Molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from China evaluated by nested PCRs

Yu, Peifa; Niu, Qingli; Liu, Zhijie; Yang, Jifei; Chen, Ze; Guan, Guiquan; Liu, Guangyuan; Luo, Jianxun; Yin, Hong

doi:10.1016/j.actatropica.2016.02.027

Cited by 25 publications

(16 citation statements)

References 49 publications

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“…A high prevalence (36%) of active infection was recently detected in dogs infested by R. sanguineus in north-eastern Arizona [ 25 ]. In China, serological and PCR-based study results for Ehrlichia and Anaplasma infection have been reported concerning ticks, animals and humans [ 26 , 27 , 28 , 29 ]. This study reports the first detection of H. longicornis and R .…”

Section: Discussionmentioning

confidence: 99%

Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China

et al. 2017

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To understand the epidemiology of tick infestation and tick-borne diseases in pet dogs in south-eastern China and to develop a reference for their prevention and treatment, we collected 1550 ticks parasitizing 562 dogs in 122 veterinary clinics from 20 cities of south-eastern China. Dogs were tested for common tick-borne pathogens; collected ticks were identified and processed for the detection of tick-borne pathogens. The use of an in vitro ELISA diagnostic kit for antibody detection (SNAP®4Dx® Plus) on dog sera found the infection rates with Borrelia burgdorferi sensu lato, Ehrlichia canis, and Anaplasma spp. to be 0.4%, 1.3% and 2.7%, respectively. By using a specific ELISA method, the infection rate with Babesia gibsoni was 3.9%. Rhipicephalus sanguineus sensu lato, Haemaphysalis longicornis and Rhipicephalus haemaphysaloides were the major tick species identified on pet dogs. PCR tests were conducted to detect five tick-borne pathogens in 617 ticks. The infection rate was 10.2% for E. canis, 3.4% for Anaplasma platys, 2.3% for B. gibsoni, 0.3% for B. burgdorferi s.l. and 0% for Babesia canis. Some ticks were co-infected with two (1.46%) or three pathogens (0.16%). These results indicate the infestation of pet dogs by ticks infected with tick-borne pathogens in south-eastern China, and the need for effective treatment and routine prevention of tick infestations in dogs.

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Section: Discussionmentioning

confidence: 99%

Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China

et al. 2017

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“…are obligate intracellular bacteria residing within the cytoplasmic vacuoles of monocytes, granulocytes, or platelets of humans and animals, and can cause illnesses with fever, leukopenia, and thrombocytopenia (Dumler and Bakken, 1995). Serological and molecular evidences show a wide distribution of the bacteria infection in ticks, animals, and humans (Yu et al, 2016). In China, E. chaffeensis was detected in Amblyomma testudinarium , H. yeni , and I. persulcatus (Cao et al, 2000; Zhang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…E. canis has been identified in Rhipicephalus sanguineus and Rhipicephalus microplus (Wen et al, 2003), and human infection usually presents fever, malaise, thrombocytopenia, and lymphopenia (Johnson et al, 2015). E. muris has been detected in R. microplus in Hunan (Yu et al, 2016). In the present study, we first detected E. muris in I. persulcatus collected from Jilin and Heilongjiang provinces of northeastern China, with a prevalence of 1.9–4.3%, showing that I. persulcatus may be a vector for this bacterium in northeastern China.…”

Section: Discussionmentioning

confidence: 99%

“…Other species that infect animals include A. marginale , Anaplasma centrale , A. ovis , E. canis , and Ehrlichia minasensis (Wen et al, 2003; Kocan et al, 2015; Cabezas-Cruz et al, 2016). Several species have been described in China, such as A. ovis , Anaplasma bovis , and A. platys in Gansu province (Li et al, 2016), A. centrale and E. canis in Fujian province (Ge et al, 2016; Yu et al, 2016), and E. chaffeensis in Guangxi province (Yang et al, 2015b). In Asia, Ixodes persulcatus is considered the primary vector of A. phagocytophilum and E. muris (Jin et al, 2012; Ivanova et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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“…This is especially dictated by the emergent number of patients with relapsing LD symptoms appearing after completion of conventional treatments. The exact mechanism of this phenomenon called PTLDS (post-treatment LD syndrome) still is not clear and awaits further exploration (Klempner et al 2013, Center for Disease Control andPrevention 2014;Johnson et al 2014;Marques et al 2014;Stricker and Johnson 2014;Yu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Reciprocal cooperation of phytochemicals and micronutrients against typical and atypical forms ofBorreliasp.

2017

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Molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from China evaluated by nested PCRs

Cited by 25 publications

References 49 publications

Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China

Epidemiological survey of ticks and tick-borne pathogens in pet dogs in south-eastern China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Reciprocal cooperation of phytochemicals and micronutrients against typical and atypical forms ofBorreliasp.

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