Molecular epidemiology of Korean porcine sapeloviruses

Son, Kyu-Yeol; Kim, Deok-Song; Matthijnssens, Jelle; Kwon, Hyoung-Jun; Park, Jun-Gyu; Hosmillo, Myra; Alfajaro, Mia Madel; Ryu, Eun-Hye; Kim, Jiyun; Kang, Mun-Il; Cho, Kyoung-Oh

doi:10.1007/s00705-013-1901-6

Cited by 18 publications

(29 citation statements)

References 23 publications

(24 reference statements)

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“…SV-A strain KS04105 used in this study was isolated from faecal samples of diarrhoeic piglets from South Korea (Son et al , 2014a, b). This strain was passaged eight times in LLC-PK cells, including isolation, adaptation and triple plaque purification.…”

Section: Methodsmentioning

confidence: 99%

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Pathogenesis of Korean Sapelovirus A in piglets and chicks

Kim

Kang

Son

et al. 2016

Journal of General Virology

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Sapelovirus A (SV-A), formerly known as porcine sapelovirus as a member of a new genus Sapelovirus, is known to cause enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. We have recently identified α2,3-linked sialic acid on GD1a ganglioside as a functional SV-A receptor rich in the cells of pigs and chickens. However, the role of GD1a in viral pathogenesis remains elusive. Here, we demonstrated that a Korean SV-A strain could induce diarrhoea and intestinal pathology in piglets but not in chicks. Moreover, this Korean SV-A strain had mild extra-intestinal tropisms appearing as mild, non-suppurative myelitis, encephalitis and pneumonia in piglets, but not in chicks. By real-time reverse transcription (RT) PCR, higher viral RNA levels were detected in faecal samples than in sera or extra-intestinal organs from virus-inoculated piglets. Immunohistochemistry confirmed that high viral antigens were detected in the epithelial cells of intestines from virus-inoculated piglets but not from chicks. This Korean SV-A strain could bind the cultured cell lines originated from various species, but replication occurred only in cells of porcine origin. These data indicated that this Korean SV-A strain could replicate and induce pathology in piglets but not in chicks, suggesting that additional porcine-specific factors are required for virus entry and replication. In addition, this Korean SV-A strain is enteropathogenic, but could spread to the bloodstream from the gut and disseminate to extra-intestinal organs and tissues. These results will contribute to our understanding of SV-A pathogenesis so that efficient anti-sapelovirus drugs and vaccines could be developed in the future.

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Section: Methodsmentioning

confidence: 99%

“…This strain was passaged eight times in LLC-PK cells, including isolation, adaptation and triple plaque purification. Isolated viruses were confirmed as PSVs based on immunofluorescence assay (IFA), RT-PCR and transmission electron microscopy (Kim et al , 2016; Son et al , 2014a, b). …”

Section: Methodsmentioning

confidence: 99%

Pathogenesis of Korean Sapelovirus A in piglets and chicks

Kim

Kang

Son

et al. 2016

Journal of General Virology

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“…A recent Spanish study has demonstrated that SV-A is detected in the fecal samples from asymptomatic pigs and its infection has an age predilection to postweaning pigs [10, 14, 15]. In Korea, SV-A infections are common in pigs with diarrhea [12]. However, the occurrence and genetic diversity of SV-As in fecal samples from asymptomatic pigs and age predilection in both diarrhea and asymptomatic pigs are largely unknown.…”

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confidence: 99%

“…To determine the exact occurrence of SV-A infections across all age groups and their association with diarrhea, 110 fecal specimens from diarrheic or non-diarrheic pigs from previously SV-A positive farm [12] were collected during the winter of 2011 (Table 1…”

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confidence: 99%

“…of collected samples [diarrheic/non-diarrheic]) tested were as follows: suckling (≤3 weeks; n=30 [15/15]), weaned (≤8 weeks; n=20 [10/10]), grower (≤20 weeks; n=20 [10/10]), finisher (≥20 weeks; n=20 [10/10]) and sows (≥1 year; n=20 [10/10]). To detect SV-A RNA in each fecal sample, RT-nested PCR assays with primer pairs specific to VP1 or 2C regions were performed with extracted RNA [12]. In addition, other common enteric pathogens including viruses and bacteria were screened by RT-nested PCR, PCR or specific agar media [12].…”

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Occurrence and molecular characterization of <i>Sapelovirus A</i> in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm

Bak

Kang

Son

et al. 2016

The Journal of Veterinary Medical Science

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To determine the occurrence and genetic diversity of Sapelovirus A (SV-A) in diarrhea and non-diarrhea feces of Korean pigs, 110 specimens from different age groups of pigs in the same farm were analyzed by RT-nested PCR. SV-As were detected in 60% of both diarrhea and non-diarrhea specimens regardless of age groups with primer pairs for 2C region, in which all diarrhea samples were co-infected by other enteric pathogens. Phylogenetical analysis of partial VP1 region showed that our strains and several other Korean strains belonged to cluster I, distinct from some strains reported in Korea and other countries. These data indicate that genetically distinct SV-As are frequently detected in Korean pigs irrespective of diarrhea and age.

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First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China

Yang

Wang

et al. 2017

Arch Virol

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Outbreaks of diarrhea in piglets cause serious economic consequences in China. Diarrhetic fecal samples from 20 Hunan farm piglets were tested and found to be positive for porcine epidemic diarrhea virus (PEDV) by RT-PCR, although incubation with porcine kidney (PK-15) cells failed to produce infectious PEDV. Four porcine sapelovirus (PSV) strains (designated as PSV-HuNs) were isolated from four of the samples. Genomic sequence analysis revealed open reading frames encoding polyproteins of 2,331 (HuN1, 2 and 3) and 2,332 (HuN4) amino acids. Homology comparisons of the VP1 gene of the four Hunan strains with previously reported PSV strains revealed nucleotide sequence identities ranging from 74.2 to 98.6%, and deduced amino acid sequence identities from 79.5 to 98%. Phylogenetic analyses based on full-length and partial VP1 gene sequences showed that 3 of the PSV-HuN strains (HuN2, 3 and 4) clustered within a clade distinct from HuN1 as well as from all PSVs previously isolated in China, thereby showing that genetic diversity exists within Chinese PSVs. In addition, recombination analysis among PSVs indicates that a recombinant (HuN2 strain) exist in China.

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Molecular epidemiology of Korean porcine sapeloviruses

Cited by 18 publications

References 23 publications

Pathogenesis of Korean Sapelovirus A in piglets and chicks

Pathogenesis of Korean Sapelovirus A in piglets and chicks

Occurrence and molecular characterization of <i>Sapelovirus A</i> in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm

First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China

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