Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil

Soares, Luana da Silva; Lima, A. F.; Pantoja, Kamilla C; Lobo, Patrícia dos Santos; Cruz, Jonas França da; Guerra, Sylvia de Fátima dos Santos; Bezerra, Delana Andreza Melo; Bandeira, Renato Silva; Mascarenhas, Joana D’Arc Pereira

doi:10.1099/jmm.0.001026

Cited by 12 publications

(21 citation statements)

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“…Our results corroborate with data from few studies conducted in Brazil that have investigated HBoV in stool samples from AGE cases. Recently, two studies with hospitalized children presenting AGE symptoms from Northern Brazil reported HBoV detection rates of 24% [21] and 12.2% [29]. Albuquerque et al [30] found HBoV in 2% of stool samples (n = 705) from inpatient and outpatient children with AGE symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…This high percentage could be associated to multiple gastroenteric viruses tested in our study—RVA, HAdV, norovirus GI and GII, sapovirus and astrovirus. Previously in Brazil, Soares et al [ 21 ] identified co-infections between HBoV and RVA in 50% of cases. In China, two studies described co-infections of HBoV with either RVA or norovirus in 64% of cases [ 37 ], and in 77.6% of cases co-infected with RVA, norovirus, astrovirus, or enteric HAdV [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Southeastern Brazil, Santos et al [ 62 ] firstly demonstrated the circulation of HBoV-2 (20.8%) and HBoV-3 (0.6%) in stool samples from patients with AGE. More recently, in Northern Brazil, two studies have reported the circulation of HBoV-1, 2 and 3 in stool samples from children hospitalized for AGE, and both found a predominance of genotype 1 (94.8% and 75%) [ 21 , 29 ]. In line with our findings, studies performed elsewhere have predominantly detected genotypes 1–3 in AGE cases [ 13 , 63 , 64 , 65 ], and HBoV-4 has been rarely detected, or detected in lower prevalence compared to other genotypes [ 12 , 39 , 52 , 60 , 63 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, based on the genomic analysis of the two major structural proteins (VP1-2), HBoV is divided into four species or genotypes (HBoV-1 to 4) [12]. HBoV genotypes are globally distributed, and have been single or co-detected in AGE cases, with incidence varying from 0.5 to 25%, especially in children younger than two years old [10,16,[19][20][21].…”

Section: Of 14mentioning

confidence: 99%

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Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

et al. 2020

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Human bocavirus (HBoV) is an emerging virus and has been detected worldwide, especially in pediatric patients with respiratory and gastrointestinal infection. In this study, we describe HBoV prevalence, genotypes circulation and DNA shedding, in stool samples from children up to two years of age in Brazil. During 2016 and 2017, 886 acute gastroenteritis (AGE) stool samples from ten Brazilian states were analyzed by TaqMan®-based qPCR, to detect and quantify HBoV. Positive samples were genotyped by sequencing the VP1/2 overlap region, followed by phylogenetic analysis and co-infections were accessed by screening other gastroenteric viruses. HBoV was detected in 12.4% (n = 110) of samples, with viral load ranging from 1.6 × 102 to 1.2 × 109 genome copies per gram of stool. From these, co-infections were found in 79.1%, and a statistically lower HBoV viral load was found compared to viral loads of rotavirus, norovirus and adenovirus in double infected patients (p < 0.05). No significant differences were found between HBoV viral load in single or co-infections, age groups or genotypes. Phylogenetic analysis identified the circulation of HBoV-1 in 38%, HBoV-2 in 40% and HBoV-3 in 22%. Continuous HBoV monitoring is needed to clarify its role in diarrhea disease, especially in the absence of classic gastroenteric viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Of 14mentioning

confidence: 99%

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Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

et al. 2020

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“…Genome of HBoV consists of an approximately 5.5 kb linear single-stranded DNA, possesses three open reading frames (ORFs): ORF1 encoding non-structural protein NS1, ORF2 encodes nuclear phosphoprotein NP1 and ORF3 encodes viral capsid proteins VP1/VP2 [4]. Human Bocavirus is the second human pathogen parvovirus and divided into four species; HBoV-1 were predominantly identified in respiratory samples whereas the three other types, HBoV-2, HBoV-3 and HBoV-4, have been detected in fecal samples and described as enteric viruses [5]. Human Bocavirus can be transmitted through direct contact with infected faces (faecal-oral-route), ingestion of contaminated food/water, person-to-person contact and poor personal hygiene [6].…”

