Molecular epidemiological update of Peste des Petits Ruminants virus (PPRV) in Ethiopia

Rume, Veronica Nwankpa; Dundon, William G.; Belay, Gurja; Baziki, Jean de Dieu; Diakité, A.; Paul, Ankeli; Tessema, Yebchaye Degefa; Nwankpa, Nick; Gizaw, Daniel; Cattoli, Giovanni; Bodjo, Sanne Charles; Tessema, Tesfaye Sisay

doi:10.1016/j.vetmic.2019.07.006

Cited by 7 publications

(9 citation statements)

References 18 publications

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“…In Ethiopia, lineage IV is slowly replacing PPRV lineage III [ 10 , 11 ]. Because of its economic burden in many developing countries, PPR is a priority animal disease that has been targeted for global eradication by 2030 by the Food and Agriculture Organization of the United Nations (FAO) and the World Organization for Animal Health (OIE) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020

Mohammed

Chibssa²,

Terfa

et al. 2022

Advances in Virology

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Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs (n = 24) were collected from sheep (n = 7) and goats (n = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR. Positive samples were confirmed by conventional RT-PCR followed by sequencing of a partial nucleoprotein (N) gene segment. Results revealed that 54% (n = 13/24) of the tested samples were PPRV-positive Phylogenetic analysis revealed that the viruses belonged to lineage IV and lineage II. The lineage IV viruses were similar, although not identical, to other lineage IV viruses previously reported in Ethiopia and other East African countries while the lineage II viruses have been reported for the first time in Ethiopia showed a high nucleotide identity (99.06%) with the vaccine (Nigeria 75/1) that is currently used in Ethiopia for the prevention of PPR. Further investigations are therefore recommended in order to fully understand the true nature of the lineage II PPRVs in Ethiopia.

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Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020

Mohammed

Chibssa²,

Terfa

et al. 2022

Advances in Virology

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“…2 and 3 ). Of the 64 PPRV-positive samples collected from 2000 to 2009 that were sequenced by Kwiatek et al [ 13 ], only two belonged to lineage III, leading the authors to conclude that this lineage was being progressively replaced by lineage IV viruses, a situation that has also been reported recently in Ethiopia [ 49 ].…”

Section: Sudanmentioning

confidence: 92%

“…2 and 3). However, two more recent studies on samples from Ethiopia from 2011, 2014 and 2017 only detected lineage IV PPRVs, suggesting that the lineage III viruses may have been replaced by lineage IV viruses [48,49]. Currently, there are five full genome sequences of PPRVs from Ethiopia available in GenBank (nos.…”

Section: Ethiopiamentioning

confidence: 99%

“…It now appears that lineage I viruses are no longer circulating, as this lineage has not been identified since 2001 [69]. Some argue that this lineage has been replaced by lineage II and IV viruses across the region [49,81]. Lineage II is predominantly present in West Africa, although it has recently been reported in the DRC and Tanzania.…”

Section: Lineages In Africamentioning

confidence: 99%

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Peste des petits ruminants in Africa: a review of currently available molecular epidemiological data, 2020

2020

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Small ruminants (e.g., sheep and goats) contribute considerably to the cash income and nutrition of small farmers in most countries in Africa and Asia. Their husbandry is threatened by the highly infectious transboundary viral disease peste des petits ruminants (PPR) caused by peste-des-petits-ruminants virus (PPRV). Given its social and economic impact, PPR is presently being targeted by international organizations for global eradication by 2030. Since its first description in Côte d’Ivoire in 1942, and particularly over the last 10 years, a large amount of molecular epidemiological data on the virus have been generated in Africa. This review aims to consolidate these data in order to have a clearer picture of the current PPR situation in Africa, which will, in turn, assist authorities in global eradication attempts.

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“…Lineage IV is found in Southeast Asia, Middle East and North Africa [23,44,[58][59][60]. Incursions of PPRV lineages have been also reported, for instance lineage II and IV were found in East Africa and lineage IV in Ethiopia [12,[61][62][63][64]. Recent outbreaks of PPR in Bulgaria, Georgia and the Marmara region in Turkey increase the threat to Europe [65][66][67].…”

Section: Epidemiology Of Peste Des Petits Ruminantsmentioning

confidence: 99%

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

et al. 2020

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Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores. Diagnosis remains a cornerstone to the Peste des petits ruminants Global Control and Eradication Strategy, an initiative of the World Organisation for Animal Health and the Food and Agriculture Organisation. The present review presents the peste des petits ruminants diagnostic landscape, including the practicality of commercially available diagnostic tools, prototype tests and opportunities for new technologies. The most common peste des petits ruminants diagnostic tools include; agar gel immunodiffusion, counter-immunoelectrophoresis, enzyme-linked immunosorbent assays, reverse transcription polymerase chain reaction either gel-based or real-time, reverse transcription loop-mediated isothermal amplification, reverse transcription recombinase polymerase amplification assays, immunochromatographic lateral flow devices, luciferase immunoprecipitation system and pseudotype-based assays. These tests vary in their technical demands, but all require a laboratory with exception of immunochromatographic lateral flow and possibly reverse transcription loop-mediated isothermal amplification and reverse transcription recombinase polymerase amplification assays. Thus, we are proposing an efficient integration of diagnostic tests for rapid and correct identification of peste des petits ruminants in endemic zones and to rapidly confirm outbreaks. Deployment of pen-side tests will improve diagnostic capacity in extremely remote settings and susceptible wildlife ecosystems, where transportation of clinical samples in the optimum cold chain is unreliable.

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Molecular epidemiological update of Peste des Petits Ruminants virus (PPRV) in Ethiopia

Cited by 7 publications

References 18 publications

Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020

Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020

Peste des petits ruminants in Africa: a review of currently available molecular epidemiological data, 2020

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

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