Molecular Epidemiology and Antimicrobial Resistance Mechanisms of Methicillin-Resistant<i>Staphylococcus aureus</i>Isolated from Bovine Milk

Türkyılmaz, Süheyla; Tekbiyik, Serten; Oryaşin, Erman; Bozdoğan, Bülent

doi:10.1111/j.1863-2378.2009.01257.x

Cited by 91 publications

(101 citation statements)

References 22 publications

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“…Methicillin resistance is caused by mecA gene, which encodes the penicillin-binding protein 2a with decreased affinity for β-lactam antibiotics. Methicillin-resistant S. aureus should be considered as resistant to all penicillins, cephalosporins, cephems, and other β-lactams such as ampicillin-sulbactam, amoxicillin-clavulanic acid, piperacillin-tazobactam, and carbapenems, regardless of the in vitro test results obtained with these agents (27).…”

Section: Discussionmentioning

confidence: 99%

“…In the study of Bardiau et al (3), among 430 S. aureus isolates collected from bovine mastitis cases, 19 (4.4%) isolates were identified as MRSA, and the presence of the mecA gene was confirmed by PCR for all 19 isolates. The aim of the study of Turkyimaz et al (27) Similar as MRSA, Gram-negative bacilli, able to produce extended-spectrum ß-lactamases (ESBL or AmpC), are an important problem in medicine. The occurrence of these bacteria in food animals and in farm environment was frequently noted (6,11,20,29,30).…”

Section: Discussionmentioning

confidence: 99%

“…Staphylococcus aureus, coagulase negative staphylococci, and Escherichia coli are the main aetiological agents of clinical and subclinical forms of bovine mastitis (2,7,17,23,27,31). Some strains of these bacteria have developed resistance to β-lactam antibiotics, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Weiner¹,

Różańska

Kubajka³

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The aim of study was the preliminary evaluation of the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum ß-lactamases (ESBL) -producing Escherichia coli in 650 milk and inflammatory secretions from cows with clinical or subclinical mastitis. One millilitre of the sample was added to Mueller-Hinton broth supplemented with 6.5% NaCl, Tryptone Soya Broth with cefoxitin and aztreonam, and then to MRSA ID agar. Presumptive MRSA colonies were analysed for the presence of mecA gene. Parallel to MRSA identification, the samples were incubated in buffered peptone water, lauryl tryptose broth and McConkey agar supplemented with cefotaxim for ESBL-producing E. coli isolation. These bacteria were identified using API Rapid 32 E and the ability of ESBL production was initially established using disc test D68C and confirmed by MIC technique using Sensititre ESBL plates. The primers (blaCTX, blaTEM, blaSHV, and blaCMY-2-group) for the detection of some of the genes encoding ESBL production were used. The 45 strains of S. aureus with mecA gene and 41 strains of E. coli with blaTEM gene were detected.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Weiner¹,

Różańska

Kubajka³

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Por ello, las cepas de SARM deberían ser consideradas resistentes a todas las penicilinas, cefalosporinas, cefemes y otros β-lactámicos, como las combinaciones de ampicilina/sulbactam, amoxicilina/ácido clavulánico, ticarcilina/ácido clavulánico, piperacilina/tazobactam y tecarbapenemes, independiente de los resultados obtenidos in vitro con estos fármacos. Además, estos microorganismos pueden ser resistentes a los antimicrobianos más comúnmente usados, incluyendo aminoglucósidos, macrólidos, cloranfenicol, tetraciclinas y fluoroquinolonas 24 .…”

Section: Microbiologíaunclassified

Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Galarce

Muñoz

Jara

et al. 2016

Rev. chil. infectol.

