Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Mori, Yasuyoshi; Kanda, Hidetoshi; Notomi, Tsugunori

doi:10.1007/s10156-013-0590-0

Cited by 234 publications

(192 citation statements)

References 61 publications

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“…In the laboratory, amplified products can be observed directly by naked eye in the reaction tube by adding SYBR Green I stain or picogreen dye, or by gel electrophoresis under UV trans-illuminator to see a ladder-like structure, or by turbidity assay based on chemical reactions [15]- [17]. Currently, it seems that only fluorescent dye is feasible for on-site application.…”

Section: Lamp Results Judgmentsmentioning

confidence: 99%

“…Due to the nature of fast, accurate, and cost-effective, LAMP has been widely and mainly applied in the rapid molecular diagnosis of pathogens, bacteria and viruses, GMO ingredients, tumor detection, and embryo sex identification [15]- [17]. The number of studies about LAMP has been increasing every year.…”

Section: Lamp: a Novel Gmo Detection Methodsmentioning

confidence: 99%

“…The number of studies about LAMP has been increasing every year. Its principle and application have been well described by many reviews [15]- [17], which therefore will not be included in this paper.…”

Section: Lamp: a Novel Gmo Detection Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

Li¹,

Ji²,

Zhao³

et al. 2015

OALib

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As more and more genetically modified (GM) crops are approved for commercialization and planting, safety issues of GM crops have become hot topics worldwide. For both regulatory and academic purposes, development of rapid, economic and effective on-site detection methods for GM components is indispensible. Up to now, the most effective and sensitive techniques used for GMO detection are based on polymerase chain reaction (PCR). PCR method needs expensive, heavy instruments and gel electrophoresis. Therefore, it is commonly used in laboratory test, and unsuitable for on-site detection. Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification technique, has been extensively used in many areas such as food safety and clinic diagnosis. Advantageous characteristics of LAMP, such as high specificity and sensitivity, simple operation, low cost, eye visualization, particularly free of special equipment, render it with high potential to be used for GMO on-site detection. In this review, we summarized current status of the application of LAMP in GMO detection, and discussed possible improvements needed for its adaptability regarding to on-site GMO detection. Hopefully, the information present here would facilitate the practical risk assessment of GMO.

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Section: Lamp Results Judgmentsmentioning

confidence: 99%

Section: Lamp: a Novel Gmo Detection Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

Li¹,

Ji²,

Zhao³

et al. 2015

OALib

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show abstract

“…Similar accounts of cross-reactivity have not yet been reported, so further data will be useful to monitor the specificity of PCR in different populations. 40 . LAMP has been successfully applied in resource limited-settings for the detection of pathogens 40 .…”

Section: Pcrmentioning

confidence: 99%

“…40 . LAMP has been successfully applied in resource limited-settings for the detection of pathogens 40 .…”

Section: Pcrmentioning

confidence: 99%

The laboratory diagnosis of Strongyloides stercoralis

Watts¹,

Robertson²,

Bradbury

2016

Microbiol. Aust.

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It is estimated that over 30million people worldwide are infected by the nematode, Strongyloides stercoralis1. It is endemic in sub-tropical and tropical parts of Australia, with high rates of infection documented in some indigenous communities2. Due to the potential for chronic autoinfection, that may persist for decades, migration leads to the presence of the infection in non-endemic areas1. Transmission to humans is generally through the penetration of larvae through the skin, following contact with faecally contaminated soil1. Disease severity ranges from asymptomatic chronic carriage to an overwhelming illness, where large numbers spread throughout the body, usually triggered by immunosuppression1.

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Die exponentielle isotherme Amplifikation von Nukleinsäuren und Assays zur Detektion von Proteinen, Zellen, kleinen Molekülen und Enzymaktivitäten: Anwendungen für EXPAR

Reid¹,

Zhang

2018

Angewandte Chemie

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Isotherme exponentielle Amplifikationstechniken, wie die Strangverdrängungsamplifikation (SDA), die Rolling‐Circle‐Amplifikation (RCA), die Loop‐vermittelte isotherme Amplifikation (LAMP), die Nukleinsäuresequenz‐basierte Amplifikation (NASBA), die Helikase‐abhängige Amplifikation (HDA) und die Rekombinase‐Polymerase‐Amplifikation (RPA) besitzen großes Anwendungspotential für Vor‐Ort‐Analysen, patientennahe Labordiagnostik und In‐situ‐Assays. Diese Amplifikationsmethoden benötigen im Gegensatz zur Polymerasekettenreaktion (PCR) keine Temperaturzyklen, liefern aber vergleichbare Ausbeuten. In diesem Kurzaufsatz stellen wir die neuesten Entwicklungen der exponentiellen Amplifikationsreaktion (EXPAR) zur Detektion von Nukleinsäuren, Proteinen, Enzymaktivitäten, Zellen und Metallionen vor. Mithilfe der Verwendung von Fluoreszenz, Kolorimetrie, Chemilumineszenz, Raman und elektrochemischer Verfahren konnte bereits eine hoch sensitive Detektion einer Vielzahl verschiedener Ziele (Targets) realisiert werden. Dennoch bleiben offene Fragen, wie insbesondere die der unerwünschten Hintergrundamplifikation aus unspezifischen Templat‐Interaktionen, die es zu lösen gilt, um die Entwicklung der isothermen und exponentiellen Amplifikationstechniken weiter voranzutreiben.

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Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Cited by 234 publications

References 61 publications

Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

Loop-Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

The laboratory diagnosis of Strongyloides stercoralis

Die exponentielle isotherme Amplifikation von Nukleinsäuren und Assays zur Detektion von Proteinen, Zellen, kleinen Molekülen und Enzymaktivitäten: Anwendungen für EXPAR

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