Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Punjab Province in Pakistan and a phylogenetic analysis

Aziz, Hafsa; Raza, Abida; Murtaza, Shahnaz; Waheed, Yasir; Khalid, Ali; Irfan, Javaid; Samra, Zahoor Qadir; Athar, Makshoof

doi:10.1016/j.ijid.2012.09.017

Cited by 33 publications

(28 citation statements)

References 23 publications

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“…The mortality rate because of hepatitis C virus (HCV) infection from primary liver diseases encompassing liver cirrhoses and hepatocellular carcinoma has been steadily increasing over the years [1,2]. HCV was identified in 1989, belonging to the family Flaviviridae and the genus Hepacivirus [3].…”

Section: Introductionmentioning

confidence: 99%

Determination of hepatitis C virus genotypes circulating in different districts of Punjab (Pakistan)

Waqar¹,

Khan²,

Rehman³

et al. 2014

European Journal of Gastroenterology & Hepatology

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The study found a significantly high prevalence of HCV among the patients of Punjab. In addition, genotype 1a was found to be a significantly and rapidly increasing genotype in the study area. It appears that HCV-3a (the most prevalent genotype) may be replaced by genotype 1a. If this occurs, it will make the present scenario more complex in terms of response to therapy and economic burden on the health sector of Pakistan. HCV infection is more common in the age group of 21-40 years. All the genotypes were distributed at the same frequency in men and women.

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Section: Introductionmentioning

confidence: 99%

Determination of hepatitis C virus genotypes circulating in different districts of Punjab (Pakistan)

Waqar¹,

Khan²,

Rehman³

et al. 2014

European Journal of Gastroenterology & Hepatology

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“…Out of seven studies from Iran, four studies were published in 2004 - 2014 (33-36) and three were unpublished. Furthermore, 15 published and unpublished studies from EMRO and Middle Eastern countries including Afghanistan (37), Azerbaijan, Cyprus (38, 39), Egypt, Morocco (40), Pakistan (41), and Tunisia (42, 43) were collected. Furthermore, other studies from the US and European countries were investigated, randomly (44-49).…”

Section: Resultsmentioning

confidence: 99%

Molecular Tracing of Hepatitis C Virus Genotype 1 Isolates in Iran: A NS5B Phylogenetic Analysis with Systematic Review

et al. 2016

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ContextHepatitis C virus (HCV) is characterized by a high degree of genetic heterogeneity and classified into 7 genotypes and different subtypes. It heterogeneously distributed through various risk groups and geographical regions. A well-established phylogenetic relationship can simplify the tracing of HCV hierarchical strata into geographical regions. The current study aimed to find genetic phylogeny of subtypes 1a and 1b of HCV isolates based on NS5B nucleotide sequences in Iran and other members of Eastern Mediterranean regional office of world health organization, as well as other Middle Eastern countries, with a systematic review of available published and unpublished studies.Evidence AcquisitionThe phylogenetic analyses were performed based on the nucleotide sequences of NS5B gene of HCV genotype 1 (HCV-1), which were registered in the GenBank database. The literature review was performed in two steps: 1) searching studies evaluating the NS5B sequences of HCV-1, on PubMed, Scopus, and Web of Science, and 2) Searching sequences of unpublished studies registered in the GenBank database.ResultsIn this study, 442 sequences from HCV-1a and 232 from HCV-1b underwent phylogenetic analysis. Phylogenetic analysis of all sequences revealed different clusters in the phylogenetic trees. The results showed that the proportion of HCV-1a and -1b isolates from Iranian patients probably originated from domestic sources. Moreover, the HCV-1b isolates from Iranian patients may have similarities with the European ones.ConclusionsIn this study, phylogenetic reconstruction of HCV-1 sequences clearly indicated for molecular tracing and ancestral relationships of the HCV genotypes in Iran, and showed the likelihood of domestic origin for HCV-1a and various origin for HCV-1b.

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“…Genotype-1, the most common genotype, it is dominant in USA, Europe and most Asia-Pacific [16]. Genotype-2 in Japan, South Korea and southern Taiwan [17,18]. Genotype-3 is prevalent on the Indian subcontinent and Australia.…”

Section: Virology Discovery Issn 2052-6202mentioning

confidence: 99%

The role of hepatitis C virus genotyping in evaluating the efficacy of INF-based therapy used in treating hepatitis C infected patients in Libya

Daw¹,

Elasifer²,

Dau³

et al. 2013

Virol Discov

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Background: Hepatitis C virus (HCV) therapy has been evolved over years and many parameters were used to evaluate such therapy particularly genotyping. In North Africa and Eastern Mediterranean the genotypes vary from most of world and rarely studies were conducted to assess the influence of such genotypes on the HCV therapy. Aims: This study was designed to determine; the role of HCV genotyping in assessing the efficacy of interferon therapy and to analyze the rates of sustained virological response (SVR) in INF and PegIFN-based regimens according on HCV genotype infecting Libyan patients with chronic hepatitis C infection. Methods: A total of 479 patients with chronic HCV registered at Tripoli Medical Centre were treated with 'INF alfa or PEG-INF-Pegylated INF alfa 2a in combination with ribavirin' for a five year period. These patients were registered and followed up from January 2007 to October 2012. The information were reviewed and data were collected from each patient regarding age, gender, ALT level, and viral load, viral genotype using qualitative PCR. The statistical analysis were carried using SPSS version 11.5. Results: Of the patients studied 86 patients were treated with INF based regimen, only 54% of them had end treatment response (ETR) and 28% had SVR. Off 143 patients treated with peg-INF alfa 2a based therapy; 69% had ETR and 36% had SVR. The SVR of Peg-INF based regimen was higher than INF based regimen in all genotypes except for genotype 4. The relationship between SVR and gender was significant in patients who were given INF based therapy comparable to PEG INF based regimen, though the relationship between SVR and age, basal viral load and basal ALT were also reported in both regimens. Conclusions: HCV genotyping has been found to play an important valuable role in determining the efficacy of Hepatitis C therapy. SVR vary according to the HCV genotype involved. HCV genotype 1 and 4 were found to be the prevalent resistant genotypes infecting Libyan patients. Such findings are particularly important in guiding the clinical therapy of patients infected with hepatitis C virus.

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Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Punjab Province in Pakistan and a phylogenetic analysis

Cited by 33 publications

References 23 publications

Determination of hepatitis C virus genotypes circulating in different districts of Punjab (Pakistan)

Determination of hepatitis C virus genotypes circulating in different districts of Punjab (Pakistan)

Molecular Tracing of Hepatitis C Virus Genotype 1 Isolates in Iran: A NS5B Phylogenetic Analysis with Systematic Review

The role of hepatitis C virus genotyping in evaluating the efficacy of INF-based therapy used in treating hepatitis C infected patients in Libya

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