Molecular epidemiological characterization of<i>Brucella</i>isolates from sheep and yaks in northwest China

Cao, Xiaoan; Li, Z.; Liu, Z.; Fu, Bao‐Quan; Liu, Y.; Shang, Youjun; Zhou, Jinlin; Jing, Zhaoyang

doi:10.1111/tbed.12777

Cited by 20 publications

(19 citation statements)

References 25 publications

(58 reference statements)

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“…In the last 12 years, brucellosis has re-emerged in most regions of China, showing an annual increase in animal and human infections during this period [21,22]. The prevalence and dissemination of animals (sheep) brucellosis were very serious, resulting to extensive human brucellosis and incidence rate of human brucellosis in Inner Mongolia Autonomous Region was the ranked top three in Chinese provinces [19].…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Liu

Wang²,

et al. 2020

Emerging Microbes & Infections

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In the present study, a total of 1102304 serum samples were collected to detected human brucellosis between the years 2012 and 2016 in Inner Mongolia. Overall, an average of 3.79% anti-Brucella positive in Inner Mongolia was presented but the range of positive rates were among 0.90 to 7.07% in 12 regions. Seroprevalence of human brucellosis increased gradually from 2012 to 2016. However, the incidence rate of human brucellosis showed a declining trend. One hundred and seven Brucella strains were isolated and identified as B. melitensis species, and B. melitensis biovar 3 was the predominant biovar. MLVA-11 genotypes 116 was predominant and had crucial epidemiology to the human population. All 107 strains tested were sorted into 75 MLVA-16 genotypes, with 54 single genotypes representing unique isolates. This result revealed that these Brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 21 shared genotypes among two to four strains, confirming the occurrence of crossinfection and multiple outbreaks. Extensive genotype-events were observed between strains from this study and Kazakhstan, Mongolia, and Turkey, these countries were key members of the grassland silk road. Long-time trade in small ruminants (sheep) in these countries has possibly promoted the spread of Brucella spp. in these regions.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Liu

Wang²,

et al. 2020

Emerging Microbes & Infections

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“…In the previous study [ 10 ], sixty-six isolates were collected from northwest China; 13, 26, 17, and 10 were from Gansu, Inner Mongolia, Xinjiang, and Qinghai provinces, respectively ( Table 1 ). Brucella were reproduced in BBL™ Brucella Broth (BD, USA) with 5% horse serum at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Genetic Characterization of Animal Brucella Isolates from Northwest Region in China

Cao

Shang

Liu

et al. 2018

BioMed Research International

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Animal brucellosis is a reemerging disease in China, particular in northwest China. The Brucella species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on Brucella. In this study, MLST was used to analyze the characterization of Brucella from sheep and yaks during 2015 and 2016. All 66 isolates were collected from northwest China, including Inner Mongolia, Xinjiang, Qinghai, and Gansu provinces. Isolates were cultured on Brucella agar medium and identified by MLST. MLST identified five ST types: ST8 (n = 55), ST7 (n = 2), ST3 (n = 5), ST1 (n = 2), and ST14 (n = 2). This analysis revealed that B. melitensis isolates exhibited high single genotypes (ST8) in the most northwest China. MLST of isolates provides helpful information on understanding genetic characterization of Brucella in northwest China.

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“…There is high prevalence of animal brucellosis in the western provinces of China [12]. For this reason, simple and rapid techniques are needed for the detection of Brucella in veterinary clinics.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection

Gumaa

Cao

et al. 2019

Molecular and Cellular Probes

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A B S T R A C TBrucellosis is a worldwide re-emerging zoonosis. It has an economic impact due to abortion and loss of fertility in livestock. In this study, Real-time recombinase polymerase amplification (RT-RPA-BP26) targeting Brucella spp. bp26 gene and Lateral flow dipstick (LFD-RPA-IS711) combined with SYBR-Green recombinase polymerase amplification (RPA) targeting insertion sequence IS711 region of Brucella spp. bp26 gene, was developed to detect Brucella spp. from different sample types in domestic animals. The sensitivity and specificity of the two developed RPAs were compared with real-time PCR, PCR, and Rose Bengal Plate Test (RBPT). The analytical sensitivity and detection limit of Real-time RPA and LFD RPA were four and six copies per reaction respectively. The detection of six colony forming units (CFU) of the bacteria-bearing construct with the target sequence was within 20 min at 40°C for Real-time RPA and 37°C for LFD RPA. The LFD RPA could work at temperatures between 30 and 35°C and could be completed within 10-30 min. No significant differences were observed when comparing the results from Real-time RPA and LFD RPA to Real-time PCR and PCR. Both methods showed no cross reactivity with Chlamydia abortus, Toxoplasma gondii, Salmonella typhimurium, and Escherichia coli. In conclusion, RPA is a useful and convenient field and point of care test for brucellosis.

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Molecular epidemiological characterization ofBrucellaisolates from sheep and yaks in northwest China

Cited by 20 publications

References 25 publications

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Seroprevalence of human brucellosis and molecular characteristics of Brucella strains in Inner Mongolia Autonomous region of China, from 2012 to 2016

Genetic Characterization of Animal Brucella Isolates from Northwest Region in China

Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection

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