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“…Kırkan et al [12] reported that 60% of the oxacillin resistance was detected in S. aureus strains studied in Aydın region. Türkyılmaz et al [13] reported that cefoxitin resistance was 17.2% in S. aureus strains in the same region. In this study, methicillin resistance was determined in the ratio of 2.9% for coagulase positive strains and 16.3% for coagulase negative strains.…”

Section: It Has Been Shown That Cns (S Haemolyticus S Hyicus S Smentioning

confidence: 99%

Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi

Savaşan¹,

Kırkan²,

Erbaş³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleSavaşan S, Kırkan Ş, Erbaş G, Parın U, Çiftci A: Determination of virulence factors of Staphylococci isolated from bovine mastitis. Kafkas Univ Vet Fak Derg, 23 (6): 947-952, 2017. DOI: 10.9775/kvfd.2017 AbstractStaphylococcal mastitis is one of the major economic problems of cattle. The aim of this study was to identify Staphylococcus species that cause mastitis, to examine the virulence factors of these species and to determine the relation between these factors with the pathogenic and nonpathogenic species. In the study, 37 S. aureus, 13 S. hyicus, 9 S. simulans, 8 S. chromogenes, 5 S. lentus, 5 S. epidermidis, 2 S. haemolyticus, 2 S. hominis, 1 S. auricularis, 1 S. warneri, 1 S. sciuri were isolated and identified. The 41.6% of strains were determined as coagulase positive. In the coagulase positive strains, the rate of protein A, DNase, TNase, capsul, hemolyse, staphylokinase, slime (in agar), biofilm (microdilution) and hemagglutination characteristics were found 71.4%, 48.6%, 11.4%, 40%, 97.1%, 40%, 28.6%, 37.1% and 17.1%, respectively. In the coagulase negative strains, the rate of these characteristics were found 10.2%, 12.2%, 2%, 8.2%, 82%, 32.7%, 12.2%, 12.2% and 10.2%, respectively. The methicillin resistance rates in the coagulase positive and negative strains were determined as 2.9 and 16.3%. In conclusion, it was of the opinion that animals are potential carriers of staphylococcus strains that are pathogen for human. Keywords: Bovine, Mastitis, Staphylococci, Virulence Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi ÖzetStafilokokal mastitisler sığırlar için en önemli ekonomik problemlerden birisidir. Bu çalışmada mastitise neden olan stafilokok türlerinin sahip olduğu virülens faktörlerinin belirlenmesi ve patojenik ile apatojenik türler arasında bu faktörlerin ilişkisinin araştırılması amaçlanmıştır. Çalışma kapsamında 37 S. aureus, 13 S. hyicus, 9 S. simulans, 8 S. chromogenes, 5 S. lentus, 5 S. epidermidis, 2 S. haemolyticus, 2 S. hominis, 1 S. auricularis, 1 S. warneri ve 1 S. sciuri izole ve identifiye edilmiştir. Bu izolatların %41.6'sı koagülaz pozitif olarak belirlenmiştir. Koagülaz pozitif izolatların protein A, DNase, TNase, kapsül, hemoliz, stafilokinaz, slaym (agar'da), biyofilm (mikrodilüsyon) ve hemaglütinasyon özellikleri sırasıyla %71.4, %48.6, %11.4, %40, %97.1, %40, %28.6, % 37.1 ve %, 17.1 olarak tespit edilmiştir. Koagülaz negatif izolatlarda ise bu oranlar sırasıyla %10.2, %12.2, %2, %8.2, %82, %32.7, %12.2, %12.2 ve %10.2 bulunmuştur. Koagülaz pozitif ve negatif izolatlar arasında metisilin direnç oranı %2.9 ve %16.3 olarak saptanmıştır. Sonuç olarak, stafilokok taşıyıcısı hayvanların insanlar için muhtemel infeksiyon kaynağı olabileceği sonucuna varılmıştır.

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Molecular Epidemiology and Antimicrobial Resistance Mechanisms of Methicillin-ResistantStaphylococcus aureusIsolated from Bovine Milk

Cited by 91 publications

References 22 publications

Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Occurrence and characterisation of MRSA and extended-spectrum ß-lactamases producing Escherichia coli isolated from mastitic cows’ milk

Detección del gen mecA en cepas de Staphylococcus coagulasa positiva aisladas desde gatos

Sığır İzolatı Stafilokokların Virülens Faktörlerinin Belirlenmesi

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