Molecular epidemiology of feline bordetellosis in two animal shelters in California, USA

Foley, Janet E.; Rand, C.; Bannasch, M. J.; Norris, Carol R.; Milan, Joy

doi:10.1016/s0167-5877(02)00022-3

Cited by 46 publications

(42 citation statements)

References 30 publications

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“…In the cat in which B bronchiseptica was identified, pulsed gel electrophoresis revealed that the organism had a molecular identity that corresponded with that of a vaccinial strain of B bronchiseptica, and the cat was known to have received an intranasal Bordetella vaccine; this pulsed gel electrophoresis technique, including data from this cat, has been previously published. 13 Anaerobic bacterial culture yielded positive results in 3 cats with CRS, and Bacteroides spp were the most common isolates. No fungal organisms were isolated in cats with CRS.…”

Section: Resultsmentioning

confidence: 91%

Assessment of infectious organisms associated with chronic rhinosinusitis in cats

Johnson¹,

Foley²,

Cock³

et al. 2005

javma

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Section: Resultsmentioning

confidence: 91%

Assessment of infectious organisms associated with chronic rhinosinusitis in cats

Johnson¹,

Foley²,

Cock³

et al. 2005

javma

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“…The amounts of LDH were measured spectrophotometrically using a CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega). To calculate the percentage of non-viable cells, 3 ϫ 10 5 HeLa cells seeded on 6-well plates were infected as in the LDH assay described above. After 60 min of infection, cells were washed with cold PBS twice, and then PBS containing 1 mM EDTA was added to the wells.…”

Section: Construction Of Plasmid For Gene Expression In Bordetella Andmentioning

confidence: 99%

“…isolated from a large number of four-legged animals (4) and causes kennel cough in dogs (5) and atrophic rhinitis in swine (6), although most cases of B. bronchiseptica infection are asymptomatic (7). These three Bordetella species colonize the host's respiratory tract.…”

mentioning

confidence: 99%

BopC Is a Novel Type III Effector Secreted by Bordetella bronchiseptica and Has a Critical Role in Type III-dependent Necrotic Cell Death

Kuwae

Matsuzawa

Ishikawa

et al. 2006

Journal of Biological Chemistry

121

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In Bordetella bronchiseptica, the functional type III secretion system (TTSS) is required for the induction of necrotic cell death in infected mammalian cells. To identify the factor(s) involved in necrotic cell death, type III-secreted proteins from B. bronchiseptica were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization tandem mass spectrometry. We identified a 69-kDa secreted protein designated BopC. The gene encoding BopC is located outside of the TTSS locus and is also highly conserved in both Bordetella parapertussis and Bordetella pertussis. The results of a lactate dehydrogenase release assay and terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end-labeling assay demonstrated that BopC is required for necrotic cell death. It has been reported that tyrosine-phosphorylated proteins (PY) of host cells are dephosphorylated during B. bronchiseptica infection in a TTSS-dependent manner. We found that BopC is also involved in PY dephosphorylation in infected host cells. It appears that the necrotic cell death triggered by BopC occurs prior to the PY reduction in host cells, because Bordetella-induced cell death was not affected even in the presence of a dephosphorylation inhibitor. Furthermore, a translocation assay showed that the signal sequence for both secretion into culture supernatant and translocation into the host cell is located in 48 amino acid residues of the BopC N terminus. This report reveals for the first time that a novel type III effector, BopC, is required for the induction of necrotic cell death during Bordetella infection.

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“…Previous studies have shown that the combination of AMX and CLV was effective to treat the feline infection of Mycoplasma spp. (Litster, Wu, & Constable, ), Bordetella bronchiseptica (Foley, Rand, Bannasch, Norris, & Milan, ; Litster et al, ), and Chlamydia psittaci (Sturgess, Gruffydd‐Jones, Harbour, & Jones, ).…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of the amoxicillin–clavulanic acid combination after intravenous and oral administration in cats

Yang

Wang

et al. 2019

Vet Pharm & Therapeutics

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The pharmacokinetic properties of amoxicillin (AMX) and clavulanic acid (CLV) were studied in healthy cats following single intravenous and oral dosage of 10 mg/kg of AMX and 2.5 mg/kg of CLV. The drug concentrations in plasma were determined by a high‐performance liquid chromatographic–tandem mass spectrometry (LC‐MS‐MS) method validated for canine plasma and further subjected to noncompartmental analysis. After intravenous injection, no significant difference (p > 0.05) was found in the volume of distribution of these two compounds. In addition, AMX and CLV were both rapidly eliminated from plasma with a clearance of 0.453 and 0.921 L hr−1 kg−1, respectively; however, a quicker elimination was observed for CLV (p < 0.01). After oral administration, both drugs were characterized by rapid absorption with an absorption half‐life of 1.10 and 0.70 hr for AMX and CLV, respectively. Significant differences were observed between their absorption rates (p < 0.05). However, the oral bioavailabilities of AMX and CLV (75.57% and 98.15%, respectively) were not statistically different (p > 0.05). A total intravenous or oral dose at 12.5 mg/kg of AMX and CLV (4:1) is predicted to be effective for treating those bacterial species isolated from cats with a minimum inhibitory concentration (MIC) of ≤0.25 μg/ml for 12 hr, based on a time above the MIC (T > MIC) of 40%.

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Molecular epidemiology of feline bordetellosis in two animal shelters in California, USA

Cited by 46 publications

References 30 publications

Assessment of infectious organisms associated with chronic rhinosinusitis in cats

Assessment of infectious organisms associated with chronic rhinosinusitis in cats

BopC Is a Novel Type III Effector Secreted by Bordetella bronchiseptica and Has a Critical Role in Type III-dependent Necrotic Cell Death

Pharmacokinetics of the amoxicillin–clavulanic acid combination after intravenous and oral administration in cats

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