Occurrence and molecular characterization of <i>Sapelovirus A</i> in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm

Abstract

To determine the occurrence and genetic diversity of Sapelovirus A (SV-A) in diarrhea and non-diarrhea feces of Korean pigs, 110 specimens from different age groups of pigs in the same farm were analyzed by RT-nested PCR. SV-As were detected in 60% of both diarrhea and non-diarrhea specimens regardless of age groups with primer pairs for 2C region, in which all diarrhea samples were co-infected by other enteric pathogens. Phylogenetical analysis of partial VP1 region showed that our strains and several other Korean strains belonged to cluster I, distinct from some strains reported in Korea and other countries. These data indicate that genetically distinct SV-As are frequently detected in Korean pigs irrespective of diarrhea and age.

Detection and Characterization of Porcine Sapelovirus in Italian Pig Farms

“…Our results indicate that PSV is largely widespread especially among younger animals confirming previous findings observed in Korea [15] Spain [14], the Czech Republic [11], and Hungary [9]. In general, circulation in older animals is not detected or occurs less frequently (Spain and the Czech Republic) [11,14].…”

“…The virus has also been found in wild boars with a prevalence of 6.4% in Spain [10] and 27.8% in the Czech Republic [11]. Co-infection of PSV with other enteric viral pathogens (e.g., Porcine teschovirus, PTV; Porcine Enterovirus, PEV) is frequently reported in both asymptomatic animals or in association with symptoms but information on its role in co-infections is still unavailable [5,8,[10][11][12][13][14][15].…”

First Identification and Genomic Characterization of a Porcine Sapelovirus from Corsica, France, 2017

“…PSV is transmitted by the fecal-oral route, and infection can be asymptomatic or associated with diarrhea, respiratory distress, encephalitis, skin lesions, and reproductive disorders ( 1 – 3 ). The virus has been detected worldwide in Asia (China [ 2 ], India [ 1 ], and South Korea [ 4 , 5 ]), the Americas (United States [ 6 , 7 ] and Brazil [ 8 ]), and Europe (Germany, United Kingdom [ 9 ], and Spain [ 10 ]).…”

“…KJ821019 , KX574284 , LC326555 , JX286666 , and KF539414 ). According to current knowledge ( 4 , 6 , 13 ), the ORF encodes four structural proteins (VP4, VP3, VP2, and VP1) and seven nonstructural proteins (2A, 2B, 2C, 3A, 3B, 3C, and 3D). The strain was assigned the name PSV OPY-1-Corsica-2017 and made available in the European Virus Archive collection (and under GenBank accession no.…”

Complete Genome Sequencing of a Novel Strain of Sapelovirus A Circulating in Vietnam

“…S apelovirus A (SV-A) is a nonenveloped single-stranded positive-sense RNA virus belonging to the family Picornaviridae, genus Sapelovirus. SV-A may cause acute diarrhea, respiratory and reproductive disorders, and polioencephalomyelitis in pigs, although the infection is most frequently asymptomatic (1)(2)(3)(4). SV-A infects mainly through the oral route, replicates in the gastrointestinal epithelia, and is excreted into feces, which are a source for secondary infections (5).…”