Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin

Zohoncon, Théodora Mahoukèdè; Ouedraogo, Teega-Wendé Clarisse; Brun, Luc; Obiri‐Yeboah, Dorcas; Djigma, Wendkuuni Florencia; Salifou, K; Ouattara, S.; Gomina, Moutawakilou; Yonli, Albert Théophane; BAZIE, Bapio Valérie Elvira Jean Télesphore; Ouédraogo, Charlemagne; Lompo, Olga Mélanie; Akpona, Simon; Simporé, Jacques

doi:10.3923/pjbs.2016.49.56

Cited by 16 publications

(11 citation statements)

References 23 publications

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“…HPV 59 and 39 were not found. Similar studies on HPV conducted in Burkina Faso and Vietnam using the same amplification kit and the same device showed various and predominant genotypes other than 56 and 66 [5] , [6] , [7] , [18] , [19] , [20] .…”

Section: Discussionsupporting

confidence: 54%

“…(2016b) [19] on invasive cervical cancer had revealed a predominance of HPV 18, 31, 39, 16, 45, 35 and 58. In addition, two similar studies conducted in Benin and Burkina Faso in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer showed lack of HPV 16 and a predominance of oncogenic genotypes, 18, 45, 35, 52, 33, 51, 31, 58, 66, 68, 56, 59, 68 [18] , [19] . This classical difference in the distribution of genotypes in our study population should lead to a larger study with more representative sample, to determine if the frequency of HPV16 circulating in women is lower than other populations and to characterize the predominant oncogenic genotypes in the subregion and particularly in cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

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Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa

Ouedraogo

Zohoncon²,

Guigma

et al. 2018

Papillomavirus Research

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ObjectiveThis study was conducted to determine the prevalence and distribution of high-risk human papillomavirus (HR-HPV) genotypes among sexually active women in Tenkodogo, Burkina Faso.MethodsAmong 131 sexually active women attending the Tenkodogo Urban Medical Center, endocervical samples were collected prior to screening for precancerous lesions. After viral DNA extraction, fourteen HR-HPV genotypes were characterized by real-time multiplex PCR in these cervical samples.ResultsThe mean age was 35.5 ± 9.5 years. Of the 131 women, 45 were infected with at least one HR-HPV genotype. The prevalence of HR-HPV infection among these women was 34.4%. Among the 45 oncogenic HPV-infected women, single HR-HPV genotype was found in 55.6% while 44.4% were infected with more than one HR-HPV genotype. The most frequent genotypes were HPV56 (36.5%), HPV66 (36.5%).ConclusionTenkodogo women included in this study had a higher prevalence of HPV 56, HPV 66. A larger study with a more representative sample would therefore be needed to determine predominant oncogenic genotypes in the subregion and especially in cancer cases.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa

Ouedraogo

Zohoncon²,

Guigma

et al. 2018

Papillomavirus Research

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“…HPV16 and HPV18 were the most common HPVs detected in this study and accounted for 39.6% and 19.8% respectively and 59.4% combined. These two high-risk HPVs are the most frequently studied and consequently implicated in the causation of cervical cancer worldwide [10, 34, 36-39]. They are the most prevalent and most potent carcinogenic viruses [10, 40].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Kabir¹,

Bukar²,

Nggada³

et al. 2019

Pan Afr Med J

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IntroductionCervical cancer is the commonest gynaecological malignancy and the second most common cancer among women worldwide. Several epidemiological, clinical and molecular studies have strongly implicated oncogenic high-risk human papillomavirus infection in the aetiopathogenesis of cervical cancer. The objectives of this study were to determine the cervical HPV prevalence and genotype distribution in cervical cancer in Maiduguri, Nigeria.MethodsThis was a descriptive and retrospective study. Sixty-three archived paraffin-embedded tissue blocks with confirmed diagnoses of cervical cancer during the study period (2013-2015) were retrieved and examined. The procedure included deparaffinization of tissue samples, DNA extraction, PCR, gel electrophoresis, and HPV genotyping by reverse hybridization line probe assay.ResultsSixty-three cervical cancer cases were subjected to genomic DNA extraction and HPV-DNA detection by PCR. Fifty-eight samples showed PCR positivity while 5 samples were PCR negative. HPV-specific DNA was detected in 44 of the 58 PCR-positive samples and thus the prevalence was 69.8%. Ten different high-risk HPV genotypes were detected. Both single and multiple high-risk HPV infections were observed. The most prevalent type of the human papillomavirus detected was HPV16.ConclusionHPV-DNA was prevalent in majority of the examined cervical cancer tissues and that HPV16, HPV18, HPV45, HPV51 and HPV52 were the predominant HPVs detected in both single and multiple HPV infections. The results of this study and further studies will provide more detailed information about HPV and may contribute significantly to the prevention of cervical cancer through primary high-risk HPV testing and HPV vaccination against the oncogenic viruses.

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“…Some studies have reported in Burkina Faso (Ouagadougou) other HR -HPV genotypes different from HPV 16 and HPV 18 (29.4% of HPV 35) [134, 137–140]. The situation seems to be the same in other countries of the subregion: Benin (Parakou) (38% of HPV 39) [141, 142]; Ivory Coast (Abidjan) (17.5% of HPV 35) [143]. HPV 16 is less frequent in Sub-Saharan Africa than in the rest of the world [144].…”

Section: Main Textmentioning

confidence: 99%

“…HPV 16 is less frequent in Sub-Saharan Africa than in the rest of the world [144]. One of the main genotypes targeted by vaccines HPV 16 has been declared absent during a study in Benin [141] while the two genotypes HPV 16 and 18 were marginal during a study in Burkina [145]. Multiple infections of HPV-HR were also observed in 78.03% of infected women [145].…”

Section: Main Textmentioning

confidence: 99%

Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

Bamogo

Brugidou

Sérémé

et al. 2019

Virol J

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BackgroundDeveloping African countries face health problems that they struggle to solve. The major causes of this situation are high therapeutic and logistical costs. Plant-made therapeutics are easy to produce due to the lack of the safety considerations associated with traditional fermenter-based expression platforms, such as mammalian cells. Plant biosystems are easy to scale up and inexpensive, and they do not require refrigeration or a sophisticated medical infrastructure. These advantages provide an opportunity for plant-made pharmaceuticals to counteract diseases for which medicines were previously inaccessible to people in countries with few resources.Main bodyThe techniques needed for plant-based therapeutic production are currently available. Viral expression vectors based on plant viruses have greatly enhanced plant-made therapeutic production and have been exploited to produce a variety of proteins of industrial, pharmaceutical and agribusiness interest. Some neglected tropical diseases occurring exclusively in the developing world have found solutions through plant bioreactor technology. Plant viral expression vectors have been reported in the production of therapeutics against these diseases occurring exclusively in the third world, and some virus-derived antigens produced in plants exhibit appropriate antigenicity and immunogenicity. However, all advances in the use of plants as bioreactors have been made by companies in Europe and America. The developing world is still far from acquiring this technology, although plant viral expression vectors may provide crucial help to overcome neglected diseases.ConclusionToday, interest in these tools is rising, and viral amplicons made in and for Africa are in progress. This review describes the biotechnological advances in the field of plant bioreactors, highlights factors restricting access to this technology by those who need it most and proposes a solution to overcome these limitations.

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Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin

Cited by 16 publications

References 23 publications

Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa

Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa

Prevalence of human papillomavirus genotypes in cervical cancer in Maiduguri, Nigeria

Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa

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