Section: Introductionmentioning

confidence: 99%

Detecting and Genotyping of Human Bocavirus among Children with Gastroenteritis in Diyala Governorate

Ali¹,

Hussein²,

kadim³

et al. 2020

ATMPH

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Human Bocavirus a newly detected parvovirus initially described as a respiratory pathogen, to be a possible causative agent of gastroenteritis in children. This cross-sectional study was carried out for patients with acute gastroenteritis who present in the Emergency Department of Pediatrics in Al-Batool Teaching Hospital for Maternity and Children in Baqubah city, during the period from July 2019 to March 2020. The study population consists of 100 children with diarrhea collected from children less than five years old (58 males, 42 females) to detect human Bocavirus by conventional polymerase chain reaction. Human Bocavirus was detected in 9 samples (9%), high infection observed in males (66.66%) and in age group 6-12 months (55.55%). Most infection occur in children whose mothers were illiterates and primary education with equal percentage (44.44%). The genetic analysis of the sequence of four samples (4 out of 9 positive case) showed three of them belong to HBoV-3,whereas the fourth belong to HBoV-2.

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Prevalence, molecular characterization, and clinical features of human bocavirus in children under 5 years of age with acute gastroenteritis admitted to a specialized children's hospital in Iran: A cross‐sectional study

Kachooei,

Karbalaie Niya,

Khales

et al. 2023

Health Science Reports

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Background and AimsAlthough some reports have confirmed the role of human bocavirus (HBoV) in respiratory infections, the importance of this virus in causing acute gastroenteritis has not yet been proven. This study aimed to determine the molecular prevalence of HBoV in children under 5 years old with gastroenteritis and to compare the clinical symptoms of HBoV‐positive and ‐negative gastroenteritis cases.MethodsA total of 100 stool samples were collected from children with gastroenteritis hospitalized in a pediatric hospital in Tehran, Iran. Demographic and clinical data were collected from patients' medical records. Viral genomic DNA was extracted from stool samples and amplified using the PCR assay. Finally, sequencing was used to determine the genotype of HBoV.ResultsThe HBoV genome was detected in 14 samples (14%). The highest prevalence of HBoV was observed in the age range of 24–60 months (n = 5; 35.7%); However, no statistically significant relationship was observed between the prevalence of HBoV and age groups (p = 0.09). Nine (64.3%) and 5 (35.7%) HBoV‐positive cases were boys and girls, respectively (p = 0.45). Fever, vomiting, and heartache were seen in 5 (35.7%), 3 (21.4%), and 1 (7.1%) HBoV‐positive patients, respectively. Overall, no significant difference was observed in any of the investigated clinical manifestations between patients positive or negative for HBoV. Five HBoV‐positive samples were subjected to sequencing and all five sequenced samples were genotype 3.ConclusionHBoV infections can be considered a risk factor for causing at least a portion of acute gastroenteritis cases in children under 5 years of age.

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Molecular epidemiology of human bocavirus in children with acute gastroenteritis from North Region of Brazil

Cited by 12 publications

References 30 publications

Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

Detecting and Genotyping of Human Bocavirus among Children with Gastroenteritis in Diyala Governorate

Prevalence, molecular characterization, and clinical features of human bocavirus in children under 5 years of age with acute gastroenteritis admitted to a specialized children's hospital in Iran: A cross‐sectional study

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