Molecular epidemiology and a loop-mediated isothermal amplification method for diagnosis of infection with rabies virus in Zambia

Muleya, Walter; Namangala, Boniface; Mweene, Aaron S.; Zulu, L. N. K.; Fandamu, Paul; Banda, Douglas M.; Kimura, Takashi; Sawa, Hirofumi; Ishii, Akihiro

doi:10.1016/j.virusres.2011.09.010

Cited by 30 publications

(27 citation statements)

References 38 publications

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“…It is clear that the LAMP assay system represents a promising technology in both the diagnosis and post-elimination surveillance of NTDs. In addition, because many of these diseases are zoonotic vector-borne, LAMP has also been applied to the detection of pathogens in vectors [40] and reservoir animals [41,42], hinting at the potential for LAMP to be useful in both epidemiological surveillance and control of infection in endemic countries.…”

Section: Developmental Prospects Of New Lamp Reagentsmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Mori¹,

Kanda²,

Notomi³

2013

Journal of Infection and Chemotherapy

234

175

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Loop-mediated isothermal amplification (LAMP) is an established technology that continues to attract the attention of researchers in many fields. Research and development efforts on LAMP technology in recent years have focused on two major areas; first, the study of its clinical application as an approved in vitro diagnostics tool in Japan and certain other countries; and second, research aimed at further simplifying the LAMP test process. This review provides an overview of the status of LAMP on these two topics by summarizing research work conducted, in the main, after our previous review article.

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Section: Developmental Prospects Of New Lamp Reagentsmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Mori¹,

Kanda²,

Notomi³

2013

Journal of Infection and Chemotherapy

234

175

View full text Add to dashboard Cite

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“…2 Recent studies have highlighted the epidemiology of rabies in southern Africa and the action needed for elimination to take place within the region. [3][4][5][6][7] Rabies has been reported since 1887 in Namibia, where it mainly occurs in the north where 70% of the human population resides, and involves domestic and wild animals. Rabies primarily affects jackal, kudu and cattle in the commercial farming central regions.…”

Section: Introductionmentioning

confidence: 99%

Knowledge and practices of residents in two north Namibian towns with regard to rabies and pet care

Haimbodi

Mavenyengwa

Noden

2014

Southern African Journal of Infectious Diseases

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“…The automated NASBA enables easy and rapid testing of samples and has been reported to have a higher sensitivity than conventional PCR assays for detection of rabies viral RNA in antemortem saliva and CSF samples [67]. Loop-Mediated Isothermal Amplification (LAMP) is an alternative method of amplification of DNA with high specificity and efficiency and without the need for thermal cycling, which has been used for rabies diagnosis [68, 69]. Since this method does not have the technological requirements of thermal cycling used in RT-PCR, it can be used to develop surveillance protocols where testing can take place in the field or in less sophisticated laboratories [32].…”

Section: Newer Diagnostic Tests For Rabiesmentioning

confidence: 99%

Laboratory Diagnosis of Human Rabies: Recent Advances

Mani

Madhusudana

2013

The Scientific World Journal

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Rabies, an acute progressive, fatal encephalomyelitis, transmitted most commonly through the bite of a rabid animal, is responsible for an estimated 61,000 human deaths worldwide. The true disease burden and public health impact due to rabies remain underestimated due to lack of sensitive laboratory diagnostic methods. Rapid diagnosis of rabies can help initiate prompt infection control and public health measures, obviate the need for unnecessary treatment/medical tests, and assist in timely administration of pre- or postexposure prophylactic vaccination to family members and medical staff. Antemortem diagnosis of human rabies provides an impetus for clinicians to attempt experimental therapeutic approaches in some patients, especially after the reported survival of a few cases of human rabies. Traditional methods for antemortem and postmortem rabies diagnosis have several limitations. Recent advances in technology have led to the improvement or development of several diagnostic assays which include methods for rabies viral antigen and antibody detection and assays for viral nucleic acid detection and identification of specific biomarkers. These assays which complement traditional methods have the potential to revolutionize rabies diagnosis in future.

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Molecular epidemiology and a loop-mediated isothermal amplification method for diagnosis of infection with rabies virus in Zambia

Cited by 30 publications

References 38 publications

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Knowledge and practices of residents in two north Namibian towns with regard to rabies and pet care

Laboratory Diagnosis of Human Rabies: Recent Advances

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