Molecular Epidemiology of HIV in Brazil: Polymorphism of the Antigenically Distinct HIV-1 B Subtype Strains

Masucci, Maria G.; Guimarães, Monick Lindenmeyer; Neves, Ivan; Santos, VG Veloso dos; Linhares-de-Carvalho, MI; Lr, Castello-Branco; Ea, Castilho; Galvão–Castro, Bernardo; Bongertz,

doi:10.1590/s0074-02761998000300020

Cited by 23 publications

(17 citation statements)

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“…Moreover, the prevalence of subtype F was quite low in the present study (4.9%), even including the B/F recombinant forms (8.1%). According to previous results of our laboratory (Morgado et al 1998) and others (Brindeiro et al 2003) the prevalence of this subtype in Rio de Janeiro varied around 15%, based on studies of drug naïve HIV-1 positive individuals within distinct exposure categories. If these differences could be attributed to differential HIV-1 subtype ARV-drug susceptibility has to be further addressed.…”

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confidence: 52%

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Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy

Couto-Fernandez

Jesus

Veloso

et al. 2005

Mem. Inst. Oswaldo Cruz

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In order to assess the human immunodeficiency virus type 1 (HIV-1 .9%), and B/F viral recombinant forms (3.3%). The subtype C was identified in two patients (0.4%) and the recombinant CRF_02/AG virus was found infecting one patient (0.2%). The HIV-1 genotyping profile associated to the reverse transcriptase inhibitors has shown a high frequency of the M184V mutation followed by the timidine-associated mutations. The K103N mutation was the most prevalent to the nonnucleoside RT inhibitor and the resistance associated to protease inhibitor showed the minor mutations L63P, L10F/R, and A71V as the more prevalent.A

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confidence: 52%

“…A large proportion of HIV-1 subtype B infections was observed in HAART failing patients from Rio de Janeiro, higher than had been previously described for this subtype in the region (Morgado et al 1998, Bongertz et al 2000, Brindeiro et al 2003. Indeed, we have found that almost 92% of the virus circulating in the state belongs to subtype B.…”

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confidence: 77%

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy

Couto-Fernandez

Jesus

Veloso

et al. 2005

Mem. Inst. Oswaldo Cruz

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“…Restriction fragment length polymorphism (RFLP) was used to identify B and B" HIV-1 subtypes 15 . PCR amplification of env and pol regions was performed as previously described 16,17 , and the products were direct-sequenced by an automated fluorescent method (ABI DNA Sequencer 310 or 3100).…”

Section: • Molecular Studiesmentioning

confidence: 99%

HIV-1 seroprevalence in the general population of Salvador, Bahia State, Northeast Brazil

et al. 2007

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“…The M group is further subdivided into 11 subtypes (A-K) and 14 circulating recombinant forms (CRFs) (18,20,29). Such diversity has potential implications for vaccine design as well as for diagnostics, pathogenesis and epidemiology (19).…”

Section: Introductionmentioning

confidence: 99%

HIV-1 subtypes and mutations associated to antiretroviral drug resistance in human isolates from Central Brazil

Cerqueira¹,

Ramalho²,

Oliveira³

et al. 2004

Braz. J. Microbiol.

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The detection of polymorphisms associated to HIV-1 drug-resistance and genetic subtypes is important for the control and treatment of HIV-1 disease. Drug pressure selects resistant variants that carry mutations in the viral reverse transcriptase (RT) and protease (PR) genes. For a contribution to the public health authorities in planning the availability of therapeutic treatment, we therefore described the genetic variability, the prevalence of mutations associated to drug resistance and the antiretroviral resistance profile in HIV-1 isolates from infected individuals in Central Brazil. Nineteen HIV-1 RNA samples from a Public Health Laboratory of the Federal District were reversely transcribed and cDNAs were amplified by nested PCR. One fragment of 297 bp coding the entire protease gene, and another of 647 bp, corresponding to the partial RT gene (codons 19-234), were obtained. Automated sequencing and BLAST analysis revealed the presence of 17 B and 2 F1 HIV-1 subtypes. The amino acid sequences were analyzed for the presence of resistance-associated mutations. A total of 6 PR mutations, 2 major and 4 accessory, and 8 RT mutations related to drug resistance were found. Our data suggest a high prevalence of HIV-1 B subtype in the studied population of Federal District as well as the presence of genetically-resistant strains in individuals failing treatment.

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Molecular Epidemiology of HIV in Brazil: Polymorphism of the Antigenically Distinct HIV-1 B Subtype Strains

Cited by 23 publications

References 0 publications

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy

Human immunodeficiency virus type 1 (HIV-1) genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing highly active antiretroviral therapy

HIV-1 seroprevalence in the general population of Salvador, Bahia State, Northeast Brazil

HIV-1 subtypes and mutations associated to antiretroviral drug resistance in human isolates from Central Brazil

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