61%

High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary

Laub

Tóthpál

Kovács

et al. 2017

AMicr

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We collected nasal samples from 1,390 healthy 3-7 years old children in Szolnok city, Hungary, in 2012. We detected 476 Staphylococcus aureus isolates from 474 children. In two occasions, two different S. aureus were isolated, based on hemolysis type and pulsed-field gel electrophoresis pattern. S. aureus carriage rate was calculated to be 34.1% similar to others studies. Male gender was found to be a risk factor for carriage by statistical analysis. Altogether, four methicillin-resistant S. aureus (MRSA) strains were detected by mecA polymerase chain reaction, which means 0.8% community-acquired MRSA prevalence among the S. aureus isolates. All MRSA strains harbored the SCCmec type IV cassette (typical for CA-MRSA) and belonged to ST45 by multilocus sequence typing. During antibiotic susceptibility testing, we measured the following resistance rates: 0.0% for mupirocin, 0.2% for ciprofloxacin, 0.6% for gentamicin and oxacillin, 3.4% for tetracycline, 9.5% for clindamycin, 10.3% for erythromycin, and 91.4% for penicillin, which are generally lower compared with Hungarian clinical isolates.

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Section: Discussionsupporting

confidence: 61%

High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary

Laub

Tóthpál

Kovács

et al. 2017

AMicr

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“…The carriage of pvl gene (pvl+) in 44.7% of MRSA isolates in this report is consistent with 58.8% reported by Govindan et al (37) and in dissidence with reports from Nigeria (16%) (38), Egypt (2.2%) (39), Uganda (73%) (40). In our study, among the tested mecA+ MRSA isolates, pvl+ was chiefly associated with HA genotype (37.5%).…”

Section: Discussionsupporting

confidence: 91%

Constellation of methicillin-resistant genomic islands (SCCmec) among nasal meticillin-resistant Staphylococcus aureus isolates

Suleiman¹,

Ademola²,

Olalekan³

2021

Acta fac medic Naissensis

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The apprehensiveness for the knowledge vacuum on existential threat of nasal carriage of pvl+ healthcare-acquired meticillin-resistant Staphylococcus aureus (HA-MRSA) strains amongst subjects in hospitals have led us to pursue a grasp on the constellation of staphylococcal cassette chromosome mec (SCCmec) types and pvl gene among mecA positive MRSA nasal strains. This was accomplished by phenotypic (catalase, coagulase, Microgen staph ID, ORSAB) and genotypic (polymerase chain reaction) biotyping techniques. All the mecA+ strains harboured the SCCmec gene; SCCmec type I prevailed in 43.75% and pvl was found in 42.1% of the isolates. Dual carriage of mecA and pvl genes occurred in six (37.5%, n = 6/16) strains. Overall, majority of the mecA+ MRSA strains documented in this study carried SCCmec elements of the HA genotype with a hint of community-acquired (CA)genotype suggesting a possible coexistence of both HA-MRSA and community-acquiredhealthcare-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains. Consequently, the implementation of methodical surveillance is needed for the evaluation of potential shifts in directionality of (HA-MRSA/CA-MRSA) pvl+ MRSA clones in our hospitals for effective and prudent antimicrobial stewardship.

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“…A soothing fact to be noted is the absence of such multi-drug resistant MRSA in their study settings. This indicates that neither HA-MRSA has invaded into community settings nor CA-MRSA has acquired multi-drug resistance genes ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Typing of Methicillin Resistant Staphylococcus Aureus Using DNA Fingerprints by Pulsed-field Gel Electrophoresis

et al. 2016

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Background:Methicillin resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to analyze MRSA strains from the general population in Canton Sarajevo, B&H.Methods:Our investigation including either phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and Panton-Valentine leukocidin (PVL) detection.Results:Antimicrobial susceptibility: all MRSA isolates were resistant to the β-lactam antibiotics tested, and all isolates were susceptible trimethoprim sulphamethoxazole, rifampicin, fusidic acid, linezolid and vancomycin. Sixty-eight per cent of the MRSA isolates were resistant to erythromycin, 5% to clindamycin, 5% to gentamicin and 4% to ciprofloxacin. After the PFGE analysis, the isolates were grouped into five similarity groups: A-E. The largest number of isolates belonged to one of two groups: C: 60 (60%) and D: 27 (27%). In both groups C and D, SCCmec type IV was predominant (60% and 88, 8%, respectively). A total of 24% of the isolates had positive expression of PVL genes, while 76% showed a statistically significantly greater negative expression of PVL genes.Conclusion:SCCmec type IV, together with the susceptibility profile and PFGE grouping, is considered to be typical of CA-MRSA

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Molecular epidemiology of methicillin resistant staphylococcus aureus colonizing the anterior Nares of school children of Udupi Taluk

Abstract: The Indian variant EMRSA-15 gains much epidemiological relevance owing to the acquisition of pvl gene. In spite of low prevalence of nasal colonization of MRSA, emergence of the virulent Indian variant EMRSA-15 in our community is a worrisome fact to be reckoned with.

Cited by 6 publications

References 21 publications

High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary

High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary

Constellation of methicillin-resistant genomic islands (SCCmec) among nasal meticillin-resistant Staphylococcus aureus isolates

Typing of Methicillin Resistant Staphylococcus Aureus Using DNA Fingerprints by Pulsed-field Gel Electrophoresis

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